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V. vinifera 'Cabernet sauvignon' and V. aestivalis 'Norton' innoculated with Erysiphe necator conidiospores

ABSTRACT: we analyzed pathogen-induced changes in the transcriptome of Vitis vinifera ‘Cabernet sauvignon’ and Vitis aestivalis ‘Norton’ by conducting a large-scale study to measure transcript abundance at 0, 4, 8, 12, 24, and 48 hours post-treatment in conidiospore- and mock-inoculated leaves using Affymetrix GeneChip Vitis vinifera Genome Array Keywords: time course

ORGANISM(S): Vitis aestivalis Vitis vinifera

PROVIDER: GSE6404 | GEO | 2007/11/29

SECONDARY ACCESSION(S): PRJNA99537

REPOSITORIES: GEO

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Transcription profiling of V. vinifera Cabernet sauvignon and V. aestivalis Norton innoculated with Erysiphe necator conidiospores

Project description:we analyzed pathogen-induced changes in the transcriptome of Vitis vinifera âCabernet sauvignonâ and Vitis aestivalis âNortonâ by conducting a large-scale study to measure transcript abundance at 0, 4, 8, 12, 24, and 48 hours post-treatment in conidiospore- and mock-inoculated leaves using Affymetrix GeneChip Vitis vinifera Genome Array Experiment Overall Design: sixty plants were grown in each of two identical growth chamber labeled as Inoculation and Mock-inoculation under same conditions. Pairs of vines of same size were labeled, one with an âIâ, the other with a âMâ prefix for âinoculatedâ and âmockâ. The vines in each pair received the same ID number. This resulted in balanced groups for inoculated and mock-inoculated vines. âIâ and âMâ vines of a pair were placed in corresponding locations on the bench of the two growth chambers. The locations of the vines were determined by randomizing their ID numbers. Thus, the âIâ and âMâ groups were balanced according to their size and to their locations within the growth chamber. All vines were positioned at the same height from the light source. The locations of the individual plants within the growth chamber were recorded. At a time point, 10 randomly selected pairs of plants were sampled. (For example, at 8 hours post-inoculation, vines I-3, I-17, I -23, ..etc., and vines M-3, M-17, M-23, ...etc. were sampled from the âIâ and the âMâ growth chambers, respectively). The 10 corresponding leaves were combined into a single sample. At the next time point, another set of randomly selected 10 pairs were sampled from among the remaining plants, and so on. This random selection was independent from the random selection implemented to determine the location of the vines in the growth chambers

2008-06-14 | E-GEOD-6404 | biostudies-arrayexpress

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Grape skin transcriptome to reveal genes related with resveratrol accumulation

Project description:Methods:transcriptomes of the different development stages of Vitis vinifera cv. Cabernet Sauvignon and Vitis quinquangularis accession Danfeng-2 were analyzed using Illumina Hiseq 2500. The sequence reads that passed quality filters were analyzed: TopHat followed by Cufflinks. mRNA profiles of different development stages of Vitis vinifera cv. Cabernet Sauvignon and Vitis quinquangularis accession Danfeng-2 were generated by deep sequencing, in triplicate, using Illumina Hiseq 2500.

2015-12-23 | E-GEOD-76256 | biostudies-arrayexpress

V. vinifera 'Cabernet sauvignon' and V. aestivalis 'Norton' innoculated with Erysiphe necator conidiospores

Project description:V. vinifera 'Cabernet sauvignon' and V. aestivalis 'Norton' innoculated with Erysiphe necator conidiospores

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Vitis vinifera cultivar:Cabernet Sauvignon

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High temperature effect on Cabernet Sauvignon berries

Project description:The experiment was conducted using four 11-year-old potted grapevines of V. vinifera L. cv. Cabernet Sauvignon grafted on SO4 (Selection Oppenheim No. 4) grown in a phytotron. Vines were trained on a Guyot trellising system and each vine carried 4-10 clusters of grapes. The experiment started approximately 1 week before veraison, when berry softening started, and continued to fruit maturity. The two temperature regimes consisted of a high day (06.00–20.00 h) temperature (max.35C) and a control (max. 25C). Under both conditions, the night-time (20.00–06.00 h) temperature was 20C. PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Kentaro Mori. The equivalent experiment is VV9 at PLEXdb

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Root transcriptome comparison between grapevines Cabernet Sauvignon, 140 Ruggeri and K51-40 (+/- salt stress)

Project description:Salt tolerance in grapevine is associated with the exclusion of chloride ions (Cl–) from the shoot. The rate-limiting step for this process has been identified as the passage of Cl– between the root symplast and the xylem apoplast through membrane integral proteins. To identify candidate genes for these proteins we used a custom microarray to compare the root transcriptomes of three grapevine varieties (Vitis spp.) that differ in their capacity to exclude Cl– from shoots. When challenged with 50 mM Cl– there were transcriptional responses that differed across the rootstocks 140 Ruggeri (a good Cl– excluder) and K51-40 (a poor Cl– excluder), and Cabernet Sauvignon (an intermediate Cl– excluder and Vitis vinifera control). The magnitude of these salt-induced changes correlated with the amount of Cl– accumulated in shoots. Abiotic-stress responsive transcripts (e.g. heat shock proteins) were induced in 140 Ruggeri. Respiratory transcripts were repressed in Cabernet Sauvignon. Expression of hypersensitive response and ROS scavenging transcripts were altered in the sensitive K51-40. Despite these differences, no obvious candidate Cl– transporters were identified from the salt treatment. In contrast, under control conditions where differences in shoot Cl– exclusion between rootstocks were still significant, we identified a number of genes encoding putative ion channels including VvSLAH3, VvALMT1, and possible regulators of these proteins such as VvSnRK2.6 and calcium dependent protein kinases (CDPK) as being differentially expressed between rootstocks. Members of the low affinity nitrate transporter (NRT1), and chloride channel (CLC) families were also identified. We propose these as useful candidates for further study within breeding programs aimed at improving plant salt tolerance in grapevine and other crops.

2014-09-30 | GSE57770 | GEO

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