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Transcriptome comparison of the Streptococcus pneumoniae D39∆tpxD treated with 1mM to 0mM H2O2

ABSTRACT: Transcriptome comparison of the Streptococcus pneumoniae D39∆tpxD grown in THY medium till OD620=0.25 and then treated with or without 1mM H2O2 for 40mins.

ORGANISM(S): Streptococcus pneumoniae D39

PROVIDER: GSE65156 | GEO | 2017/07/27

SECONDARY ACCESSION(S): PRJNA273298

REPOSITORIES: GEO

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Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type treated with 1mM to with 0mM H2O2

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in THY medium till OD620=0.25 and then treated with or without 1mM H2O2 for 40mins.

2017-07-27 | GSE65155 | GEO

Streptococcus pneumoniae D39 spxB- mutant vs. D39 wild-type

Project description:Comparison of the Streptococcus pneumoniae D39 spxB- mutant vs D39. For DNA microarray analysis, D39 wild-type and two independently obtained D39spxB- mutants were grown as four, two and two biological replicates, respectively, in Glc-M17 under semi-aerobic conditions and harvested at an OD595 of approximately 0.25 (mid-exponential). The RNA of both D39spxB- strains was compared to D39 and the combined data was analyzed. All other procedures regarding microarray analyses were done as described before. A gene was considered differentially expressed when the fold change was ≥ 2, ≤ 0.5, with a Bayes p ≤ 0.00001 and when at least 7 measurements were available.

2013-08-08 | GSE45971 | GEO

Streptococcus pneumoniae D39 spxB- mutant vs. D39 wild-type

Project description:Comparison of the Streptococcus pneumoniae D39 spxB- mutant vs D39. For DNA microarray analysis, D39 wild-type and two independently obtained D39spxB- mutants were grown as four, two and two biological replicates, respectively, in Glc-M17 under semi-aerobic conditions and harvested at an OD595 of approximately 0.25 (mid-exponential). The RNA of both D39spxB- strains was compared to D39 and the combined data was analyzed. All other procedures regarding microarray analyses were done as described before. A gene was considered differentially expressed when the fold change was M-bM-^IM-% 2, M-bM-^IM-$ 0.5, with a Bayes p M-bM-^IM-$ 0.00001 and when at least 7 measurements were available. One condition design comparision of two strains including a dye swap.

2013-08-08 | E-GEOD-45971 | biostudies-arrayexpress

Aerobic and Anaerobic Growth of Streptococcus pneumoniae D39

Project description:The catalase-negative, facultative anaerobe Streptococcus pneumoniae D39 is naturally resistant to hydrogen peroxide (H2O2) produced endogenously by pyruvate oxidase (SpxB). Here, we investigate the adaptive response to endogenously produced H2O2. We show that lactate oxidase, which converts lactate to pyruvate, positively impacts pyruvate flux through SpxB and that ∆lctO mutants produce significantly lower H2O2. In addition, both the SpxB and pyruvate dehydrogenase complex (PDHC) pathways contribute to acetyl-CoA production during aerobic growth, and the pyruvate format lyase (PFL) pathway is the major acetyl-CoA pathway during anaerobic growth. Microarray analysis of the D39 strain cultured under aerobic vs. strict anaerobic conditions show up-regulation of spxB, a rhodanese-like protein (spd0091), tpxD, sodA, piuB, piuD and an Fe-S protein biogenesis operon under H2O2-producing conditions. Proteome profiling of H2O2-induced sulfenylation reveals that sulfenylation levels correlate with cellular H2O2 production, with endogenous sulfenylation of ≈50 proteins. Deletion of tpxD increases cellular sulfenylation 5-fold and has an inhibitory effect on ATP generation. Two major targets of protein sulfenylation are glyceraldehyde-3-phosphate dehydrogenase (GapA) and SpxB itself, but also include pyruvate kinase, LctO, AdhE and acetate kinase (AckA). Sulfenylation of GapA is inhibitory, while the effect on SpxB activity is negligible, consistent with this cell-abundant protein functioning as a “sink” for endogenous H2O2. Strikingly, four enzymes of capsular polysaccharide biosynthesis are sulfenylated, as are enzymes associated nucleotide biosynthesis via ribulose-5-phosphate. We propose that LctO/SpxB-generated H2O2 functions as a signaling molecule to down-regulate capsule production and drive altered flux through sugar utilization pathways.

2016-12-08 | GSE19791 | GEO

Spneu D39 wildtype grown in GM17 versus GM17 + 0.25 mM ZnSO4

Project description:This study addresses the response of Streptococcus pneumoniae to zinc. The wild-type strain D39 was grown in GM17 and GM17 with 0.25 mM of ZnSO4. The transcriptome under these conditions was compared by using microarrays. Keywords: transcriptome analysis of D39 wild-type compared with its isogenic glnPA double mutant

2008-05-17 | GSE11438 | GEO

Proteome analysis of Beas-2B cells with S. pneumoniae D39

Project description:Proteome analysis of Beas-2B cells with S. pneumoniae D39 after 9 h and 16 h of infection

2023-07-04 | PXD039059 | Pride

Expression data of H2O2 responding genes at Arabidopsis root tip

Project description:To identify genes that regulate root development in a hydrogen peroxide devendent manner, we performed a time course microarray analysis of root treated with 1mM H2O2.

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Impact of naiR delation on the transcriptome of Streptococcus pneumoniae D39

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 ΔnaiR to D39 wild type grown in CDM

2017-02-24 | GSE94511 | GEO

Impact of copY delation on the transcriptome of Streptococcus pneumoniae D39

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 copY-stop mutant to D39 wild-type grown in CDM without added copper.

2011-07-10 | GSE30414 | GEO

Streptococcus pneumoniae D39

Project description:Streptococcus pneumoniae D39 spxB- mutant vs. D39 wild-type

| PRJNA214485 | ENA