Genomics

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Comparison of gene expression in human embyronic stem cells grown in MEF-CM or defined media conditions


ABSTRACT: A fully defined media for human embryonic stem cells (hESCs) was designed after examining the phosphorylation status of 42 receptor tyrosine kinases (RTKs) on hESCs exposed to mouse embryonic fibroblast conditioned medium (CM). Activation of the insulin and insulin-like growth factor-1 receptors (IR, IGF1R) as well as EGFR family members including ERBB2 and ERBB3 led to the design of a simple defined media which supported robust long-term growth of multiple hESC lines and allowed massive expansion of undifferentiated cells. Illumina bead arrays were used to demonstrate the maintenance of gene expression profiles characteristic of pluripotent cells in cultures grown in CM or defined media. Keywords: Comparison of cells in different culture conditions

ORGANISM(S): Homo sapiens

PROVIDER: GSE6645 | GEO | 2007/06/24

SECONDARY ACCESSION(S): PRJNA98823

REPOSITORIES: GEO

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