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Induction of ER stress in HCT116 colon cancer cells

ABSTRACT: To investigate the role of p53 and DICER in the induction of ER stress, wildtype, p53 knockout or DICER mutant HCT116 colon cancer cells were treated with the ER stress inducers tunicamycin or brefeldin A for 24 hours. Microarray analysis was used to determine changes in gene expression associated with the induction of ER stress, and to compare this induction in wildtype, p53 knockout or DICER mutant HCT116 colon cancer cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE63252 | GEO | 2015/04/01

SECONDARY ACCESSION(S): PRJNA267109

REPOSITORIES: GEO

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Induction of ER stress in HCT116 colon cancer cells

Project description:To investigate the role of p53 and DICER in the induction of ER stress, wildtype, p53 knockout or DICER mutant HCT116 colon cancer cells were treated with the ER stress inducers tunicamycin or brefeldin A for 24 hours. Microarray analysis was used to determine changes in gene expression associated with the induction of ER stress, and to compare this induction in wildtype, p53 knockout or DICER mutant HCT116 colon cancer cells Triplicate samples of HCT116 wildtype, HCT116 p53 knockout and HCT116 DICEREX5/EX5 cells were treated with with 0.5 mg/ml of BFA or 2 mg/ml of Tm for 24 h. Following treatment, cells were harvested and lysed in TRIzol reagent and RNA was extracted. Microarray analysis was carried out using Affymetrix HG-U133_Plus-2 arrays.

2015-04-01 | E-GEOD-63252 | biostudies-arrayexpress

Homo sapiens

Project description:Induction of ER stress in HCT116 colon cancer cells

| PRJNA267109 | ENA

E-GEOD-63252

Project description:Induction of ER stress in HCT116 colon cancer cells

2015-08-07 | E-GEOD-63252 | ExpressionAtlas

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Gene expression changes in human colorectal cell line HCT116 DICER Exon5 knockout cells

Project description:The experiment was to study the gene expression changes in human colorectal cell HCT116 DICER Exon5 knockout cells comparing to that in parental HCT116 cellls. Keywords: Cell type comparison

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CRISPR knockout screening data and RNAsequencing in LINE-1(+) RPE cells

Project description:CRISPR knockout screens were performed in RPE cells with or without wildtype p53 function to determine the genetic fitness interactions that occur upon induction of LINE-1 protein expression. We also performed RNAseq analysis in wildtype p53 RPE cells expressing LINE-1 or Luciferase control to determine transcriptomic changes that occur upon LINE-1 expression.

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RNA-seq of MV4;11 cells treated with Methyl Bestatin or Brefeldin A

Project description:We report the application of RNA-seq for profiling gene transcription upon treatment of methyl bestatin (MeBs, aminopeptidase inhibitor) or brefeldin A (BFA, ER-to-Golgi transport inhibitor), compared to DMSO control. We found MeBs and BFA similarly upregulated genes pertinent to proteotoxic stress.

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Target suppression effect by methylated miRNA

Project description:To confirme the difference of target supression effect, we transfected non-methylated micro RNA or methylated microRNA in DICER knockout HCT116 cell.

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Transcription profiling of human colorectal cell line HCT116 DICER Exon5 knockout cells

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Genomewide analysis of the human p53 transcriptional network unveils a lncRNA tumor suppressor signature (RNA-seq)

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