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Expression data from whole blood samples of Rwandan adults with mild malaria with matched sample thirty days later (convalescence)

ABSTRACT: Host factors governing mild disease in adults who have developed clinical immunity to Plasmodium falciparum may provide insights for disease altering vaccine or interventions, to prevent severe malaria We used microarrays of whole blood samples during mild malaria and compared them in a paired analysis to whole blood microarray 30 days later. (n=19 pairs)

ORGANISM(S): Homo sapiens

PROVIDER: GSE64338 | GEO | 2014/12/19

SECONDARY ACCESSION(S): PRJNA270766

REPOSITORIES: GEO

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Expression data from whole blood samples of Rwandan adults with mild malaria with matched sample thirty days later (convalescence)

Project description:Host factors governing mild disease in adults who have developed clinical immunity to Plasmodium falciparum may provide insights for disease altering vaccine or interventions, to prevent severe malaria We used microarrays of whole blood samples during mild malaria and compared them in a paired analysis to whole blood microarray 30 days later. (n=19 pairs) 3mL of whole blood was immediately placed in Tri-Reagent BD, frozen; high quality pairs were selected, were randomly selected for hybridization and analysis. Sense strand cDNA was generated from total RNA using the Ambion® WT Expression Kit (Life Technologies, Grand Island, NY) and labeling was done with the GeneChip® WT Terminal Labeling Kit (Affymetrix, Santa Clara, CA) for use with the GeneChip® Human Gene 1.0 ST Arrays (Affymetrix). The array image was generated by a high-resolution GeneArray Scanner 3000 7G (Affymetrix).

2014-12-19 | E-GEOD-64338 | biostudies-arrayexpress

Paired whole blood human transcription profiles from children with severe malaria and mild malaria

Project description:Whole blood transcriptomes from a longitudinal study of 5 Malawian children who first present with severe Plasmodium falciparum malaria, and return in one month with mild malaria We used microarrays to identify transcripts that were associated with each clinical presentation. A blood sample was taken upon presentation during the severe and mild malaria episodes in 5 Malawian children (total n=5 pairs) followed by RNA extraction and hybridization on Affymetrix GeneChip Human Gene 1.0 ST Array, using a paired analysis

2012-01-11 | E-GEOD-33811 | biostudies-arrayexpress

Paired whole blood human transcription profiles from children with severe malaria and mild malaria

2012-01-11 | GSE33811 | GEO

Blood transcriptional profiles discriminates cerebral and mild malaria patient living in Senegal

Project description:PBMCs were obtain from mild malaria and cerebral malaria patient at the onset of P. falciparum infection. Patient recruitment took place in two hospital centers in Senegal Total RNAs from PBMCs of mild and cerebral malaria patients were profiled after hybridization with Agilent SurePrint G3 Human GE 8x60K Microarray to identify blood biomarkers for cerebral malaria phenotype Plasmodium falciparum malaria remains a major health problem in Africa. The mechanisms of pathogenesis leading to the severe form of the disease are not fully understood. Blood transcriptional profiles were investigated in patient with cerebral malaria, noncerebral malaria , and mild malaria by using the microarray technology. We identified a set of 447 genes that was differentially expressed between the three patient groups after a false discovery rate of 10%. Since the cerebral patients displayed a particular transcriptional pattern, we focused our analysis on the differences between cerebral malaria patients and mild malaria patients. We further found 849 genes differentially expressed after a false discovery rate of 10%. We validated our results by using the qPCR method for 5 genes. The enrichment analysis of their functional annotation indicates that genes involved in A, B, C pathways play a role in the occurrence of cerebral malaria. These results provide new insight into the potential effect of the dysregulation of gene expression and specific pathways. Host genetic variation may partly explain such alteration of gene expression. Further studies are required to investigate this in African populations.

2019-10-07 | GSE116306 | GEO

Spatial transcriptomics of mouse spleens during malaria convalescence.

Project description:Naturally-acquired immunity to blood-stage malaria is associated with several effector CD4 + T subsets including germinal centre (GC) Tfh, Th1 and Tr1 cells. Here, we mapped the locations of effector CD4+ T cell subsets during post-Plasmodium convalescence using Slide-seqV2.

2024-05-02 | GSE234253 | GEO

Blood transcriptional profiles discriminates cerebral and mild malaria patient living in Senegal

Project description:Blood transcriptional profiles discriminates cerebral and mild malaria patient living in Senegal

| PRJNA478031 | ENA

Klebsiella phage vB_KpM-Mild

Project description:Klebsiella phage vB_KpM-Mild, whole genome

| PRJEB40177 | ENA

Homo sapiens

Project description:Paired whole blood human transcription profiles from children with severe malaria and mild malaria

| PRJNA148135 | ENA

Effect of mild hyperthermia on Mk differentiation

Project description:Physical factors can have major influences on the proliferation and differentiation fate of hematopoietic stem cells in culture. Recently, we demonstrated that cord blood CD34+ cells undergo accelerated and increased megakaryocyte differentiation when incubated at 39°C. In this study, we investigated in detail the impacts of mild hyperthermia on the kinetics of megakaryocyte differentiation, maturation, polyploidization and cell viability. The qualitative and quantitative effects on Mk differentiation were found to be rapidly induced, and optimization of the culture length at 39°C led to greater Mk yields. Mild hyperthermia did not promote endomitosis of cord blood-derived Mk, but rather led to a small reduction in the proportion of polyploid Mk. Moreover, it had little impact on viability but induced a significant shift in the proportion of cells undergoing apoptosis at 37°C to necrosis at 39°C. Finally, our results suggest that the effects on megakaryocyte differentiation could be the consequences of aberrant expression of key megakaryocyte transcription factors induced by mild hyperthermia. Keywords: Effect of mild hyperthermia on Mk differentiation

2007-12-01 | GSE9218 | GEO

Transcription profiling of blood in mice exposed Unpredicatble Chronic Mild Stress and treated with fluoxetine

Project description:Groups of 8 adult BALB/c male mice were either untreated or exposed for 9 weeks to unpredicatble chronic mild stress (UCMS), or exposed for 7 weeks to UCMS and treated for the last 5 weeks with fluoxetine (Flx) diluted in drinking water, or untreated for 2 weeks and received Flx for 5 weeks. Total RNAs from whole blood (WB) were profiled after hybridization with Agilent SurePrint G3 Mouse GE 8x60K Microarray v1 to identify transcriptional biomarkers of major depression.

2017-09-14 | GSE84184 | GEO

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