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ICLIP seq reveals distribution of genome-wide SR protein-RNA binding

ABSTRACT: We identified the precise genome-wide binding sites for all SR proteins, using iCLIP-seq

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE63262 | GEO | 2014/11/23

SECONDARY ACCESSION(S): PRJNA268197

REPOSITORIES: GEO

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iCLIP seq reveals distribution of genome-wide SR protein-RNA binding

Project description:We identified the precise genome-wide binding sites for all SR proteins, using iCLIP-seq SR proteins were encoded on stable transgenes, transfected in S2 cells, FLAG-tag immunopurified, and the bound RNA purified and subjected to RNA-seq. The resulting reads (CLIP tags) were aligned to the Drosophila genome and generated 38,695-5,900,000 unique CLIP tags for each SR-protein replicate.

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