Transcriptomics,Genomics

Dataset Information

27

Knockdown of TSPAN8 gene expression in the SH-SY5Y neuroblastoma cell line


ABSTRACT: Bipolar disorder (BD) has an estimated heritability of about 80%. Different pathways and candidate genes may contribute to the pathogenesis of BD, but definite mechanisms are yet unresolved. In a previous study, we identified the single nucleotide polymorphism (SNP) rs4500567, located in the upstream region of Tetraspanin 8 (TSPAN8), to be associated with bipolar disorder (BD). The SNP´s minor allele results in a predicted binding site for the transcription factor Nuclear Factor Interleukin 3, regulated (NFIL3); luciferase assays confirmed allele-specific TSPAN8 expression levels. Overall design: To define the global effect of altered TSPAN8 gene expression, we performed a knockdown of TSPAN8 in SH-SY5Y cells followed by microarray analysis.

INSTRUMENT(S): [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

SUBMITTER: Claus Juergen Scholz 

PROVIDER: GSE65459 | GEO | 2018-06-26

REPOSITORIES: GEO

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Publications

The regulation of tetraspanin 8 gene expression-A potential new mechanism in the pathogenesis of bipolar disorder.

Schartner Christoph C   Scholz Claus-Jürgen CJ   Weber Heike H   Post Antonia A   Freudenberg Florian F   Grünewald Lena L   Reif Andreas A  

American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics 20170804 7


In a previous study, we identified the single nucleotide polymorphism (SNP) rs4500567, located in the upstream region of tetraspanin 8 (TSPAN8), to be associated with bipolar disorder (BD). Due to its proximal position, the SNP might have an impact on promoter activity, thus on TSPAN8 gene expression. We investigated the impact of rs4500567 on TSPAN8 expression in vitro with luciferase-based promoter assays in human embryonic kidney (HEK293) and neuroblastoma cells (SH-SY5Y), and its effect on e  ...[more]

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