Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparison of gene expression between wild-type and Osterix1-Cre:Hdac3 CKO BMSC-derived osteoblasts

ABSTRACT: Bone marrow stromal cells (BMSC) were isolated from femurs and tibias of littermate wildtype and CKO mice. Cells were plated in osteogenic culture medium (alphaMEM, 20% FBS, 1% antibiotic/antimycotic, 1% non-essential amino acids, 50 ug/mL ascorbic acid, 10 mM beta glycerophosphate, 10^-7 M dexamethasone) and cultured for 7 days prior to RNA isolation.

ORGANISM(S): Mus musculus

PROVIDER: GSE64432 | GEO | 2016/01/31

SECONDARY ACCESSION(S): PRJNA271004

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Effects of prostate cancer cells on osteoblasts

Project description:Primary murine osteoblasts were isolated form the calvariae of newborn mice. 10 days after the addition of ascorbic acid (50 µg/ml) and ß-glycerophosphate (10 mM), cells were serum-starved over night and then incubated for 6 hours with condtioned medium of MDA-PCa2b cells or conditioned medium of PC-3 cells Keywords: expression profiling by array

2010-07-01 | GSE17348 | GEO

Effect of LLC1 tumor cell conditioned medium on RNA expression in mouse osteoblasts (MC3T3 cells)

Project description:MC3T3 osteoblasts were grown in culture medium in the presence of 10 mM beta-glycerophosphate and and 100 micro g per mL of ascorbic acid and 25% LLC1 conditioned medium or vehicle for 7 days. Cells were lysed an RNA was isolated.

2023-04-15 | GSE152513 | GEO

Effects of prostate cancer cells on osteoblasts

Project description:Primary murine osteoblasts were isolated form the calvariae of newborn mice. 10 days after the addition of ascorbic acid (50 Âµg/ml) and Ã-glycerophosphate (10 mM), cells were serum-starved over night and then incubated for 6 hours with condtioned medium of MDA-PCa2b cells or conditioned medium of PC-3 cells Keywords: expression profiling by array The aim of the experiment was to identify transcriptional changes in osteoblasts caused by factors derived from two different prostate cancer cell lines

2010-07-30 | E-GEOD-17348 | biostudies-arrayexpress

A Global Transcriptome Analysis of BMSC Senescence

Project description:Bone marrow stromal cells (BMSCs) can be expanded by serial passage, but expansion is limited by cell senescence. The nature of changes associated with BMSC serial passages was assessed. Transcriptome analysis of 10 early and 15 late passage samples from 5 subjects revealed 2193 differentially expressed genes; those highly expressed in early passage cells were overrepresented in skeletal system development, embryonic morphogenesis, tube morphogenesis, etc, while those highly expressed in the late passage BMSCs were overrepresented in nucleosome assembly; chromatin assembly, DNA packaging, etc. 57 BMSC samples from 7 donors were further analyzed for the transition from an early to late passage; 155 genes were highly correlated with BMSC senescence and a set of 24 genes was predictive of BMSCs lifespan. The change from an early to a late passage molecular signature occurred between passage 3 and 5. In contrast, senescence associated beta-galactosidase staining began to increase after passage 6 or 7 and colony formation efficiency began to fall after passage 7. These data indicated that the onset of molecular changes associated with BMSC passage varied among individuals and preceded changes in commonly used indicators of BMSC senescence. The set of 24 BMSC lifespan predictive genes will be useful in assessing the quality of clinical BMSC products.

2013-11-21 | GSE34303 | GEO

Quantitative analysis of miRNA changes in bone marrow stem cells under neural differentiation

Project description:Normal SJL mice, 6 to 8 weeks old, were used for the isolation of bone marrow stem cells (BMSC). Bone marrow cells were obtained from the femurs and tibias of euthanized mice by flushing with PBS. Cells were subjected to negative magnetic sorting using the Lineage Cell Depletion Kit. Isolation of murine bone marrow Lin-Sca-1+ cells was performed using MACS Sca-1 MultiSort Kit (fBMSC). The differentiation of neural stem cells was induced by removing the bFGF-containing medium and resuspending cells in fresh bFGF-free medium. Microarray analysis of miRNA expression profiles was performed comparing non differentiated bone marrow stem cells (fBMSC) to bone marrow stem cells differentiated for 4 or 7 days. The data are from adult mouse stem cells isolated from bone marrow

2013-01-19 | E-GEOD-43621 | biostudies-arrayexpress

Quantitative analysis of miRNA changes in bone marrow stem cells under neural differentiation

2013-01-19 | GSE43621 | GEO

CITE-seq of murine bone and marrow cells

Project description:To investigate heterogeneity of CD24 osteolineage cells at the single-cell level, we performed CITE-seq analysis with the CD24 antibody on bone and marrow mesenchymal cells from mouse femurs and tibias

2023-07-13 | GSE237301 | GEO

Expression data from primary osteoblasts isolated from calvaria of Gnptab(c.3082insC) knock-in mice compared with osteoblasts isolated from wild-type littermates

Project description:Mucolipidosis type II (MLII) is a severe inherited multisystemic disorder caused by mutations in the GNPTAB gene. Skeletal abnormalities are a predominant feature of MLII. Here we investigate the gene expression in a knock-in mouse model for mucolipidosis type II, generated by the insertion of a cytosine in the murine Gnptab gene (c.3082insC) that is homologous to a homozygous mutation in an MLII patient. Since osteoblasts are critically involved in regulating bone development and remodeling, a genome-wide expression analysis was performed with RNA isolated from primary cultures of osteoblasts originating from MLII knock-in mice (KI) compared to RNA from wild-type (WT) osteoblasts to identify dysregulated genes involved in pathogenic mechanisms. Primary osteoblasts were isolated from calvaria of 5-day-old wild-type (WT) and MLII knock-in littermates (KI). RNA was extracted at day 10 of differentiation induced by ascorbic acid and beta-glycerophosphate and hybridization on Affymetrix microarrays. We used preparations of RNA from two individual primary cultures of osteoblasts for every genotype (WT_OB_I, WT_OB_II, KI_OB_I, KI_OB_II) and compared WT vs KI samples.

2014-01-29 | E-GEOD-43854 | biostudies-arrayexpress

Analysis of human neurotrophins and receptors in bone marrow stromal cells (BMSC) treated with NGF or BDNF

Project description:BMSC gene expression profiling was completed to examine the baseline neurotrophin and receptor gene expression in BMSC and to evaulate changes in gene expression in response to NGF or BDNF stimulation In the study, primary BMSC were treated with recombinant human NGF or BDNF for 24 hours. Following treatment, RNA isolated from the BMSC were analyzed using the Human Neurotrophin and Receptor Gene Array HS-018

2010-05-01 | E-GEOD-18537 | biostudies-arrayexpress

Comparison of gene expression profile of gilthead seabream mineralization-induced VSa13 cells against controls

Project description:In the present study, the Agilent-016251 Sparus aurata oligo microarray platform (GPL6467) was used to compare expression profiles of mineralization-induced VSa13 cell cultures against untreated ones. ECM mineralization was induced for 4 weeks by supplementing medium with 50 µg/ml of L-ascorbic acid, 10 mM β glycerophosphate and 4 mM CaCl2. For each group, total RNA was extracted from three (3) independent biological replicates, each consisting of pools of cells. Data analysis demonstrated that expression profiles were strongly affected by ECM mineralization with hundreds of genes differentially expressed with relevant fold-change.

2011-06-01 | GSE18915 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data