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Global transcriptomic changes induced by manipulate MSH1 gene in tomato using next generation sequencing

ABSTRACT: apical meristem tissues from 4-week-old wild type, epiF3, MSH1 gene mutant including (mild-DR and dwarf-DR) plants were used in this experiment.

ORGANISM(S): Solanum lycopersicum

PROVIDER: GSE65242 | GEO | 2015/02/23

SECONDARY ACCESSION(S): PRJNA273478

REPOSITORIES: GEO

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Global transcriptomic changes induced by manipulate MSH1 gene in tomato using next generation sequencing

Project description:apical meristem tissues from 4-week-old wild type, epiF3, MSH1 gene mutant including (mild-DR and dwarf-DR) plants were used in this experiment. For each genotype, the transcriptome was sequenced with 3 biological replicates.

2015-02-23 | E-GEOD-65242 | biostudies-arrayexpress

High-throughput Illumina sequencing of bisulfite transformed genomic DNA from Arabidopsis thaliana wild-type, msh1 mutant and MSH1-epiF3 lines

Project description:Arabidopsis msh1 mutants display developmental reprogramming (dr) phenotypes, include reduction in growth, enhanced branching, and delayed maturation and flowering time. MSH1-epi lines were derived by crossing MSH-dr lines with Col-0 wild type, followed by selection for homozygous MSH1/MSH1 F2 plants and serial self-pollination. MSH1-epiF3 plants displayed phenotypic variation in plant growth, showing enhanced growth, larger rosette diameter, thicker floral stems and earlier flowering time. We carried bisulfite sequencing and uncover the methylome changes accompany the heritable MSH1-epi phenotypes that condition dramatic variation in plant growth. 3 samples examined: wild type, Msh1-epiF3, msh1 mutant

2014-01-01 | E-GEOD-36783 | biostudies-arrayexpress

High-throughput Illumina sequencing of bisulfite transformed genomic DNA from Arabidopsis thaliana wild-type, msh1 mutant and MSH1-epiF3 lines

2014-01-01 | GSE36783 | GEO

MSH1 disruption in plants triggers RdDM-mediated reprogramming of phenotypic plasticity (Bisulfite-Seq II)

Project description:To generate msh1 memory revertant population, msh1 memory plant was used to pollinate Col-0 to generate F1 population. Derived F1 progenies were self-pollinated to generate F2 populations. F2 plants were self-pollinated to produce F3 families that were segregationg for the msh1 memory phenotype (small dwarf plants with delayed flowering).

2022-07-08 | GSE192432 | GEO

Bisulfite methylome sequencing of msh1 memory line in tomato

Project description:MSH1 depletion caused whole genome wide Methylome changes in Arabidopsis, to conduct comparative genomic analysis, here we generate methylome for MSH1-RNAi line and transgene segregated out msh1 memory line in tomato

2018-06-30 | GSE105008 | GEO

Small RNA sequencing of progenies of Col-0 grafted on msh1; Col-0 grafted on dcl2,3,4,msh1; msh1 mutant; dcl2,3,4,msh1 quadruple mutant in Arabidopsis [msh1_graft_sRNA-seq]

Project description:Col-0 floral stem was grafted on the msh1 mutant (Col-0/msh1); on the dcl2,3,4,msh1 quadruple mutant (Col-0/dcl2,3,4,msh1). Seeds were collected from the grafted Col-0 scion after grafts were established. Seed coming from the graft then were grown on the peat mix, leaf tissue was collected at the bolting and used for the small RNA sequencing. Tissue from the msh1 mutant and dcl2,3,4,msh1 quadruple mutants used as rootstocks was similarly collected at the bolting stage and used for the small RNA sequencing.

2020-09-20 | GSE152567 | GEO

Bisulfite (methylome) sequencing of progenies of Col-0 grafted on msh1; Col-0 grafted on dcl2,3,4,msh1; Col-0 grafted on Col-0; msh1 mutant; dcl2,3,4,msh1 quadruple mutant in Arabidopsis [msh1_graft_BS-seq]

Project description:Col-0 floral stem was grafted on the msh1 mutant (Col-0/msh1); on the dcl2,3,4,msh1 quadruple mutant (Col-0/dcl2,3,4,msh1); on Col-0 (Col-0/Col-0). Seeds were collected from the grafted Col-0 scion after grafts were established. Seed coming from the graft then were grown on the peat mix, leaf tissue was collected at the bolting and used for the bisulfite sequencing (methylome). Tissue from the msh1 mutant and dcl2,3,4,msh1 quadruple mutants used as rootstocks was similarly collected at the bolting stage and used for the bisulfite sequencing.

2020-09-20 | GSE152564 | GEO

Transcript profiling of Arabidopsis msh1 mutants

Project description:The Arabidopsis msh1 mutant show various growth phenotype. We use microarray analysis to characterize gene expression pattern for two of the phenotypes - variegation and stunted growth. Wild type and msh1 RNAi variegated and stunted tissues were collected for RNA extration and hybridization on Affymetrix.

2012-07-11 | E-GEOD-35893 | biostudies-arrayexpress

RNA sequencing of progenies of Col-0 grafted on msh1; Col-0 grafted on dcl2,3,4,msh1 quadruple mutant in Arabidopsis [msh1_graft_root_RNA-seq]

Project description:Col-0 floral stem was grafted on the msh1 mutant (Col-0/msh1) and on the dcl2,3,4,msh1 quadruple mutant (Col-0/dcl2,3,4,msh1). Seeds were collected from the grafted Col-0 scion after grafts were established. Seed coming from the graft then were grown on 0.5M MS growth medium and root tissue was collected after 12 days and used for the RNA sequencing.

2020-09-20 | GSE152566 | GEO

Transcript profiling of Arabidopsis albino msh1 mutants

Project description:The soybean msh1 RNAi transgenic line show various growth phenotype. We use microarray analysis to characterize gene expression pattern for two of the phenotypes - variegation and stunted growth. Wild type and Arabidopsis msh1 mutant albino tissues were collected for RNA extration and hybridization on Affymetrix.

2011-11-29 | E-GEOD-30281 | biostudies-arrayexpress

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