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TGFβ1 treatment of A2058 melanoma cells

ABSTRACT: 4 replicates were prepared from A2058 melanoma cells [transfected with 10ng of empty vector (pcDNA3.1+)] and treated with 5ng/ml TGFβ1 or vehicle control for 24hrs

ORGANISM(S): Homo sapiens

PROVIDER: GSE66114 | GEO | 2015/02/20

SECONDARY ACCESSION(S): PRJNA275900

REPOSITORIES: GEO

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TGFβ1 treatment of A2058 melanoma cells

Project description:4 replicates were prepared from A2058 melanoma cells [transfected with 10ng of empty vector (pcDNA3.1+)] and treated with 5ng/ml TGFβ1 or vehicle control for 24hrs This is the control arm of a larger experiment where cells transfected with a particular expression plasmid were treated with TGFβ1 or control vehicle. The transfection with the expresion plasmid was unsucessful so this empty vector data has been used alone to simply examine the effect of TGFβ1 treatment on A2058 cells.

2015-02-20 | E-GEOD-66114 | biostudies-arrayexpress

Transcriptomic analysis of the effect of CT16 (PAGE5) silencing by RNAi in A2058 melanoma cells

Project description:The cellular gene expression profiles were investigated after CT16 (PAGE5) silencing in CT16 positive A2058 melanoma cells. Control siRNA treated A2058 cells was used as a negative control. Total RNA from three individual cultures of A2058 cells treated with CT16 siRNA and from three individual cultures of A2058 cells treated with control siRNA.

2012-08-09 | E-GEOD-31351 | biostudies-arrayexpress

Transcriptomic analysis of the effect of CT16 (PAGE5) silencing by RNAi in A2058 melanoma cells

2012-08-10 | GSE31351 | GEO

Gene expression in Vemurafenib-treated A375 and A2058 melanoma cell lines

Project description:Human melanoma cell line A375 and A2058 were incubated with DMSO (control group) or Vemurafenib (1 μM in DMSO) for 72 hours. Total RNA was isolated with Qiagen RNA isolation kit; 1.5 μg of total RNA was used as template for cDNA synthesis.

2015-12-02 | GSE52882 | GEO

Transcriptomic analysis of CT16 (PAGE5) function in A2058 and WM-266-4 melanoma cells

Project description:This SuperSeries is composed of the following subset Series: GSE31350: Transcriptomic analysis of the effect of CT16 (PAGE5) expression in WM-266-4 melanoma cells GSE31351: Transcriptomic analysis of the effect of CT16 (PAGE5) silencing by RNAi in A2058 melanoma cells Refer to individual Series

2012-08-09 | E-GEOD-31352 | biostudies-arrayexpress

Single cell RNAseq data from A2058 cell line (Melanoma)

Project description:The goal of this study was to unlock the cellular diversity of A2058, a melanoma cell line known to be resistant to BRAF inhibition, by performing single cell RNAseq profiling using a 10X genomics platform. This approach allowed us to study the transcriptional programming of every single melanoma cell, particularly of those cells expressing ABCB5, a cell surface protein involved in invasiveness and resistance to BRAF inhibition. Findings from this study revelaed that ABCB5-expressing cells also expressed melanocytic genes (MITF) but with a distinct transcriptional program to those expressing AXL. We also used this data to identify the expresion of commonly studied drug efflux pumps in melanoma cells.

2020-06-22 | GSE150241 | GEO

Homo sapiens

Project description:TGFβ1 treatment of A2058 melanoma cells

| PRJNA275900 | ENA

Single cell RNAseq data from A2058 cell line (Melanoma)

Project description:Single cell RNAseq data from A2058 cell line (Melanoma)

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Gene expression profiling in carmustine-resistant melanoma A2058 cells

Project description:Gene expression profiling in carmustine-resistant melanoma A2058 cells

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MKN28 Cells: Vector vs ZIC1 Transfected

Project description:MKN28 cell lines were transfected with pCDNA3.1-ZIC1 or pCDNA3.1 empty vector. Then, an Agilent oligonucleotide microarray was conducted to systematically determine downstream targets of ZIC1.

2012-06-26 | GSE38924 | GEO

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