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Expression analysis of Human esophagus cancer cell line TE-1 transfected with PCDNA3.1 and PCDNA3.1-Pax2

ABSTRACT: Investigation of whole genome gene expression level changes of TE-1 transfected with PCDNA3.1-Pax2 ,compared to TE-1 transfected with PCDNA3.1.

ORGANISM(S): Homo sapiens

PROVIDER: GSE64093 | GEO | 2014/12/12

SECONDARY ACCESSION(S): PRJNA270101

REPOSITORIES: GEO

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Expression analysis of Human esophagus cancer cell line TE-1 transfected with PCDNA3.1 and PCDNA3.1-Pax2

Project description:Investigation of whole genome gene expression level changes of TE-1 transfected with PCDNA3.1-Pax2 ,compared to TE-1 transfected with PCDNA3.1. Compare PAX2 activated genes by analyzing mRNA profilings between pcDNA3.1 and pcDNA3.1-PAX2- transfected TE-1 cells.

2014-12-12 | E-GEOD-64093 | biostudies-arrayexpress

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Project description:Expression analysis of Human esophagus cancer cell line TE-1 transfected with PCDNA3.1 and PCDNA3.1-Pax2

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Human Breast Cancer Cell MCF-7: Control vs. Gli1 Transfected

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Genome-wide maps of TBX1 state in transfected BCPAP cells

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NCI-H1299 cell line transfected with human DENND2D and vector

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Pax2 Dependent Expression in the Intermediate Mesoderm at E11.5

Project description:Heterozygous and homozygous Pax2 E11.5 embryos were collected and the intermediate mesoderm was dissected and dispersed into single cells. The Pax2 positive cells also expressed EGFP, which was knocked into the Pax2 locus. EGFP positive cells were sorted by FACS and RNA isolated. We compared RNA expression levels in EGFP positive cells from Pax2 null and Pax2 heterozygote embryos.

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TE-Array-- A high throughput tool to study transposon transcription

Project description:TE-Array is a custom Agilent microarray that probes for transposable elements in both sense and antisense orientations in humans and mice. TE-Array was used to study expression levels of transposable elements (TE) in a panel of normal mouse tissues. TE-Array comprises of 4 different custom Agilent 4X44K designs. These 4 different designs have probes for human sense orientation, human antisense orientation, mouse sense orientation and mouse antisense orientation transposable elements. These array designs were used to study the transposon abundance profile in 7 different mouse tissue samples, 2 mouse cell lines and 1 transposon transfected versus untransfected human cell line.

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