Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A New Type of Human iPS Cells: Human Induced Hepatic Lineage-Oriented Stem Cells

ABSTRACT: Human induced hepatic lineage-oriented stem cells (hiHSCs) can be established as a new type of human induced pluripotent stem cells (hiPSCs) with only some modifications of their culture conditions in both generation and self-renewal. hiHSCs were generated and expanded at a very high density under non-typical coculture with feeder cells in a chemically defined hiPSC medium . In detail, hiHSCs were generated from human non-hepatic cells by gene transfer with pantropic retrovirus vectors carrying the three transcription factor genes OCT3/4, SOX2, and KLF4. They were expanded at a very high density under non-typical coculture with mouse embryonic fibroblasts (MEF) in a chemically defined mTeSR1 medium on gelatin-coated dishes. Self-renewing hiHSCs expressed markers of both human embryonic stem cells (hESCs) and hepatocytes. The potential hepatic specification of hiHSCs was defined by their expression profiles of hepatic markers under a self-renewal culture.

ORGANISM(S): Homo sapiens

PROVIDER: GSE63844 | GEO | 2015/03/13

SECONDARY ACCESSION(S): PRJNA269247

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Mus musculus

Project description:Self-renewal of hepatoblasts under chemically defined conditions by reiterative growth factor and chemical screening

| PRJNA260284 | ENA

Self-renewal of hepatoblasts under chemically defined conditions by reiterative growth factor and chemical screening

Project description:Throught an reiterative growth factor and chemical screening, we defined a small molecule and growth factor cocktail, including EGF, glycogen synthase kinase 3 inhibitor (CHIR99021), transforming growth factor β receptor inhibitor (e.g. E-616452), lysophosphatidic acid and sphingosine 1-phosphate, that can sustain long-term self-renewal of murine hepatoblasts under chemically defined conditions. The expandable hepatoblasts (eHBs) by this small molecule and growth factor cocktail expressed a set of genes typical of liver progenitor cells and liver development, and retained the ability to respond to liver developmental cues and produce functional hepatocytes and form bile duct-like structures. Moreover, both early- and late-passage eHBs demonstrated a similar transcriptome profile. Microarray analysis also confirmed that the gene expression of cultured cells resembled hepatoblasts.

2014-12-29 | GSE61119 | GEO

Niche-Independent Expansion and Controlled Differentiation of Intestinal Stem Cells in vitro

Project description:While stem cells at the bottom of intestinal crypts have been isolated and expanded in vitro in the form of organoids, the self-renewal and differentiation of Lgr5+ stem cells in typical culture conditions cannot be easily controlled. Here we show that two small molecules, CHIR99021 and Valproic Acid, synergistica

2014-04-02 | E-MTAB-1978 | biostudies-arrayexpress

GDNF-regulated gene expression in cultured mouse spermatogonial stem cells

2006-05-11 | GSE4799 | GEO

Transcription profiling of spermatogonial stem cells established from 6 day old male mice to identify GDNF regulated genes by comparing cells in the presence, absence and timed replacement of GDNF

Project description:GDNF-regulated gene expression was studied in cultures of actively self-renewing spermatogonial stem cells established from 6 day old male mice. GDNF is the essential growth factor regulating mouse spermatogonial stem cell self-renewal. Using a serum-free chemically defined culture system that supports mouse spermatogonial stem cell self-renewal for extended periods of time, GDNF-regulated genes were identified using microarray profiling. Experiment Overall Design: Established cultures of highly enriched self-renewing spermatogonial stem cells were subjeted to withrawal of GDNF and GFRalpha1 for 18-hr followed by replacement of the growth factors for 2, 4, and 8-hr. Gene expression was studied using microarray profiling prior to withdrawal, after withdrawal and at each time-point of GDNF/GFRalpha1 replacement.

2007-11-24 | E-GEOD-4799 | biostudies-arrayexpress

FGF-WNT omic correlation project

Project description:Both FGF and WNT pathways play important roles in embryonic development, stem cell self-renewal and are frequently deregulated in breast cancer. To study the cooperation between FGF and WNT signaling, we have generated a mouse model, MMTV-WNT1/MMTV-iFGFR1 (WNT/iR1), in which we could chemically overactivate iFGFR1 in a ligand-independent manner.

2017-11-22 | MSV000081730 | MassIVE

Human umbilical cord blood-derived endothelial colony forming cells under serum-free conditions

Project description:Derivation and expansion of human umbilical cord blood-derived endothelial colony forming cells under serum-free conditions - a transcriptome analysis. Endothelial colony forming cells (ECFCs) were isolated from term umbilical cord blood units. ECFCs were expanded under standard, fetal bovine serum (FBS) containing endothelial medium, or transferred to chemically defined endothelial media without FBS. Microarray expression profiling was applied to compare the transcriptome profiles in FBS-containing versus FBS-free culture.

2010-06-29 | GSE20504 | GEO

Human umbilical cord blood-derived endothelial colony forming cells under serum-free conditions

2010-07-29 | E-GEOD-20504 | biostudies-arrayexpress

Homo sapiens

Project description:Analysis of SOX2 downregulation under self-renewal conditions.

| PRJNA281470 | ENA

Comparison of liver mRNA expression between Control AA and Low AA diet fed mice

Project description:The goal of the experiment was to examine hepatic gene expression differences induced by a low protein high carbohydrate diet. We used chemically defined diets based on naturally defined diets first published and completely described in Fontana et al., 2016, Cell Reports.

2019-01-17 | GSE115683 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data