Dataset Information


Expression data from glioblastoma stem-like cells (GSCs) and astrocyte co-cultured GSCs

ABSTRACT: consequences of astrocytes on GSCs, gene expression profiles generated from glioblastoma stem-like cells grown alone (mono-culture) and compared to those generated 48h after the initiation of co-culture with astrocytes We used microarrays show that astrocytes are capable to modify via a paracrine mechanism GSC gene expression and thus phenotype. Overall design: GSCs were seeded into 6-wellplates with astrocytes onto the corresponding trans-well inserts. Co-cultures were initiated by combining the inserts and the 6-well plates; 48h later RNA was collected from GSCs. RNA was collected from three biological replicates was processed and subjected to microarray analysis on Human Genome U133A 2.0 chips. NSC11: glioblastoma stem-like cells isolated from human glioblastoma surgical specimen patient 1 GBAM1: glioblastoma stem-like cells isolated from human glioblastoma surgical specimen patient 2

INSTRUMENT(S): [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array

SUBMITTER: Barbara Helen Rath  

PROVIDER: GSE63037 | GEO | 2014-11-07



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Coculture with astrocytes reduces the radiosensitivity of glioblastoma stem-like cells and identifies additional targets for radiosensitization.

Rath Barbara H BH   Wahba Amy A   Camphausen Kevin K   Tofilon Philip J PJ  

Cancer medicine 20151030 11

Toward developing a model system for investigating the role of the microenvironment in the radioresistance of glioblastoma (GBM), human glioblastoma stem-like cells (GSCs) were grown in coculture with human astrocytes. Using a trans-well assay, survival analyses showed that astrocytes significantly decreased the radiosensitivity of GSCs compared to standard culture conditions. In addition, when irradiated in coculture, the initial level of radiation-induced γH2AX foci in GSCs was reduced and foc  ...[more]

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