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Tbr2 and Neurog2 occupancy and transcriptional profiling of control and Tbr2 knockout E14.5 cerebral cortices

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE63621 | GEO | 2016/09/08

SECONDARY ACCESSION(S): PRJNA268481

REPOSITORIES: GEO

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Similar Datasets

Tbr2 and Neurog2 occupancy and transcriptional profiling of control and Tbr2 knockout E14.5 cerebral cortices

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-09-08 | E-GEOD-63621 | biostudies-arrayexpress

Tbr2 and Neurog2 transcription factor binding sites in E14.5 WT cerebral cortices

Project description:To date, speculations on the molecular roles of Tbr2 transcription factor during specification, maintenance and differentiation of cortical specific Intermediate Neural Progenitors are coming from the analysis of loss- and gain-of-function experiments. However since its capacity to bind the DNA to extert its function we did Chromatin Immuno-Precipitation followed by deep sequencing to profile its target on the genome. We performed this approach directly in vivo to respect the physiological and peculiar enviroment of its action during cortical development. Moreover we did the same approach for Neurogenin 2 proneural gene. Interestingly the vast majority of the Neurog2 peaks are in common with Tbr2 dataset suggesting a co-operation between the two factors confirmed by biochemical and functional assays. Finally we compared Tbr2 dataset with the DNA regions bound by the H3K27me3 histone demethylase Jmjd3 (NCBI:Kdm6b) obtained by others. Interestingly we were able to find regions in common linked to important genes for neuronal differentiation.

2016-09-08 | GSE63620 | GEO

Tbr2 and Neurog2 transcription factor binding sites in E14.5 WT cerebral cortices

2016-09-08 | E-GEOD-63620 | biostudies-arrayexpress

Transcriptional profiling of E14.5 control and Tbr2 fl/fl;Foxg1::Cre cortices

Project description:The abscence of TBR2 gene in human leads to microcephaly. This condition is mimicked by the specific ablation of the murine gene in developing cerebral cortex. Herein we compared gene expression in control and Tbr2 cKO in E14.5 cerebral cortices. This approach represents a useful tool to identify the molecular mechanisms at the basis of the phenotype.

2016-09-08 | GSE63619 | GEO

Transcriptional profiling of E14.5 control and Tbr2 fl/fl;Foxg1::Cre cortices

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Gene expression in basal progenitors in the cerebral cortex of Tbr2 conditional knockout mice

Project description:During cortical development neurons are generated from basal progenitors (BPs) which specifically express the transcription factor Tbr2. We used fluorescent-activated cell sorting (FACS) to isolate BPs from Tbr2-conditional knockout mice brain at early (E13) and late (E16) stages of cortical neurogenesis and determined mRNA expression profiles using microarray (Illumina MouseWG-6 v2). Role of transcription factor Tbr2 in basal progenitors during corticogenesis.

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Prenatal ?-catenin/Brn2/Tbr2 transcriptional cascade regulates adult social and stereotypic behaviors.

Project description: Not available

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Gene expression in Tbr2 lineage neurons in the murine cerebral cortex

Project description:During cortical development neurons are generated from basal progenitors (BPs) which specifically express the transcription factor Tbr2. We used fluorescent-activated cell sorting (FACS) to isolate Tbr2-lineage neurons (GFP+) from mice brain at postnatal day 2 (P2) and determined mRNA expression profiles using microarray (Illumina MouseWG-6 v2). Role of transcription factor Tbr2 in neurodevelopment.

2019-10-18 | GSE121470 | GEO

Parcellation of the primary cerebral cortices based on local connectivity profiles.

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Expression data from cerebral cortices of bacTRAP transgenic mice

Project description:We used microarrays of eight different cell types in cortex to conduct specificity index analysis for detailed cell type specific molecular profile. Cerebral cortices of adult bacTRAP transgenic mice were dissected out and subjected to polysomal immunoprecipitation with GFP antibody in accordance with the established protocol (Heiman et al. Cell 2008). Enriched RNAs for each cell type were extracted from corresponding bacTRAP mice and subjected to two cycle RNA amplification and biotin labeling following quality control. Biotin labeled RNAs were hybridized on Mouse Genome 430 2.0 microarray chips and the expression intensity of all 40,000 probesets were analyzed further.

2015-06-30 | E-GEOD-69340 | biostudies-arrayexpress

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