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RNA sequencing of ATCC 17978hm Acineotbacter baumannii strain and CpdA mutant

ABSTRACT: Difference in RNA expression levels between Acinetobacter baumannii cells expressing high and low levels of cyclic AMP

ORGANISM(S): Acinetobacter baumannii

PROVIDER: GSE64935 | GEO | 2015/06/21

SECONDARY ACCESSION(S): PRJNA272532

REPOSITORIES: GEO

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RNA sequencing of ATCC 17978hm Acineotbacter baumannii strain and CpdA mutant

Project description:Difference in RNA expression levels between Acinetobacter baumannii cells expressing high and low levels of cyclic AMP Total RNA obtained from Acineotbacter baumannii bacterial cells in Log phase gown in MH broth culture, isolated RNA in triplicate from three expreiment. cpdA::Tn mutant and 17978hm strain compared. Assessing increased levels of cAMP within the cell

2015-06-21 | E-GEOD-64935 | biostudies-arrayexpress

Multi-omics approach to study global changes in a triclosan mutant strain of Acinetobacter baumannii ATCC 17978.

Project description:Acinetobacter baumannii AB042, a triclosan-resistant mutant, was examined for modulated gene expression using whole genome sequencing, transcriptomics, and proteomics in order to understand the mechanism of triclosan-resistance as well as its impact on A. Baumannii.

2016-09-28 | GSE87398 | GEO

RNA-seq of Acinetobacter baumannii ATCC 17978 Lab-WT: under blue light

Project description:Comparison for gene expression of Acinetobacter baumannii Lab-WT under blue light irradiation

2022-01-31 | GSE195643 | GEO

RNA-seq of Acinetobacter baumannii ATCC 17978: deleted effects of dna adenine methylase

Project description:Comparison for gene expression of Acinetobacter baumannii ATCC 17978 by deleted effects of dna adenine methylase

2022-01-31 | GSE195621 | GEO

Transcriptional changes in response to OXA-23 overexpression in Acinetobacter baumannii ATCC 17978

Project description:Purpose: The goal of this study was to elucidate the collateral effects associated with OXA-23 overexpression on the Acinetobacter baumannii global transcriptome. Results: Besides the 99.73-fold increase in blaOXA-23 transcript upon IPTG induction, no other transcripts showed more than a 2-fold change compared to the wildtype control. This suggests that OXA-23 over expression to levels similarly observed in multi drug resistant A. baumannii clinical isolates does not effect the transcriptome.

2021-11-15 | GSE185203 | GEO

Transcription profiling of Acinetobacter baumannii multi-drug resistant strain and a susceptible reference strain - Abefflux

Project description:Transcriptomic comparison of a multidrug resistant Acinetobacter baumannii strain and a susceptible reference strain.

2009-11-20 | E-MEXP-2254 | biostudies-arrayexpress

RNA-Seq of Acinetobacter baumannii ATCC 17978 and a gene knockout strain of putative response regulator gene A1S_2006 from the ATCC 17978 background

Project description:The putative response regulator gene A1S_2006 of Acinetobacter baumannii ATCC 17978 was knocked out using a homologous recombination method to assess the effects of that gene knockout in a global transcriptome landscape. This was done with an objective to better understand the cellular processes that are regulated by the response regulator protein encoded by A1S_2006 gene.

2019-04-04 | E-MTAB-7815 | biostudies-arrayexpress

Comparative transcriptional analysis of Acinetobacter baumannii strain ATCC 17978 and strain 17978hm

Project description:In this study the transcriptomes of Acinetobacter baumannii strains ATCC 17978 and 17978hm were compared. Strain 17978hm is a hns knockout derivative of strain ATCC 17978. Strain 17978hm displays a hyper-motile phenotype on semi-solid Mueller-Hinton (MH) media (0.25% agar). ATCC 17978 and 17978hm from an 37C overnight culture were transferred to the centre of the semi-solid MH plate and incubated at 37C for 8 hours. Only 17978hm cells displayed a motile phenotype and covered the complete surface of the plate. These motile 17978hm cells and the non-motile wild-type ATCC 17978 cells were harvested and RNA was isolated. The comparative transcriptome analysis was performed using the FairPlay labeling kit and a custom made Agilent MicroArray with probes designed to coding regions of the ATCC 17978 genome. The data was analyzed using Agilent GeneSpring GX9 and the significance analysis of microarray MS Excel add-on. The motile 17978hm cells and the non-motile wild-type ATCC 17978 cells were harvested and RNA was isolated. The comparative transcriptome analysis was performedThe probes on the microarray cover all predicted open reading frames (at least 4 per ORF) and additional replicates of housekeeping genes of the A. baumannii ATCC 17978 genome .

2013-08-05 | E-GEOD-40681 | biostudies-arrayexpress

Comparative transcriptional analysis of Acinetobacter baumannii strain ATCC 17978 and strain 17978hm

2013-08-05 | GSE40681 | GEO

The primary transcriptome of Acinetobacter baumannii ATCC 17978

Project description:Transcriptomics by RNA-seq provides unparalleled insight into bacterial gene expression networks, enabling a deeper understanding of the regulation of pathogenicity, mechanisms of antimicrobial resistance, metabolism, and other cellular processes. Here we present the transcriptome architecture of Acinetobacter baumannii ATCC 17978, a species emerging as a leading cause of antimicrobial resistant nosocomial infections. Differential RNA-seq (dRNA-seq) examination of model strain ATCC 17978 in 16 laboratory conditions identified 3731 transcriptional start sites (TSS), and 110 small RNAs, including the first identification of 22 sRNA encoded at the 3′ end of mRNA.

2018-06-25 | GSE103244 | GEO

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