Rapid, high efficiency isolation of pancreatic ß-cells

ABSTRACT: The ability to isolate pure pancreatic ß-cells would greatly aid multiple areas of diabetes research. We developed an exendin-4-like neopeptide conjugate for the rapid purification and isolation of functional pancreatic ß-cells. By targeting the glucagon-like peptide-1 receptor, ß-cells were isolated within an hour and were >99% pure. These studies were confirmed by immunostaining, confocal microscopy and microarray analysis on isolated cells. Gene expression profiling studies of the cytofluorometrically sorted ß-cells provided new insights into the genetic programs at play of different ages and stages during type-1 diabetes development. The described isolation method should have broad applicability to the ß-cell field.

ORGANISM(S): Mus musculus

PROVIDER: GSE64508 | GEO | 2015/09/04

SECONDARY ACCESSION(S): PRJNA271154

REPOSITORIES: GEO