Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profiling of the AD11 anti-NGF transgenic mouse model of neurodegeneration at 1, 3, 6, 15 months of age

ABSTRACT: We characterized the gene expression profile of brain regions at different stages of the Alzheimer’s like neurodegeneration in the anti-NGF AD11 trangenic mouse model. Total RNA was extracted from hippocampus, cortex and basal forebrain of postnatal day 30 (P30, 1 month), P90 (3 months), P180 (6 months) and at 15 months of age. Expression profiles were studied by Agilent microarray analysis, followed by qRT-PCR validation of significant candidates. Wide changes in gene expression profiles occur already at the presymptomatic stage P30, with the most significantly affected clusters of mRNAs are linked to Inflammation and Immune Response.

ORGANISM(S): Mus musculus

PROVIDER: GSE63617 | GEO | 2015/02/26

SECONDARY ACCESSION(S): PRJNA268478

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Gene expression profiling of the AD11 anti-NGF transgenic mouse model of neurodegeneration at 1, 3, 6, 15 months of age

Project description:We characterized the gene expression profile of brain regions at different stages of the AlzheimerM-bM-^@M-^Ys like neurodegeneration in the anti-NGF AD11 trangenic mouse model. Total RNA was extracted from hippocampus, cortex and basal forebrain of postnatal day 30 (P30, 1 month), P90 (3 months), P180 (6 months) and at 15 months of age. Expression profiles were studied by Agilent microarray analysis, followed by qRT-PCR validation of significant candidates. Wide changes in gene expression profiles occur already at the presymptomatic stage P30, with the most significantly affected clusters of mRNAs are linked to Inflammation and Immune Response. AD11 mice were compared to the trangenic VH controls. A total of 60 female AD11 mice and 59 female control VH (5 per group) mice were used for this study: 5 AD11 and 4-5 control VH mice for each time point (1,3,6,15 months of age) and brain area (HP, CTX, BFB).

2015-02-26 | E-GEOD-63617 | biostudies-arrayexpress

Comparison of murine colonic mucosal DNA from postnatal day 90 (P90) to postnatal day 30 (P30) by MSAM

Project description:DNA methylation profiling of colonic mucosal DNA between P90 and P30 mice. 0.5ug of DNA was serially digested with SmaI and XmaI followed by an adaptor ligation and adaptor mediated PCR amplification Two independent P90 to P30 comparisons were performed as follows. Samples were labelled with Cy3 (P30) and Cy5 (P90) and two independent P90 to P30 comparisons were done on a 2x105k methylation specific amplification microarray (MSAM) containing 90,535 probes, covering 77% of the 31,019 SmaI intervals between 200 bp and 2 kb in the mouse genome (average 3.8 probes per interval)

2010-05-22 | E-GEOD-18031 | biostudies-arrayexpress

Comparison of murine colonic mucosal gene expression between postanatal day 90 (P90) to postnatal day 30 (P30)

Project description:Comparison of murine colonic mucosal gene expression between postanatal day 90 (P90) to postnatal day 30 (P30) by whole genomic expression microarray. Gene expression profiling of colonic mucosal DNA between P90 and P30 mice. Agilent Technologies two-color labelling kit and genomic hybridization protocol was utilized.

2010-01-30 | GSE19506 | GEO

RNA changes in hippocampus of transgenic murine model of tauopathy (rTg4510 mice) compared to controls at asymptomatic stage (2 months) of neurodegeneration as determined by mRNA deep sequencing.

Project description:The overall goal of the project is to assess the mRNA alterations in tauopathy conditions that occur at the presymptomatic stage of neurodegeneration. The animal model selected for this study is a well characterized tauopathy animal model, known as rTg4510 murine model. rTg4510 (tau) is a transgenic mouse model of human tauopathy expressing human tau containing the P301L mutation that is associated with familial frontotemporal dementia and parkinsonism linked to chromosome 17. The expression of P301L tau driven by CaMKII promoter peaks around two month-of-age and mainly in the forebrain. 2-month of age is considered the presymptomatic stage of neurodegeneration. The global expression of mRNAs was evaluated by mRNA deep sequencing in the hippocampus of tau mice at 2 months of age. The levels of several mRNAs were altered in the hippocampus of tau mice at 2 months. Many important candidates were further evaluated for their molecular and functional contributions to the process of neurodegeneration.

2018-07-24 | GSE107183 | GEO

NGF Treatment of PC12 Cells

Project description:Expression profiling of PC12 cells stably transfected with GFP, GFP-SH2-Bb, or GFP-SH2-Bb(R555E), treated with or without NGF for 6h Keywords: response to NGF

2007-03-27 | GSE4557 | GEO

Comparison of murine colonic mucosal DNA from postnatal day 90 (P90) to postnatal day 30 (P30) by MSAM

2009-09-30 | GSE18031 | GEO

miRNA expression profile in PC12 cells under NGF treatment and deprivation

Project description:miRNA profiling of PC12 rat pheochromocytoma cells during NGF-induced differentiation and apoptosis of differentiated cells after 24h of NGF deprivation. miRNA expression in differentiating and deprivated cells was compared to untreated and terminally differentiated PC12 cells respectivelly. Five-condition experiment, NGF-treated cells (1h, 3h, 6h, 24h, 240h) vs. untreated. One-condition experiment, NGF-deprivated vs. differentiated cells.

2013-05-11 | E-GEOD-46827 | biostudies-arrayexpress

Proteome analysis of NGF stimulated PC12 cells

Project description:We used quantitative mass spectrometry-based proteomics to unravel global nerve growth factor (NGF)-induced changes in protein abundance using the rat PC12 cell line. Cells were stimulated with NGF for 0, 24 and 48 h in a triple SILAC setup (Light: Lys0,Arg0; Medium: Lys4,Arg6; and Heavy: Lys8,Arg10). All experiments were performed as biological replicates.

2015-05-06 | PXD001478 | Pride

Transcriptional programs controlled by NGF signaling in developing mouse dorsal root ganglia neurons

Project description:During embryogenesis, nociceptive sensory neurons of the dorsal root ganglia depend intimately on Nerve Growth Factor (NGF) for neuronal survival, maturation and target innervation. NGF is a secreted molecular signal synthesized by neuronal target tissues. In developing nociceptors, NGF engages the receptor tyrosine kinase TrkA to activate a gene transcriptional program involving the regulation of hundreds of transcripts. To identify NGF-dependent genes in developing mouse nociceptors, we have designed and performed two separate microarray screens to compare gene expression profiles of DRG neurons either with or lacking NGF signaling.

2013-11-01 | GSE25611 | GEO

miRNA expression profile in PC12 cells under NGF treatment and deprivation

2013-05-11 | GSE46827 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data