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Adult mouse stage synchronized testis array

ABSTRACT: WIN 18,446/RA treatment of neonatal male mice was used to synchronize spermatogenesis to 2-3 different stages of the cycle of the seminiferous epithelium in the adult testis

ORGANISM(S): Mus musculus

PROVIDER: GSE64214 | GEO | 2014/12/17

SECONDARY ACCESSION(S): PRJNA270393

REPOSITORIES: GEO

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Adult mouse stage synchronized testis array

Project description:WIN 18,446/RA treatment of neonatal male mice was used to synchronize spermatogenesis to 2-3 different stages of the cycle of the seminiferous epithelium in the adult testis 6 different groups of synchronized adult mouse testis samples (N = 2 per group), each representing only 2-3 stages of the cycle, were collected and analyzed using GeneChip Mouse Gene ST 1.0 Arrays

2014-12-17 | E-GEOD-64214 | biostudies-arrayexpress

Riding the spermatogenic wave: Profiling gene expression within neonatal germ and Sertoli cells during a synchronized initial wave of spermatogenesis

2014-05-01 | GSE54408 | GEO

Riding the spermatogenic wave: Profiling gene expression within neonatal germ and Sertoli cells during a synchronized initial wave of spermatogenesis

Project description:WIN 18,446/RA treatment of neonatal mice was used to synchronize the initial wave of spermatogenesis and identify novel messages expressed within either germ or Sertoli cells as spermatogonia enter meiosis. germ cell-specific (Stra8-cre: RiboTag; or Ngn3-cre:RiboTag) and Sertoli cell-specific (Amh-Cre: RiboTag)

2014-05-01 | E-GEOD-54408 | biostudies-arrayexpress

Leydig cell transcripts from adult mouse stage synchronized testis array

Project description:Leydig cell gene expression across the different stages of the seminiferous epithelium of mice with Synchronized spermatogenesis by WIN18,446/RA treatment combined with the RiboTag methodology Microarray analysis was used to identified Leydig cell-specific transcripts across the different stages of the seminiferous epithelium.

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WIN 18,446 2dpp testis culture

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WIN 18,446 2dpp testis culture

Project description:Analysis of 2dpp whole testes cultured for 24h in the presence of WIN 18,446, a BDAD compound. BDADs (Bis-(dichloroacetyl)-diamines) have been shown previously to inhibit spermatogenesis and function as male contraceptives in many species; however, their mechanism of action has yet to be fully described. Results provide insight into the ability of WIN 18,446 to inhibit retinoic acid synthesis in the murine testis. Testes were removed from 2dpp 129 mice and cultured for 24h in an organ culture mold in the presence of WIN 18,446. Total RNA was extracted and hybridized to Affymetrix Mouse Genome 430 2.0 arrays.

2010-11-25 | E-GEOD-25610 | biostudies-arrayexpress

10x Genonics Drop-seq single-cell RNA-seq of neonatal (P1.5) testis cells from mice treated with DMSO or Retinoic Acid.

Project description:To reveal distinct transcriptomes associated with response of neonatal prospermatogonia to retinoic acid (RA), unselected testis cells from animals treated with RA or DMSO were used for 10x Genomics analysis 12 hours after treatment at P1.

2019-05-01 | GSE117437 | GEO

Thyroid hormone regulated genes in the neonatal testis

Project description:An excess in thyroid hormone during rodent neonatal life causes abnormal prolifration and maturation of testicular cells, leading to reduced testis size and impairments in steroidogenesis, Sertoli cell function, spermatogenesis and fertility. The high expression of type 3 deiodinase in the neonatal testis protects this tissue from premature exposure to thyroid hormones, since this gene (Dio3) function is to degrade thyroid hormones. DIO3-deficient mice (Dio3KO) exhibit a marked reduction in Sertoli cell proliferation and testis size, abnormalities in the reproductive axis and impaired fertility (Martinez et al. 2016, Endocrinology157:1276). To identify genes that are untimely regulated by thyroid hormone in the developing testes, we have performed RNAseq in testis total RNA from wild type and DIO3KO mouse neonates.

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Profiling of long noncoding RNAs and mRNAs in neonatal and adult mouse testis

Project description:Todate, the investigation into the molecular mechanisms of testis development and spermatogenesis has largely focused on the role of protein-coding genes and small noncoding RNA including microRNAs and piRNAs. In recent years, long noncoding RNAs (lncRNAs) have been shown to play critical regulatory roles in mammalian development. To understand the role of lncRNAs in development of the mammalian testis and spermatogenesis, we firstly utilized commercial microarray to systematically investigate lncRNAs expression profiles of neonatal (6-day-old) and adult (8-week-old) mouse testis. By comparison the lncRNAs expression profiles of two developmental stage of testes, we obtained the differentially expressed lncRNAs and examined their genomic context, promoter characteristics, neighbored protein-coding genes, provide an important foundation for future research on molecular mechanisms of lncRNAs in mammalian testis development and spermatogenesis.

2015-01-01 | GSE43442 | GEO

A comprehensive roadmap of spermatogenesis and testis niche from single-cell RNA-seq

Project description:Continuous sperm production is not necessary for the survival of the organism, but is essential to maintain a species. The process of spermatogenesis is comprised of three phases: mitotic proliferation, meiosis, and spermiogenesis. To illuminate germline intrinsic and extrinsic programs, we performed single-cell RNA sequencing on ~35K cells from the adult mouse testis. This analysis provides a comprehensive molecular atlas of the testis, identifying both known and novel cell types. We demonstrate for the first time the continuous nature of germ cell differentiation, provide molecular signatures and subtype-specific molecular markers, and identify several novel candidate regulators of spermatogenesis. Finally, we demonstrate in vivo using spatial mapping that germ and somatic cell molecular subtypes correspond to previously defined histological cell types residing at different stages of seminiferous epithelial cycle. Taken together, our results unveil the complexity of the testis, and provide a global, unbiased roadmap of the in vivo gametogenesis program.

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