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GLI1-regulated genes in human mammary epithelial cells and human breast cancer cells

ABSTRACT: To clarify transcriptional target genes of GLI1 in human mammary epithelial cells and breast cancer cells, primary culture cells of human mammary epithelium HMEC and breast cancer cell line MCF-7 were lentivirally transduced by either GLI1 or its control LacZ. Their RNA samples were served for expression analysis using AGILENT human 8x60K cDNA microarray.

ORGANISM(S): Homo sapiens

PROVIDER: GSE64350 | GEO | 2015/04/28

SECONDARY ACCESSION(S): PRJNA270805

REPOSITORIES: GEO

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GLI1-regulated genes in human mammary epithelial cells and human breast cancer cells

Project description:To clarify transcriptional target genes of GLI1 in human mammary epithelial cells and breast cancer cells, primary culture cells of human mammary epithelium HMEC and breast cancer cell line MCF-7 were lentivirally transduced by either GLI1 or its control LacZ. Their RNA samples were served for expression analysis using AGILENT human 8x60K cDNA microarray. Array analysis of HMEC and MCF-7 expressing either GLI1 or LacZ. 1 color method was employed.

2015-04-28 | E-GEOD-64350 | biostudies-arrayexpress

Downstream genes regulated by TSHZ2 in MCF-7 cells

Project description:To clarify downstream genes regulated by TSHZ2, MCF-7 cells were lentivirally transduced by TSHZ2 and its control LacZ and served their RNA samples for gene expression analysis using AGILENT human 8x60K cDNA microarray.

2016-01-11 | GSE64351 | GEO

Downstream genes regulated by TSHZ2 in MCF-7 cells

2016-01-11 | E-GEOD-64351 | biostudies-arrayexpress

Gene expression - GLI1 induced mammary gland tumours

Project description:Increased expression of GLI1 is associated with poor prognosis for some breast cancer subtypes. A conditional transgenic GLI1 expressing mouse model, with or without heterozygous deletion of Trp53, was used to generate and study GLI1 induced mammary gland tumours. Tumour tissue was serially orthotopically transplanted for at least 10 generations in NSG mice.

2019-06-30 | E-MTAB-7531 | biostudies-arrayexpress

Human Breast Cancer Cell MCF-7: Control vs. Gli1 Transfected

Project description:Transcriptional profiling of breast cancer cell line MCF-7 stably transfected with pcDNA3.1-Gli1 comparing control ones transfected with CON

2014-11-30 | GSE53729 | GEO

GLI1 ChIP-Seq in non-irradiated versus irradiated SUM1315 cells

Project description:Changes in the GLI1 cistrome following ionizing radiation were investigated using ChIP-Seq in SUM1315 triple-negative breast cancer cells.

2020-10-22 | GSE146237 | GEO

Chromatin accessibility in response to knockdown of GLI1 and SMARCA2 in GLI1-mediated gene expression.

Project description:GLI1 is a transcription factor correlated to decreased survival in several cancers. We have identified SMARCA2 as a co-regulator that enhances GLI1-mediated transcriptional activity and functions through the C-terminal transcriptional activation domain of GLI1. Central domains including the ATPase motif of SMARCA2 physically interact with GLI1. Evaluation of DNA density indicates GLI1, like SMARCA2, can increase the DNA accessibility with a preference for sites distal to gene transcription start sites and outside the promoter regions (i.e. enhancers). The putative enhancers where accessibility is decreased by the knock down of GLI1 and SMARCA2 are located cis to genes, such as HHIP, that are regulated by GLI1 and implicated in cancer functions. At the putative enhancer for HHIP, the localization of SMARCA2 is at least partially dependent on GLI1’s presence. Understanding this transcriptional regulation by GLI1 and SMARCA2 through altering chromatin accessibility at enhances can provide additional therapeutic targets for cancers dependent on GLI1.

2020-05-11 | GSE143684 | GEO

GLI1 knock-down in Panc1 cells

Project description:Panc1 (human pancreatic adenocarcinoma cells) cells were transfected with control siRNA (targeting firefly luciferase, siLuc) or siRNA targeting GLI1 (siGLI1, Pool of four siRNAs). 72h following transfection, RNA was prepared for array analysis.

2012-03-31 | E-MEXP-3379 | biostudies-arrayexpress

Comprehensive Functional Annotation of Seventy-One Breast Cancer Risk Loci

Project description:We report the application of FAIRE seq in Human Mammary Epithelial Cells for identifying the breast cancer risk functional SNPs in enhancer region. Examination of FAIRE assay in HMEC

2013-05-10 | E-GEOD-46074 | biostudies-arrayexpress

Expression data from C2C12 myotubes infected with adenovirus expressing Gli1

Project description:We sought to determine the effects of over-expression of Gli1 on gene expression in C2C12 myotube cultures. C2C12 myoblasts were induced to differentiate for 4 days. At that time, when >80% of nuclei were incorporated into multi-nucleated syncitial myotubes, we infected the cultures with recombinant adenovirus expressing GFP alone or GFP and a full length human Gli1. Media was changed 12 hours later. Cultures were lysed 60 hours after the initial infection. Gli1 over-expression induces de-differentiation of myotubes and proliferation of myoblasts. Results provide insight into the molecular basis of SHH signaling on skeletal muscle cells.

2013-12-01 | E-GEOD-37702 | biostudies-arrayexpress

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