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Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach

ABSTRACT: The analysis compares primary fibroblasts initially used for reprogramming, established marmoset ES cells and a marmoset iPS cell line which was generated witha non-viral approach using a six-factor-in-one-vector approach

ORGANISM(S): Callithrix jacchus

PROVIDER: GSE64966 | GEO | 2015/04/08

SECONDARY ACCESSION(S): PRJNA272606

REPOSITORIES: GEO

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Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach

Project description:The analysis compares primary fibroblasts initially used for reprogramming, established marmoset ES cells and a marmoset iPS cell line which was generated witha non-viral approach using a six-factor-in-one-vector approach Cells were grown under standard conditions for marmoset pluripotent stem cells and primary fibroblasts without additional treatment. For each cell type at least four independent cell preparations were used.

2015-04-08 | E-GEOD-64966 | biostudies-arrayexpress

Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach.

Project description: Not available

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Establishing a common marmoset induced pluripotent stem cell line NM-iPS by an all-in-one episomal vector approach

Project description:Epstein-Barr virus (EBV)-based episomal vector system enables persistent transgene expression, which is advantageous for efficient derivation of transgene-free induced pluripotent stem cells (iPSCs) without viral transduction. Here, we report establishment of an iPSC line from somatic fibroblasts of a neonatal common marmoset monkey (marmoset; Callithrix jacchus) using an all-in-one episomal vector that we newly developed. The established iPSC line, named NM-iPS, showed standard characteristics of pluripotency such as pluripotency-related marker expression, three germ layer differentiation, and normal karyotype (2n = 46). The novel iPSC line would be a useful resource for stem cell research using non-human primates.

2021-01-10 | GSE164497 | GEO

Establishing a common marmoset induced pluripotent stem cell line NM-iPS by an all-in-one episomal vector approach

Project description:Establishing a common marmoset induced pluripotent stem cell line NM-iPS by an all-in-one episomal vector approach

| PRJNA690949 | ENA

Transcriptomes analysis of marmoset liver progenitor cell line compared with adult marmoset liver

Project description:We report the liver progenitor cell related gene expression of established marmoset liver progenitor cell line and provide the whole gene expression transcriptomes of marmoset liver.

2018-01-01 | GSE99168 | GEO

Maternal weight affects placental DNA methylation of genes invovled in metabolic pathways in the common marmoset monkey (Callithrix jacchus)

Project description:Accumulating evidence suggests that dysregulation of placental DNA methylation (DNAm) is a mechanism linking maternal weight during pregnancy to metabolic programming outcomes. The common marmoset, Callithrix jaccus, is a platyrrhine primate species that has provided much insight into studies of the primate placenta, maternal condition, and metabolic programming, yet the relationships between maternal weight and placental DNAm are unknown. Here, we report genome-wide DNAm from term marmoset placentas using reduced representation bisulfite sequencing. We identified 74 genes whose DNAm pattern is associated with maternal weight during gestation. These genes are predominantly involved in energy metabolism and homeostasis, including regulation of glycolytic and lipid metabolic processes pathways.

2020-01-07 | GSE143200 | GEO

The transcriptomes of novel marmoset monkey embryonic stem cell lines reflect genomic features

Project description:We report the generation and characterization of four novel marmoset monkey ES cell lines, derived from natural preimplantation embryos. Two cell lines were obtained from non-compacted morulae (DPZcjESC1 and 2), while the other two cell lines were derived from a compacted morula (DPZcjESC3) and an expanded blastocyst (DPZcjESC4), respectively. DPZcjESC1 and 2 showed trisomies for one and two chromosomes respectively, while DPZcjESC3 and 4 are euploid. We detected no obvious differences between morula- and blastocyst-derived cell lines. However, quantitative chromosome-wise transcriptome analyses precisely reflected the trisomies. We also show that the female ES cell lines exhibit different states of X-inactivation which is also impressively reflected by the abundance of the lncRNA X inactive-specific transcript (XIST).

2016-06-01 | GSE70897 | GEO

The transcriptomes of novel marmoset monkey embryonic stem cell lines reflect genomic features

2016-06-01 | E-GEOD-70897 | biostudies-arrayexpress

RNA sequencing of marmoset embryonic development

Project description:Transcriptional profiling of common marmoset embryo stages spanning E5.0 to E7.0 was performed by RNA-seq.

2015-11-05 | E-MTAB-2959 | biostudies-arrayexpress

Attempts for deriving extended pluripotent stem cells from common marmoset embryonic stem cells

Project description:Extended pluripotent stem cells (EPSCs) derived from mice and humans showed an enhanced potential for chimeric formation. By exploiting transcriptomic techniques, we assessed the differences in gene expression profile between extended EPSCs derived from mice and humans, and those newly derived from the common marmoset (marmoset; Callithrix jacchus). Although the marmoset EPSC-like cells displayed a unique colony morphology distinct from murine and human EPSCs, they displayed a pluripotent state akin to embryonic stem cells (ESCs), as confirmed by gene expression and immunocytochemical analyses of pluripotency markers and three-germ-layer differentiation assay. Importantly, the marmoset EPSC-like cells showed interspecies chimeric contribution to mouse embryos, such as E6.5 blastocysts in vitro and E8.5 epiblasts in vivo in mouse development. Also, we discovered that the perturbation of gene expression of the marmoset EPSC-like cells from the original ESCs resembled that of human EPSCs. Thus, we established the efficacy of the method for the derivation of marmoset EPSCs

2022-01-01 | GSE189633 | GEO

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