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MeDIP-on-Chip for the Ciliary derived stem/progenitor cells and their differentiated progeny.

ABSTRACT: Whole genome profiling of DNA methylation in Ciliary derived stem/progenitor cells and differentiated cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE64546 | GEO | 2016/01/02

SECONDARY ACCESSION(S): PRJNA271286

REPOSITORIES: GEO

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MeDIP-on-Chip for the Ciliary derived stem/progenitor cells and their differentiated progeny.

Project description:Whole genome profiling of DNA methylation in Ciliary derived stem/progenitor cells and differentiated cells 6 samples total, 3 with CPE derived neurospheres and 3 with CPE derived differentiated progeny.

2016-07-04 | E-GEOD-64546 | biostudies-arrayexpress

ChIP-on-Chip for the Ciliary derived stem/progenitor cells and their differentiated progeny.

2016-01-02 | GSE65268 | GEO

ChIP-on-Chip for the Ciliary derived stem/progenitor cells and their differentiated progeny.

Project description:Whole genome profiling of histone methylation (H3K4me3, H3K27me3 and RNA Pol II) in Ciliary derived stem/progenitor cells and differentiated cells. In the present study we focus on the cellular differentiation that involves loss of pluripotency and gain of lineage and cell type-specific characteristics and we show developments from adult ciliary derived stem/progenitor cells to differentiated retinal neurons/glial cells of human cadaveric eyes in vitro. Ciliary pigment epithelial cells isolated from human cadaveric eye balls were cultured in the presence of mitogens like Epidermal Growth Factor (EGF) and Fibroblast Growth Factors (FGF) to generate neurospheres (Stem/progenitor population). The generated neurospheres were plated on laminin and poly-D-lysine-coated cover slips in the presence of the Brain derived neurotrophic factor (BDNF), Retinoic acid (RA) and 10% FBS for the differentiation (Retinal neuron/glial cells). Global Methylation patterns of histone H3K4me3, RNA Pol II and H3K27me3 were analysed in the present study. This work would provide an outline of epigenetic modifications in ciliary derived stem/progenitor cells and the progeny that underwent differentiation into retinal neurons/glial cells and present that specific histone methylations are involved in gene expression reprogramming during the process of differentiation.

2016-07-04 | E-GEOD-65268 | biostudies-arrayexpress

Whole gene expression profiling: A study for analysing the gene expression of the retinal stem cells and the differentiated cells

Project description:Comparitive analysis of the retinal stem/progentior cells derived from the ciliary and iris pigment epithelial cells and the subsequent differentiated cells derived from the stem/progenitor cells. Gene expression profiling has shown great promise in analysing the reprogramming pattern of the cells under various culture conditions, in this context we analysed the various differential gene expression pattern of the neurospheres derived from the ciliary and iris pigment epithelial cells, and their differentiated cells. This provide an insite for the stem cell transplantation studies. We analysed 3 biological cultures derived from each catogory like the primary cells of both ciliary and iris pigment epithelial cells, the stem/progenitor cells (Neurospheres), neurosphere derived differentiated cells. No technical replicates were performed.

2010-07-31 | E-GEOD-23340 | biostudies-arrayexpress

Whole gene expression profiling: A study for analysing the gene expression of the retinal stem cells and the differentiated cells

2010-07-31 | GSE23340 | GEO

DNA methylation in progenitor cells: MeDIP study

Project description:We surveyed DNA methylation profiles of all human RefSeq promoters in relation to gene expression and differentiation in adipose tissue, bone marrow and muscle mesenchymal progenitors, as well as in bone marrow-derived hematopoietic progenitors. We unravel strongly overlapping DNA methylation profiles between adipose stem cells (ASCs), bone marrow mesenchymal stem cells (BMMSCs) and muscle progenitor cells (MPCs), while hematopoietic progenitor cells (HPCs) are more epigenetically distant from MSCs seen as a whole. Differentiation resolves a fraction of methylation patterns common to MSCs, generating epigenetic divergence. DNA was isolated from MSCs isolated from various tissues and from differentiated cells, immunoprecipitated using antibodies to 5-methylcytosine, and co-hybridized onto Nimblegen promoter arrays together with input DNA.

2010-05-12 | E-GEOD-19794 | biostudies-arrayexpress

MicroRNA profiling of rat CG-4 oligodendrocyte progenitor cells and differentiated cells

Project description:Comparison of microRNA expression profile of CG-4 oligodendrocyte progenitor cells and differentiated cells.

2009-02-01 | E-MEXP-1764 | biostudies-arrayexpress

Smad3 binding regions in differentiated human progenitor epidermal keratinocytes (HPEK).

Project description:Smad3 binding regions in differentiated human progenitor epidermal keratinocytes (HPEK).

| PRJNA747268 | ENA

DNA methylation in progenitor cells: MeDIP study

2010-05-12 | GSE19794 | GEO

A single-cell transcriptomic atlas of the human ciliary body

Project description:The ciliary body is required for the maintenance of intraocular pressure and immunity as well as vision accommodation. We report a comprehensive cell atlas of human ciliary body from single-cell RNA sequencing (scRNAseq)

2022-10-05 | GSE206026 | GEO

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