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Comparison of Escherichia coli K-12 tynA- with wild type Escherichia coli K-12

ABSTRACT: Escherichia coli (E. coli) amine oxidase (ECAO) encoded by tynA gene has been one of the model enzymes to study the mechanism of oxidative deamination of amines to the corresponding aldehydes by amine oxidases. The biological roles of ECAO have been less addressed. Therefore we have constructed a gene deletion Escherichia coli K-12 strain, E. coli tynA-, and used the microarray technique to address its function by comparing the total RNA gene expression to the one of the wt. Our results suggest that tynA is a reserve gene for stringent environmental conditions and its gene product ECAO a growth advantage compared to other bacteria due to H2O2 production. Overall design: The effect of deleting the expression of E. Coli amine oxidase by deleting tynA gene to the expression of other genes was done by comparing the knock out strain to wild type strain in different time points.

INSTRUMENT(S): [E_coli_2] Affymetrix E. coli Genome 2.0 Array

SUBMITTER: Heli Elovaara  

PROVIDER: GSE65385 | GEO | 2015-09-30



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Primary Amine Oxidase of Escherichia coli Is a Metabolic Enzyme that Can Use a Human Leukocyte Molecule as a Substrate.

Elovaara Heli H   Huusko Teija T   Maksimow Mikael M   Elima Kati K   Yegutkin Gennady G GG   Skurnik Mikael M   Dobrindt Ulrich U   Siitonen Anja A   McPherson Michael J MJ   Salmi Marko M   Jalkanen Sirpa S  

PloS one 20151110 11

Escherichia coli amine oxidase (ECAO), encoded by the tynA gene, catalyzes the oxidative deamination of aromatic amines into aldehydes through a well-established mechanism, but its exact biological role is unknown. We investigated the role of ECAO by screening environmental and human isolates for tynA and characterizing a tynA-deletion strain using microarray analysis and biochemical studies. The presence of tynA did not correlate with pathogenicity. In tynA+ Escherichia coli strains, ECAO enabl  ...[more]

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