Expression data of MYB36 mutants
Ontology highlight
ABSTRACT:
ORGANISM(S): Arabidopsis thaliana
PROVIDER: GSE62993 | GEO | 2015/06/30
SECONDARY ACCESSION(S): PRJNA266379
REPOSITORIES: GEO
Similar Datasets
Project description:We have identified the causal genes, which is MYB36, of ionome mutants. To identify the genes regulated by MYB36, RNAs prepared from two alleles were applied for the micoarray analysis. Two-weeks old roots grown under normal conditions were applied for the microarray analysis
2015-06-30 | E-GEOD-62993 | biostudies-arrayexpress
Project description:MYB36 has been identified as a new transcription factor (TF) that may be involved in isoflavonoid metabolism and stress responses in the model legume Lotus japonicus. A series of transcriptomic and metabolomic approaches have been carried out to attempt to identify the specific genes that can be under the control of MYB36 TF in this plant. For this purpose, transcriptomic data were obtained for wild type (WT) and two different lines of homozygous myb36 mutant plants, under two different stress conditions shown to be related with isoflavonoid metabolism: UV-B irradiation and salt stress. Results obtained showed a completely different and almost reciprocal picture of the global transcriptomic of the WT plants under both types of stress situations. Further comparison of up- and down- regulated genes revealed a very similar response of WT and mutant plants under UV-irradiation. In contrast, a completely different response was detected in salt stress between WT and mutant plants. None of the genes modulated by salt stress in myb36 mutants were also modulated by UV-B irradiation. Furthermore, most of the genes modulated by salt stress in the mutants corresponded to genes specifically involved in isoflavonoid but not flavonoid biosynthesis, thus illustrating on the genes that can be under the specific control of MYB36. These results identify for the first time MYB36 as specifical TF in the regulation of (iso)flavonoid biosynthesis, a class of compound specifically produced by legume plants. In addition, the results obtained revealed MYB36 as a crucial TF in the response to salt stress in legume plants.
2026-04-24 | GSE283420 | GEO
Project description:MYB36 has been identified as a new transcription factor (TF) that may be involved in isoflavonoid metabolism and stress responses in the model legume Lotus japonicus. A series of transcriptomic and metabolomic approaches have been carried out to attempt to identify the specific genes that can be under the control of MYB36 TF in this plant. For this purpose, transcriptomic data were obtained for wild type (WT) and two different lines of homozygous myb36 mutant plants, under two different stress conditions shown to be related with isoflavonoid metabolism: UV-B irradiation and salt stress. Results obtained showed a completely different and almost reciprocal picture of the global transcriptomic of the WT plants under both types of stress situations. Further comparison of up- and down- regulated genes revealed a very similar response of WT and mutant plants under UV-irradiation. In contrast, a completely different response was detected in salt stress between WT and mutant plants. None of the genes modulated by salt stress in myb36 mutants were also modulated by UV-B irradiation. Furthermore, most of the genes modulated by salt stress in the mutants corresponded to genes specifically involved in isoflavonoid but not flavonoid biosynthesis, thus illustrating on the genes that can be under the specific control of MYB36. These results identify for the first time MYB36 as specifical TF in the regulation of (iso)flavonoid biosynthesis, a class of compound specifically produced by legume plants. In addition, the results obtained revealed MYB36 as a crucial TF in the response to salt stress in legume plants.
2026-04-24 | GSE283584 | GEO
Project description:Stem cells are defined by their ability to self-renew and produce daughter cells that proliferate and mature. These maturing cells transition from a proliferative state to a terminal state through the process of differentiation. In the Arabidopsis thaliana root the transcription factors SCARECROW and SHORTROOT regulate specification of the bi-potent stem cell that gives rise to the cortical and endodermal progenitors. Subsequent progenitor proliferation and differentiation generates mature endodermis, marked by the Casparian Strip: a cell wall modification that prevents ion diffusion into and out of the vasculature. We identified a transcription factor, MYB36 that regulates the transition from proliferation to differentiation in the endodermis. We show that SCARECROW directly activates MYB36 expression, which in turn directly regulates essential Casparian Strip formation genes. In addition, MYB36 represses extra divisions within the endodermis. Our results demonstrate that MYB36 is a critical positive regulator of differentiation and negative regulator of cell proliferation. 12 samples analyzed: 3 biological replicates each from 1) wild type (Col-0) whole root, 2) mutant (myb36-1) whole root, 3) wild type (Col-0) sorted endodermis, 4) mutant (myb36-1) sorted endodermis
2015-07-20 | E-GEOD-70584 | biostudies-arrayexpress
Project description:In our previous work, we found that the root exudates of sgn3 myb36 promoted the colonization of CHA0 on roots. Through LC-MS, we identified a large amount of glutamine (Gln) in the root exudates of sgn3 myb36. Therefore, we aim to use RNA-seq to uncover whether the root exudates of sgn3 myb36 and Gln have the same regulatory effects on CHA0. By conducting differential analysis with the CK (CHA0 treated with wild-type root exudates), we hope to identify the specific regulatory mechanisms of sgn3 myb36 and Gln on CHA0.
2024-11-06 | GSE280681 | GEO
Project description:Stem cells are defined by their ability to self-renew and produce daughter cells that proliferate and mature. These maturing cells transition from a proliferative state to a terminal state through the process of differentiation. In the Arabidopsis thaliana root the transcription factors SCARECROW and SHORTROOT regulate specification of the bi-potent stem cell that gives rise to the cortical and endodermal progenitors. Subsequent progenitor proliferation and differentiation generates mature endodermis, marked by the Casparian Strip: a cell wall modification that prevents ion diffusion into and out of the vasculature. We identified a transcription factor, MYB36 that regulates the transition from proliferation to differentiation in the endodermis. We show that SCARECROW directly activates MYB36 expression, which in turn directly regulates essential Casparian Strip formation genes. In addition, MYB36 represses extra divisions within the endodermis. Our results demonstrate that MYB36 is a critical positive regulator of differentiation and negative regulator of cell proliferation.
2015-07-20 | GSE70584 | GEO
Project description:adt07-03_mir398-mutants - mir398 mutant analysis - Defining new targets of miR398 by transcriptome analysis of two miR398 mutant alleles. - Plants were grown on soil in culture chambers under the following conditions, a day/night cycle of 16/8 hours was applied, light intensity was around 100-150 umol m-2 s-1, temperature day/night was 20/15°C. Plants were harvested 17 days later and RNAs were then extracted. The experiment was repeated twice. Keywords: gene knock out
2008-07-11 | GSE10672 | GEO
Project description:3 mutants from the clpex mouse mutant line were exome sequenced to identify candidate causal variants.
2019-05-30 | GSE131920 | GEO
Project description:adt07-03_mir398-mutants - mir398 mutant analysis - Defining new targets of miR398 by transcriptome analysis of two miR398 mutant alleles. - Plants were grown on soil in culture chambers under the following conditions, a day/night cycle of 16/8 hours was applied, light intensity was around 100-150 umol m-2 s-1, temperature day/night was 20/15°C. Plants were harvested 17 days later and RNAs were then extracted. The experiment was repeated twice. Keywords: gene knock out 4 dye-swap - CATMA arrays
2010-05-25 | E-GEOD-10672 | biostudies-arrayexpress