Transcriptomics,Genomics

Dataset Information

48

Identification of genes regulating TRAIL-induced apoptosis in rheumatoid arthritis fibroblasts-like synoviocytes (RA FLS)


ABSTRACT: Rheumatoid arthritis (RA) is a chronic systemic inflammatory disease involving primarily the synovial membranes and articular structures of multiple joints. A hallmark of RA is the pseudo-tumoral expansion of fibroblast-like synoviocytes (FLS), as these cells invade and finally destroy the joint structure. RA FLS have been therefore proposed as a therapeutic target. > TNF-related apoptosis-inducing ligand (TRAIL) has been described as a pro-apoptotic factor on malignant cells. The fact that fibroblasts-like-synoviocytes (FLS) in rheumatoid arthritis RA patients exhibit tumor like features led us to investigate the effect of TRAIL on ex-vivo RA FLS. We have previously described that TRAIL induces apoptosis only in a subset of RA FLS, but an induction of proliferation in the surviving cells. This observation corresponds to the pleiotropic effects of TRAIL observed on primary human tumor cells. We also observed that sensitivity to TRAIL-induced apoptosis varied in RA FLS from one patient to another, and was correlated with disease severity. We therefore screened for genes that were differentially expressed in RA FLS sensitive and resistant to TRAIL induced apoptosis in order to understand molecular factors making cells resistant or sensitive to TRAIL induced apoptosis. Overall design: The sensitivity of synovial fibroblasts from rheumatoid arthritis patients was defined on the percentage of apoptotic cells following TRAIL stimulation: 0-10% corresponded to resistant cells and above 25% to sensitive RAFLS. We performed transcriptomic comparison between twelve sensitive (n=6) and resistant (n=6) synovial fibroblasts. A loop design and dye swap were used for the comparison of resistant and sensitive RAFLS.

INSTRUMENT(S): MGX_Human_35K_AROSV4.0.2

SUBMITTER: Rachel AUDO  

PROVIDER: GSE65908 | GEO | 2018-02-12

REPOSITORIES: GEO

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Publications

Identification of genes regulating TRAIL-induced apoptosis in rheumatoid arthritis fibroblasts-like synoviocytes.

Audo R R   Hegglin A A   Severac D D   Dantec C C   Combe B B   Hahne M M   Morel J J  

Genes and immunity 20150806 7


We previously described that sensitivity to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis varied in rheumatoid arthritis fibroblasts-like synoviocytes (RAFLS) from one patient to another and was correlated with disease severity. Therefore, we screened for genes differentially expressed in RAFLS sensitive and resistant to TRAIL-induced apoptosis. The sensitivity of RAFLS was defined based on the percentage of TRAIL-induced apoptosis: 0-10% for resistant cells a  ...[more]

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