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Separated and combined effects of the over-expression of a plant defensin and of a 20 mM Zn treatment on yeast.

ABSTRACT: The expression of plant defensins in Saccharomyces cerevisiae has been shown to increase tolerance to Zn of yeast (Mirouze et al., 2006; Shahzad et al., 2013). The present transcriptomic approach has been conducted to uncover the mechanism underlying this phenotype. WT yeast was compared to yeast expressing the AhPFF1.1b plant defensin when grown in both control medium and medium supplemented with 20 mM ZnSO4.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE63395 | GEO | 2015/03/31

SECONDARY ACCESSION(S): PRJNA267661

REPOSITORIES: GEO

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Separated and combined effects of the over-expression of a plant defensin and of a 20 mM Zn treatment on yeast.

Project description:The expression of plant defensins in Saccharomyces cerevisiae has been shown to increase tolerance to Zn of yeast (Mirouze et al., 2006; Shahzad et al., 2013). The present transcriptomic approach has been conducted to uncover the mechanism underlying this phenotype. WT yeast was compared to yeast expressing the AhPFF1.1b plant defensin when grown in both control medium and medium supplemented with 20 mM ZnSO4. 4 conditions with 2 biological replicates compared: control yeast (BY4741) grown on control medium, control yeast grown in the presence of 20 mM ZnSO4, yeast expressing the AhPDF1.1b defensin grown in control medium, yeast expressing the AhPDF1.1b defensin grown in the presence of 20 mM ZnSO4

2015-03-31 | E-GEOD-63395 | biostudies-arrayexpress

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Expression data from root tissues of 35S:AtHMA4 and wild-type tomato plants grown in the presence of Zn

Project description:Ectopic expression of AtHMA4 in tomato modified Zn accumulation in a Zn-supply dependent manner. It suggests that HMA4-expression under a range of Zn-supply differentially modifies the expression of endogens, which contributes to different Zn-related phenotypes. To identify genes differentially regulated in 35S:AtHMA4-expressing and wild-type tomato, the transcription profiles were compared between roots of transgenic and WT-plants grown in the presence of 5 µM Zn. An effort was undertaken to recognize tissue specific alterations underlying the difference in Zn root:shoot distribution between tested plant lines and LCM technique was used to isolate epidermis+cortex and stele from roots.Understanding the interplay between the transgene and the endogens is crucial for planning genetic modification for Zn-biofortification purpose. 10-day old tomato plant (35S:AtHMA4 expressing and WT) were subjected to 5 µM Zn (added as ZnSo4) for 15 days. Three independent experiments were performed. At the end of each experiment 2.0 cm long root fragments from six plants (excluding 1.5 cm long apical fragments) were pooled, embedded in NEG-50 Frozen Section Medium (Thermo Scientific), and frozen in liquid nitrogen. Total RNA isolated from three batches of roots was amplified and used for three independent microarray analysis.

2015-05-08 | E-GEOD-68639 | biostudies-arrayexpress

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Project description:Arantes et al, 2021

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Project description:To investigate the effect of the Zn ionophore, PBT2, on the transcriptomic response of Fusobacterium nucleatum ATCC 25586, RNA was extracted from bacterial samples which had been treated as follows: Untreated (0.006% DMSO v/v), DMSO-Zn treated (0.006% DMSO v/v + 200 uM ZnSO4), or PBT2-Zn treated (0.125 ug/mL PBT2 + 200 uM ZnSO4) (DMSO was the vehicle control). RNA samples were collected at 0h, 0.5h, and 1h post challenge in biological triplicate and sequenced using Illumina HiSeq platform. We mapped sequences to the reference genome F. nucleatum subsp. nucleatum ATCC 25586 and performed DEseq2 analysis to determine differentially expressed genes across time and treatment.

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