Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq analysis of contextual fear conditioning

ABSTRACT: This dataset constitutes the first RNA-seq study of gene expression following contextual fear conditioning in the mouse hippocampus.

ORGANISM(S): Mus musculus

PROVIDER: GSE63412 | GEO | 2015/07/22

SECONDARY ACCESSION(S): PRJNA267703

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Sono-Seq analysis of contextual fear conditioning

Project description:This dataset constitutes the first Sono-Seq study of chromatin accessibility following contextual fear conditioning in the mouse hippocampus.

2017-11-20 | GSE98317 | GEO

RNA-seq analysis of contextual fear conditioning

Project description:This dataset constitutes the first RNA-seq study of gene expression following contextual fear conditioning in the mouse hippocampus. 15 total samples were analyzed, including animals trained in a contexual conditioning paradigm (FC) and controls. Tissue was collected at 30 minutes (FC), as well as 30 minutes after testing for retrieval of the memory (RT). Testing was performed at 24 hours after training over a 5-minute interval. Animals that were handled but not trained were dissected at the same time of day to control for variations due to circadian rhythms (CC3). The protocol was repeated over the course of 5 days to obtain 9 animals (2 hippocampi) per group, so that 5 independent FC experiments were represented in each time point and all animals for each group were dissected at the exactly the same time of day.

2015-07-22 | E-GEOD-63412 | biostudies-arrayexpress

Contextual fear conditioning induced transcriptional changes in hippocampal CA1 neurons

Project description:The goal of our study was to assess whether the experience can regulate specific lncRNAs within the hippocampus and their role in associative memory. To address this, we carried out unbiased analyses of gene expression in CA1-hippocampal neurons to identify lncRNA changes induced by contextual fear conditioning (CFC).

2023-08-07 | GSE214838 | GEO

Genome-wide profilings of transcriptome and translatome in mouse hippocampi after contextual fear conditioning

Project description:Memory stabilization after learning requires transcriptional and translational regulations in the brain, yet the temporal molecular changes following learning have not been explored at the genomic scale. We here employed ribosome profiling and RNA sequencing to quantify the translational status and transcript levels in mouse hippocampus following contextual fear conditioning. We identified 104 genes that are dynamically regulated. Intriguingly, our analysis revealed novel repressive regulations in the hippocampus: translational suppression of ribosomal protein-coding genes at basal state; learning-induced early translational repression of specific genes; and late persistent suppression of a subset of genes via inhibition of ESR1/ERα signaling. Further behavioral analyses revealed that Nrsn1, one of the newly identified genes undergoing rapid translational repression, can act as a memory suppressor gene. This study unveils the yet unappreciated importance of gene repression mechanisms in memory formation.

2015-10-07 | GSE72064 | GEO

Microarray dataset of gene transcription in mouse microglia and peripheral monocytes in contextual fear conditioning

Project description:Using Illumina MouseWG-6v2 microarrays, we investigated the gene transcription changes in microglia and peripheral monocytes after contextual fear conditioning of C57BL/6J mice. Mice were trained with or without a single minimized footshock stimulation (0-s or 2-s, 0.4 mA) and re-exposed to the training context without footshock for three different durations 24 h later: 0 min (FS0), 3 min (FS3), or 30 min (FS30). Whole brain microglia and peripheral monocytes were prepared 24 h after re-exposure using a neural tissue dissociation kit, including non-footshock controls for two re-exposure durations (Con3 and Con30). The data can be valuable for researchers interested in glial cells and neurotransmission studies and are related to the research article “Contextual fear conditioning regulates synapse-related gene transcription in mouse microglia”.

2022-10-28 | E-MTAB-12361 | biostudies-arrayexpress

Time-lapse hippocampal transcriptomic profiling after fear conditioning in Tob WT and KO mice

Project description:Fear conditioning induces immediate changes in the mouse hippocampal transcriptome profile. Here, we performed RNA sequqencing in mice hippocampi at 15 minutes, 1 hour and 3 hours post-fear conditioning in addition to non-conditioned mice. Tob gene has been shown to affect cellular stress and behavior. In order to understand the role of Tob gene in the hippocampal stress and fear machinery, we compared hippocampi of Tob WT and KO mice. This dataset shows the temporal transcriptomic changes in mouse hippocampus induced by fear conditioning. Also, it shows the changes occurred after Tob deletion.

2022-08-16 | GSE186101 | GEO

Gene expression in rat hippocampal CA1 during the extinction of contextual fear memory

Project description:Adult rats (male Lister hooded) were pre-exposed for 3 d for 10 min /d (24 hr interval) to two different novel contexts. On day 4 rats received a single unsignaled scrambled footshock (0.5 mA for 2 s) 2 min into a 3min exposure to one of the two contexts. Rats were either returned to the CS conditioned context (CS+, EXT group) or to the other (CS-, NOR group) context for 10 min, 48h after contextual fear conditioning. Rats were housed in the home cages at all times outside the conditioning and re-exposure components. The novel context chosen to be the conditioned context was counterbalanced across the experimental groups. Rats were killed by CO2 inhalation 2 hours after CS- or CS+ exposure. CA1-enriched dorsal hippocampus was immediately dissected on ice before rapid freezing and storage at -80oC. The CS+ group represents rats that underwent extinction of contextual fear memory.

2015-09-25 | GSE66151 | GEO

Npas4 CUT&Tag in amygdala tissue after discriminative fear conditioning

Project description:Npas4 CUT&Tag dataset, Adult male WT C57BL/6J mice underwent discriminative fear conditioning and immediately injected saline. 90 minutes after fear conditioning, The amygdala tissue was extracted for further experiment. Npas4 CUT&Tag was performed to elucidate possible downstream targets which contributes regulation of fear expression during fear retreival.

2023-07-05 | GSE194069 | GEO

Gene-expression changes in neurons active during contextual fear conditioning compared to controls left in the homecage

Project description:This experiment uses a transgenic mouse that allows for activity-induced expression of an HA-tagged ribosome in an inducible and cre-dependent manner. This allowed us to assess long-lasting gene-expression changes in neurons active during contextual fear conditioning. Previous studies have used similar systems to look a transient changes in gene expression in these neurons, but we developed a system to look at long lasting changes. The workflow includes tissue dissection of the prefrontal cortex, tissue homogenization followed by an immunoprecipitation of the tagged ribosomes using antibodies against HA. RNA bound to the ribosomes is then purified, a cDNA library is prepared and RNAseq was performed. The experimental group was fear conditioned and the control group was left in their homecage for induction of HA- expression in active neurons.

2020-03-27 | E-MTAB-7836 | biostudies-arrayexpress

Adult Female Rat Hippocampal Tissue: Naïve vs. Contextual Fear Conditioned (Trained)[Experiment 2]

Project description:naïve and trained rats 24 hours after exposure to the contextual fear conditioning paradigm. Biological replicates: 4 naïve and 4 trained animals, independently conditioned and tissues were independently isolated. One replicate per array.

2017-03-08 | GSE84262 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data