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Changes in transcript expression in circulating whole blood resulting from exposure to neurotoxic doses of D-amphetamine or Heat Stroke/Hyperthermia

ABSTRACT: These experiments were designed to detect transcript (mRNA) changes in whole circulating blood in animals exposed to D-amphetamine under neurotoxic and non-neurotoxic conditions, or subjected to elevated environmental temperatures that produced a hyperthermia very similar to heat stroke. The study objectives were: 1) to detect transcript changes in blood due to life-threatening hyperthermia produced by elevated environmental temperatures (39°C, produces no or minimal neurotoxicity); 2) detect transcripts that could serve as biomarkers specific for neurotoxic amphetamine exposures and not seen with environmentally-induced hyperthermia; and 3) determine the transcript changes related to the immune system in circulating blood produced by either non-neurotoxic or neurotoxic amphetamine exposures. Amphetamine effects on gene expression are dependent on body temperature and indicate that many significant changes in genes related to the immune system occur, some likely in response to damage, even when animals remain normothermic during amphetamine exposure. Also, hyperthermia alone produces many changes in immune related genes in blood

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE64778 | GEO | 2015/12/08

SECONDARY ACCESSION(S): PRJNA271851

REPOSITORIES: GEO

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Changes in transcript expression in circulating whole blood resulting from exposure to neurotoxic doses of D-amphetamine or Heat Stroke/Hyperthermia

Project description:These experiments were designed to detect transcript (mRNA) changes in whole circulating blood in animals exposed to D-amphetamine under neurotoxic and non-neurotoxic conditions, or subjected to elevated environmental temperatures that produced a hyperthermia very similar to heat stroke. The study objectives were: 1) to detect transcript changes in blood due to life-threatening hyperthermia produced by elevated environmental temperatures (39Â°C, produces no or minimal neurotoxicity); 2) detect transcripts that could serve as biomarkers specific for neurotoxic amphetamine exposures and not seen with environmentally-induced hyperthermia; and 3) determine the transcript changes related to the immune system in circulating blood produced by either non-neurotoxic or neurotoxic amphetamine exposures. Amphetamine effects on gene expression are dependent on body temperature and indicate that many significant changes in genes related to the immune system occur, some likely in response to damage, even when animals remain normothermic during amphetamine exposure. Also, hyperthermia alone produces many changes in immune related genes in blood Five groups of animals were necessary to meet the study objectives. All groups were given 4 injections of either normal saline or amphetamine, and the injections were sequentially given with 2 h between each injection. Dosing started at 7:30 to 8:30 a.m. The groups are: 1) normothermic controls given normal saline in a 22.5Â°C environment; 2) controls given normal saline in a 16Â°C environment (also remained normothermic); 3) environmentally-induced hyperthermia given saline in a 39Â°C environment; 4) non-neurotoxic amphetamine given in a 16Â°C environment and 5) neurotoxic amphetamine group given amphetamine in a 22.5Â°C environment. Note the the saline controls (normothermic data) is contained in a separate but linked GEO file GSE62368

2015-12-08 | E-GEOD-64778 | biostudies-arrayexpress

Transcript expression in circulating whole blood after saline injections

2015-12-08 | GSE62368 | GEO

Transcript expression in circulating whole blood after saline injections

Project description:These experiments were designed to produce the appropriate controls so that their cohort's transcript (mRNA) changes in whole circulating blood could be determined. The cohorts were exposed to D-amphetamine under a neurotoxic and non-neurotoxic condition or subjected to elevated environmental temperatures that produced a hyperthermia very similar to heat stroke. The cohort data is in a separate, but linked, GEO file. Animals were given four doses of 1 ml/ kg of normal saline with two hours in between each dose. The animals body temperature was determined every 1 h and the rats were sacrificed 3 h after the last saline injection. Animals were not given access to food or water during this period so that they were more suitable controls for the amphetamine treatment groups

2015-12-08 | E-GEOD-62368 | biostudies-arrayexpress

Comparison of the Global Gene Expression of Choroid Plexus and Meninges and Associated Vasculature under Control Conditions and after Pronounced Hyperthermia or Amphetamine Toxicity

Project description:These tissue were harvested to complement and extend the studies that generated GSE23093. They served 3 purposes; 1) identify genes important to choroid plexus function and compare them with those important for meninges and associated vasculature (MAV) function, 2) determine genes in the choroid plexus and sensitivity hyperthermia and amphetamine toxicity, 3) identify the important gene expression changes related to the immune system in MAV, choroid plexus and trunk' blood Gene mRNA expression patterns in choroid plexus and trunk blood were determined under control conditions as well as after (3 hr and 1day) exposure to either environmentally-induced hyperthermia or neurotoxic doses of amphetamine. This data was analyzed and compared to data from meninges and associated vasculature previously deposited in GEO. The data gathered under control conditions was used to further understand how the choroid plexus and meninges and associated vasculature might function to generate and regulate the cerebrospinal fluid. The expression patterns in the choroid plexus after environmentally-induced hyperthermia or neurotoxic doses of amphetamine was determine its damage and protective responses. The expression patterns after environmentally-induced hyperthermia or neurotoxic doses of amphetamine were compared among choroid plexus, meninges and associated vasculature and blood were analyzed to determine immune system responses.

2012-08-01 | E-GEOD-29733 | biostudies-arrayexpress

Changes in miRNA expression in circulating whole blood resutling from exposure to neurotoxic doses of D-amphetamine

Project description:These experiments were designed to compare the miRNA expression in whole circulating blood in animals exposed to D-amphetamine to the expression under control condition. This is the final set of RNA seq data to be generated by the experiments that have previously generated mRNA transcriptomes (GSE62368 and GSE64778). The study objectives were to detect miRNA changes in blood resulting from a neurotoxic exposure to amphetamine that may result from organ and tissue damage or immune function changes Amphetamine effects on gene expression are dependent on body temperature and indicate that many significant changes in genes related to the immune system occur, some likely in response to damage, even when animals remain normothermic during amphetamine exposure. Also, hyperthermia alone produces many changes in immune related genes in blood

2018-12-18 | GSE115835 | GEO

Rattus norvegicus

Project description:Changes in transcript expression in circulating whole blood resulting from exposure to neurotoxic doses of D-amphetamine or Heat Stroke/Hyperthermia

| PRJNA271851 | ENA

Comparison of the global gene expression of choroid plexus and meninges and associated vasculature under control conditions and after pronounced hyperthermia or amphetamine toxicity

2012-08-02 | GSE29733 | GEO

Changes in miRNA expression in circulating whole blood resutling from exposure to neurotoxic doses of D-amphetamine

Project description:Changes in miRNA expression in circulating whole blood resutling from exposure to neurotoxic doses of D-amphetamine

| PRJNA476238 | ENA

Transcriptional impact of organophosphate and metal mixtures on olfaction: copper dominates the chlorpyrifos-induced response in adult zebrafish.

Project description:Chemical exposures in fish have been linked to loss of olfaction leading to an inability to detect predators and prey and decreased survival. However, the mechanisms underlying olfactory neurotoxicity are not well characterized, especially in environmental exposures which involve chemical mixtures. We used zebrafish to characterize olfactory transcriptional responses by two model olfactory inhibitors, the pesticide chlorpyrifos (CPF) and mixtures of CPF with the neurotoxic metal copper (Cu).

2013-05-21 | GSE47039 | GEO

Transcriptional impact of organophosphate and metal mixtures on olfaction: copper dominates the chlorpyrifos-induced response in adult zebrafish.

2013-05-21 | E-GEOD-47039 | biostudies-arrayexpress

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