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The effect of SubAB treatment on Wnt-High and Wnt-Low sorted human colon cancer stem cells

ABSTRACT: The effect of ER-stress and subsequent activation of the unfolded protein response was investigated in colon cancer stem cells and more differentiated colon cancer cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE65879 | GEO | 2015/02/12

SECONDARY ACCESSION(S): PRJNA275283

REPOSITORIES: GEO

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The effect of SubAB treatment on Wnt-High and Wnt-Low sorted human colon cancer stem cells

Project description:The effect of ER-stress and subsequent activation of the unfolded protein response was investigated in colon cancer stem cells and more differentiated colon cancer cells Human colon cancer derived spheroid cultures with a TCF/LEF driven GFP reporter for Wnt signaling activity were treated for 24 hours with SubAB or protease-dead SubABco (1Âµg/ml) Treated spheroid cultures were sorted into colon-cancer stem cell fraction (highest 10% Wnt-GFP) and differentiated colon cancer fraction (10% Lowest(Wnt Low) for micro array

2015-02-12 | E-GEOD-65879 | biostudies-arrayexpress

Expression data from TOP-GFP sorted colon cancer cells

2012-02-16 | GSE32408 | GEO

Expression data from TOP-GFP sorted colon cancer cells

Project description:Colon cancers typically contain tumor cell populations with differential WNT signaling activity. Colon cancer cells with high WNT-activity have been attributed increase tumorigenic potential and stem cell characteristics. We extracted tumor cells with differential WNT activity using fluorescent reporters from xenografted human colon cancer cell line tumors and primary human colon cancer xenografts and analysed their gene expression profiles. Colon cancer cell lines and primary colon cancer xenografts were transduced with lentiviral TOP-GFP reporters, and grown as xenografts in NOD-SCID mice. We disaggregated these tumors, flow sorted for GFPhigh and GFPlow tumor cells and subjected these populations to gene expression profiling.

2012-02-16 | E-GEOD-32408 | biostudies-arrayexpress

Induction of ER stress in HCT116 colon cancer cells

Project description:To investigate the role of p53 and DICER in the induction of ER stress, wildtype, p53 knockout or DICER mutant HCT116 colon cancer cells were treated with the ER stress inducers tunicamycin or brefeldin A for 24 hours. Microarray analysis was used to determine changes in gene expression associated with the induction of ER stress, and to compare this induction in wildtype, p53 knockout or DICER mutant HCT116 colon cancer cells

2015-04-01 | GSE63252 | GEO

Colon cancer

Project description:Colon cancer

| PRJNA300948 | ENA

Abrogation of WNT signaling by dominant-negative TCF4

2015-04-01 | GSE28467 | GEO

Abrogation of WNT signaling by dominant-negative TCF4

Project description:To assess cellular changes upon abrogation of the WNT-signaling pathway, we induced expression of a dominant-negative T-cell factor 4 (TCF4). This showed a remarkable overlap with activation of the unfolded protein response (UTR) in the same colon cancer cell line. 500,000 cells were plated in a 10 cm2 plate, and the following day, treated for 20 hrs with doxycycline (Dox) or vehicle. Three independent biological replicates per treatment.

2015-04-01 | E-GEOD-28467 | biostudies-arrayexpress

Epithelial Wnt secretion drives the progression of inflammation-induced colon carcinoma

Project description:Colon cancer is initiated by stem cells that escape the strict control. In most colon tumors this process is driven through aberrant activation of Wnt signaling by mutations that occur in components acting downstream of the receptor complex and that unfetter tumor cells from the need for Wnt ligands. Here we describe a special type of colon cancer that does not depend on mutated core components of the Wnt pathway. Genetically blocking Wnt secretion from epithelial cells of such tumors results in apoptosis, reduced expression of colon cancer markers, followed by enhanced tumor differentiation. In contrast to the normal colonic epithelium, such tumor cells auto-secrete Wnt ligands to maintain their uncontrolled proliferative behavior. In humans, we determined certain cases of colon cancers in which the Wnt pathway is hyperactive, but not through mutations in its core components. Our findings illuminate the path in therapy to find further subtypes of Wnt- dependent colon cancer, that might be responsive to Wnt secretion inhibitors.

2021-09-01 | GSE181738 | GEO

RNA-seq analyses of autophagy-high and autophagy-low cells from human colon cancer organoids

Project description:We isolated the human colon cancer cells expressing high leveles and low levels of autophagic activity by introdicing an autophagic flux indicator in organoids and performed RNA-seq analyses.

2023-04-23 | GSE193936 | GEO

Transcription profiling of human colon cancer cell lines

Project description:Assess the full impact of estrogen receptor beta on transcription by a full transcriptome analysis of ERb-mediated gene regulation in the two colon cancer cell lines HCT116 and HT29. The colon cancer cell lines do not express endogenous ER but is made ERb-expressing by lentiviral transduction of an ERb expression cassette. Introduction of ERb makes it possible to study the role and function of ERb in colon cancer as well as the impact ERb has on its own (in the absence of ERa).

2011-04-15 | E-MEXP-3169 | biostudies-arrayexpress

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