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Gene expression analysis in differentiated day 3 cells from mouse ESC under the treatment of DMSO and histone deacetylase inhibitors (TSA) [expression array]

ABSTRACT: Based on the cDNA microarrays performed with the RNAs prepared from the differentiated cells at day 3 with or without 24 hrs TSA treatment initiated at day 2, we reveal that the upregulated gene sets were highly enriched for biological functions related to mesoderm development, mesoderm morphogenesis and others.

ORGANISM(S): Mus musculus

PROVIDER: GSE66026 | GEO | 2015/02/19

SECONDARY ACCESSION(S): PRJNA275706

REPOSITORIES: GEO

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Gene expression analysis in differentiated day 3 cells from mouse ESC under the treatment of DMSO and histone deacetylase inhibitors (TSA) [expression array]

Project description:Based on the cDNA microarrays performed with the RNAs prepared from the differentiated cells at day 3 with or without 24 hrs TSA treatment initiated at day 2, we reveal that the upregulated gene sets were highly enriched for biological functions related to mesoderm development, mesoderm morphogenesis and others. Three independent experiments were performed at each treatment (DMSO or TSA).

2015-02-19 | E-GEOD-66026 | biostudies-arrayexpress

Trichostatin A (TSA) inhibition of histone deacetylase in Arabiodopsis thaliana

Project description:Histone acetylation is involved in the regulation of gene expression in plants and eukaryotes. Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from histones, which is associated with the repression of gene expression. To study the role of histone acetylation in the regulation of gene expression during seed germination, trichostatin A (TSA), a specific inhibitor of histone deacetylase, was used to treat imbibing Arabidopsis thaliana seeds. GeneChip arrays were used to show that TSA induces up-regulation of 45 genes and down-regulation of 27 genes during seed germination. Eight TSA-up-regulated genes were selected for further analysis - RAB18, RD29B, ATEM1, HSP70 and four late embryogenesis abundant protein genes (LEA). A gene expression time course shows that these eight genes are expressed at high levels in the dry seed and repressed upon seed imbibition at an exponential rate. In the presence of TSA, the onset of repression of the eight genes is not affected but the final level of repressed expression is elevated. Chromatin immunoprecipitation and HDAC assays show that there is a transient histone deacetylation event during seed germination at one day after imbibition, which serves as a key developmental signal that affects the repression of the eight genes. Keywords: histone deacetylase inhibitor, developmental effects

2005-12-10 | GSE3783 | GEO

Gene expression analysis of mouse testis after treatment with histone deacetylase inhibitor TSA

Project description:Trichostatin A (TSA) is one of the most potent reversible histone deacetylase (HDAC) inhibitors. In male mice, subcutaneous application of TSA is followed by infertility due to apoptosis of pachytene spermatocytes. To get more insight into the mechanisms underlying this phenomenon, we performed a genome-wide expression analysis of murine testes after TSA treatment. The whole genome transcriptome analysis revealed 507 significantly regulated genes. Validation by real-time quantitative PCR confirmed the expression of 7 from 9 genes tested (78%). Keywords: time course

2007-08-02 | GSE5448 | GEO

Transcription profiling of Arabidopsis treated with Trichostatin A (TSA) to inhibit of histone deacetylase in Arabiodopsis thaliana

Project description:Histone acetylation is involved in the regulation of gene expression in plants and eukaryotes. Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from histones, which is associated with the repression of gene expression. To study the role of histone acetylation in the regulation of gene expression during seed germination, trichostatin A (TSA), a specific inhibitor of histone deacetylase, was used to treat imbibing Arabidopsis thaliana seeds. GeneChip arrays were used to show that TSA induces up-regulation of 45 genes and down-regulation of 27 genes during seed germination. Eight TSA-up-regulated genes were selected for further analysis - RAB18, RD29B, ATEM1, HSP70 and four late embryogenesis abundant protein genes (LEA). A gene expression time course shows that these eight genes are expressed at high levels in the dry seed and repressed upon seed imbibition at an exponential rate. In the presence of TSA, the onset of repression of the eight genes is not affected but the final level of repressed expression is elevated. Chromatin immunoprecipitation and HDAC assays show that there is a transient histone deacetylation event during seed germination at one day after imbibition, which serves as a key developmental signal that affects the repression of the eight genes. Experiment Overall Design: Two samples (TSA-treated v.s. untreated). Two replicates for each sample.

2008-06-12 | E-GEOD-3783 | biostudies-arrayexpress

Gene expression analysis of RNAs isolated from TSA-untreated and TSA-treated human keratinocytes

Project description:In order to identify the TSA responsive genes, we performed a gene expression microarray analysis for the RNAs isolated from TSA-untreated and TSA-treated human keratinocytes

2018-12-31 | GSE121902 | GEO

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Project description:TSA CLI Holder

| PRJEB25900 | ENA

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Project description:TSA of P. roqueforti AGO

| PRJEB5143 | ENA

RNA-seq analysis of gene expression patterns responding to TSA treatment during hESC neural differentiation

Project description:We report the differential roles of an HDAC inhibitor-TSA during hESC nerual commitment. In the initiation of hESC differentiation, TSA could inhibit the downregulation of pluripotency genes to maintain pluripotency, whereas in the neural commitment stage, TSA could promote neural gene expression to assist hESC nerual determination. Examination of gene expression patterns in hESCs, day 4 or day 8 differentiated cells without or with TSA treatment

2015-03-11 | E-GEOD-66777 | biostudies-arrayexpress

Gene expression induced by TSA in undifferentiated human primary myoblasts versus terminally differentiated human myotubes

Project description:Gene expression analysis in human skeletal myoblasts (undifferentiated mononucleated cells, cultured in growth medium - 15% FBS) and human skeletal myotubes (pre-formed myotubes, cultured in differentiation medium – 2% horse serum) exposed to the HDACi TSA (50nM) for 24 hours TSA treated undifferentiated human primary myoblasts and terminally differentiated human myotubes

2011-03-01 | E-GEOD-27073 | biostudies-arrayexpress

Gene expression induced by TSA in undifferentiated human primary myoblasts versus terminally differentiated human myotubes

2011-03-01 | GSE27073 | GEO

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