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Lentiviral expression of MiR-31 in CD8 T-cells.

ABSTRACT: Purpose: identify genes regulated by expression of miR-31 in primary mouse CD8 T-cells by exogenously expressing pre-miR-31 from the Plko.3g lentiviral vector. Cells infected with empty Plko.3g vectors were used as controls for infection.

ORGANISM(S): Mus musculus

PROVIDER: GSE63549 | GEO | 2015/11/01

SECONDARY ACCESSION(S): PRJNA268189

REPOSITORIES: GEO

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Lentiviral expression of MiR-31 in CD8 T-cells.

Project description:Purpose: identify genes regulated by expression of miR-31 in primary mouse CD8 T-cells by exogenously expressing pre-miR-31 from the Plko.3g lentiviral vector. Cells infected with empty Plko.3g vectors were used as controls for infection. Control vector and miR-31 transduced CD8 T-cells with 4 independent biological replicates/condition

2015-11-01 | E-GEOD-63549 | biostudies-arrayexpress

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Project description:Transcriptomics analyses in these Zn-deficient rats revealed the molecular basis of ESCC abrogation by miR-31 knockout: Egln3, a negative regulator of NF-FB, was shown to be a direct miR-31 target; miR-31 inhibition/deletion resulted in suppression of miR-31-associated-EGLN3-NF-KB controlled inflammatory pathways.

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