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From the morphologic to the transcriptomic characterization of a compromised 3D in vitro model mimicking atopic dermatitis

ABSTRACT: To characterize our compromised reconstructed epidermis at the transcriptomic level, we have employed whole genome microarray expression profiling as a discovery platform to compare genes modification with those known in AD skins. To this purpose, normal human keratinocytes (NHK) were used to generate RE, treated or not with an inflammatory cocktail (Poly I:C, TNF-α, IL-4 and IL-13). Inflammation and cellular differentiation pathways were analysed and then confirmed by Q-PCR experiments.

ORGANISM(S): Homo sapiens

PROVIDER: GSE65003 | GEO | 2018/01/10

SECONDARY ACCESSION(S): PRJNA272686

REPOSITORIES: GEO

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