Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Diet and phenobarbital affect cholesterol homeostasis and drug metabolism

ABSTRACT: High cholesterol diet and xenobiotic treatment induce changes in cholesterol homeostasis and drug metabolism. Mice were either 7 days on high cholesterol diet or were treated with phenobarbital. Liver samples were analyzed using Sterolgene v0 cDNA microarrays. Sterolgene microarray is a tool designed to enable focused studies of cholesterol homeostasis and drug metabolism. We show that one week of cholesterol diet down-regulates cholesterol biosynthesis and up-regulates xenobiotic metabolism (Cyp3 family). Phenobarbital treatment also up-regulates xenobiotic metabolism (Cyp2b and Cyp3a families). We can conclude that the Sterolgene series of cDNA microarrays represent novel original tool, enabling focused and cost-wise studies of cholesterol homeostasis and drug metabolism. Keywords: Treatment and diet effects

ORGANISM(S): Mus musculus

PROVIDER: GSE6447 | GEO | 2007/11/27

SECONDARY ACCESSION(S): PRJNA98643

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Diet and phenobarbital affect cholesterol homeostasis and drug metabolism

Project description:High cholesterol diet and xenobiotic treatment induce changes in cholesterol homeostasis and drug metabolism. Mice were either 7 days on high cholesterol diet or were treated with phenobarbital. Liver samples were analyzed using Sterolgene v0 cDNA microarrays. Sterolgene microarray is a tool designed to enable focused studies of cholesterol homeostasis and drug metabolism. We show that one week of cholesterol diet down-regulates cholesterol biosynthesis and up-regulates xenobiotic metabolism (Cyp3 family). Phenobarbital treatment also up-regulates xenobiotic metabolism (Cyp2b and Cyp3a families). We can conclude that the Sterolgene series of cDNA microarrays represent novel original tool, enabling focused and cost-wise studies of cholesterol homeostasis and drug metabolism. Keywords: Treatment and diet effects One group of mice was treated i.p. with 50 mg/kg of phenobarbital in vehicle (5% DMSO in corn oil). Untreated group was injected vehicle. Third group was 7 days on 1 % (w/w) cholesterol diet prior vehicle treatment. After 10 h animals were sacrificed and livers were stored. Pools of total RNA from two animals were mixed. Three pools of untreated and phenobarbital treated groups, and two pools of cholesterol diet group were co-hybridized with liver reference on Sterolgene v0 cDNA microarray. No dye-swaps were performed.

2010-05-25 | E-GEOD-6447 | biostudies-arrayexpress

High cholesterol diet and phenobarbital affect cholesterol homeostasis and drug metabolism

2007-11-27 | GSE6721 | GEO

Transcription profiling of mouse liver treated with phenobarbital and fed a high cholesterol diet an

2008-06-15 | E-GEOD-6721 | biostudies-arrayexpress

Mus musculus

Project description:Diet and phenobarbital affect cholesterol homeostasis and drug metabolism

| PRJNA98643 | ENA

Mus musculus

Project description:High cholesterol diet and phenobarbital affect cholesterol homeostasis and drug metabolism

| PRJNA98991 | ENA

Phenobarbital and TCPOBOP effects on CAR and PXR regulated genes involved in drug metabolism and cholesterol homeostasis

Project description:This SuperSeries is composed of the following subset Series: GSE12489: Effect of phenobarbital on CAR and PXR regulated genes involved in drug metabolism and cholesterol homeostasis GSE12509: Effect of TCPOBOP on CAR and PXR regulated genes involved in drug metabolism and cholesterol homeostasis Refer to individual Series

2008-08-23 | E-GEOD-12529 | biostudies-arrayexpress

TNFalpha interferes with adaptation to starvation and activates pro-atherogenic processes

Project description:Pathenogenesis of atherosclerosis results from the interactions between disrupted lipid homeostasis and immune response, but the molecular bridges between the major players are still a matter of controversy. We performed a systemic study of the imflammatory cytokine tumor necrosis factor alpha (TNF alpha), a well established anorexia agent, in the livers of the mice exposed to 20h-cytokine/starvation. We show that treatment with TNF-alpha reverses adaptations to fasting. Moreover, it up-regulates cholesterol biosynthesis and down-regulates bile acids synthesis, which leads to disrupted cholesterol homeostasis and pro-atherogenic changes. Interestingly, we found that TNF alpha also interferes with gene regulation of the xenobiotic metabolism. Keywords: treatment and diet effects Mice have been either saline treated (nontreated group), saline treated and fasted for 20h (fasted group), and TNF-alpha treated (30 mikrograms per animal) and fasted for 20h (TNF-alpha group). 3 biological replicas from fasted and TNF-alpha groups were co-hybridized with pool of nontreated group. No dye-swaps were performed.

2010-05-25 | E-GEOD-6423 | biostudies-arrayexpress

TNFalpha interferes with adaptation to starvation and activates pro-atherogenic processes

2007-11-27 | GSE6423 | GEO

Mus musculus

Project description:Effect of phenobarbital on CAR and PXR regulated genes involved in drug metabolism and cholesterol homeostasis

| PRJNA114219 | ENA

Project description:Phenobarbital and TCPOBOP effects on CAR and PXR regulated genes involved in drug metabolism and cholesterol homeostasis

| PRJNA112937 | ENA