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Genome wide analysis of Visceral adipose tissue CD14+ cells from obese subjects

ABSTRACT: Identification of the inflammatory signature in visceral adipose tissue CD14+ cells (adipose tissue macrophage) Total RNA obtained from CD14+ cells (Immunoselcted cells from stromal adipose tissue cells)

ORGANISM(S): Homo sapiens

PROVIDER: GSE65802 | GEO | 2015/02/11

SECONDARY ACCESSION(S): PRJNA275114

REPOSITORIES: GEO

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Genome wide analysis of Visceral adipose tissue CD14+ cells from Obese and obese diabetic subjects

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Genome wide analysis of Visceral adipose tissue CD14+ cells from obese subjects

Project description:Identification of the inflammatory signature in visceral adipose tissue CD14+ cells (adipose tissue macrophage) Total RNA obtained from CD14+ cells (Immunoselcted cells from stromal adipose tissue cells) Adipocytes and cells of the stroma vascular fraction (SVF) were obtained by collagenase digestion of adipose tissue. SVF cells were resuspended in endotoxin-free PBS supplemented with 2% FCS and 1 mM EDTA. Isolation of CD14+ using positive selection magnetic beads (Stemcell technologies) and CD4+ cells was performed using positive selection magnetic beads (Stemcell Technologies, Vancouver, Canada). An Illumina (San Diego, CA) RNA amplification kit (NuGEN, BiotinIL Module) was used according to the manufacturer's instructions to obtain biotinlabeled cDNA from 50 ng of total RNA extracted from adipose tissue CD14+ cells.

2015-02-11 | E-GEOD-65802 | biostudies-arrayexpress

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2014-01-24 | E-GEOD-54350 | biostudies-arrayexpress

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Project description:Visceral adipose tissue samples were obtained from severely obese individuals that underwent bariatric surgery. The goal of this study was to identify tissue specific methylation QTLs. Whole-transcriptome subcutaneous adipose tissue methylation levels were determined in 71 individuals with a BMI >35 kg/m2. Bisulphite converted DNA from the 71 visceral adipose tissue samples were hybridised to the Illumina Infinium 450k Human Methylation Beadchip.

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