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Insights into responses of human astrocytes to H5N1 infection by transcriptional analysis

ABSTRACT: Increasing evidence demonstrates influenza virus can not only affect the respiratory system, but also infect CNS and lead to CNS disorder and encephalopathy and encephalitis. Astrocytes are the most abundant cells in the CNS, which are capable of producing cytokines and neurotrophic factors and are essential for brain homeostasis and neuronal function. Previous studies suggested that influenza virus can infect astrocytes and induce proinflammatory cytokines response as well as apoptosis. Nevertheless, very few mechanistic data are available regarding host responses to H5N1 infection in astrocytes. In this study, a functional genomics approach was utilized to investigate comprehensive host responses to H5N1 infection in a human astrocyte cell line, U251 cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE66597 | GEO | 2016/01/04

SECONDARY ACCESSION(S): PRJNA277411

REPOSITORIES: GEO

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Insights into responses of human astrocytes to H5N1 infection by transcriptional analysis

Project description:Increasing evidence demonstrates influenza virus can not only affect the respiratory system, but also infect CNS and lead to CNS disorder and encephalopathy and encephalitis. Astrocytes are the most abundant cells in the CNS, which are capable of producing cytokines and neurotrophic factors and are essential for brain homeostasis and neuronal function. Previous studies suggested that influenza virus can infect astrocytes and induce proinflammatory cytokines response as well as apoptosis. Nevertheless, very few mechanistic data are available regarding host responses to H5N1 infection in astrocytes. In this study, a functional genomics approach was utilized to investigate comprehensive host responses to H5N1 infection in a human astrocyte cell line, U251 cells. U251 cells were infected by H5N1 at MOI 1 or control . At time points 6, 12, and 24h, total RNA were extracted for microarray experiment. Three replicates were performed at each time point of infection and control infection.

2016-01-04 | E-GEOD-66597 | biostudies-arrayexpress

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2010-06-16 | E-GEOD-22212 | biostudies-arrayexpress

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Project description:The cellular transcriptome of C57BL/6 mouse lungs was profiled by mRNA-Seq analysis at multiple time points in response to infection with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus. The Influenza A/Vietnam/1203/04 (H5N1) HALo mutant virus is an attenuated H5N1 virus generated from wild-type Influenza A/Vietnam/1203/04 (H5N1) virus as described in Steel, J., et al. J Virol. 2009 Feb; 83(4):1742-53. A/Wyoming/03/03 (H3N2) influenza virus replicates poorly in mice and lung tissue collected from mice inoculated with this virus did not carry viral loads detectable by plaque assay.

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Project description:Periodic outbreaks of highly pathogenic avian H5N1 influenza viruses and the current H1N1 pandemic highlight the need for a more detailed understanding of influenza virus pathogenesis. To investigate the host transcriptional response induced by pathogenic influenza viruses, we used a functional-genomics approach to compare gene expression profiles in lungs from wild-type 129S6/SvEv and interferon receptor (IFNR) knockout mice infected with either the fully reconstructed H1N1 1918 pandemic virus (1918) or the highly pathogenic avian H5N1 virus Vietnam/1203/04 (VN/1203).

2013-07-16 | GSE48890 | GEO

Genome wide host gene expression analysis in duck lungs infected with highly or low pathogenic H5N1 avian influenza virus

Project description:The underlying molecular mechanisms of pathogenesis and outcome of disease to different pathotypes of H5N1 influenza infection in ducks remain unclear. For that, we studied genome wide host gene expression of lung tissues infected with A/duck/India/02CA10/2011(AD2011) H5N1 virus and A/duck/Tripura/103597/2008 (AD2008) H5N1 virus in ducks using custom designed microarray. AD2011 is highly pathogenic whereas AD2008 is low pathogenic to ducks. Comparative analysis of differentially expressed genes revealed that 688 genes were commonly expressed, 877 and 1556 genes are uniquely expressed to infection with AD2011 and AD2008 virus isolate, respectively. The up-regulation of cytokines genes OAS, IL1B, IL17, IFITM2, CCL4, CXCR4, STAT3, TGFB1 and TGFB2 in the lungs tissues may cause high mortality in ducks infected with AD2011 virus. The expression of important antiviral immune genes IFIT5, IFITM5, RSAD2, EIF2AK2 (PKR), Mx, β-defensins, TRIM23 and SLC16A3 to AD2008 infection, but not in AD2011 infection, cause the host may fine-tune their innate immune responses and prevent from cytokines storms and tissue damage. Several immune related Gene ontology (GO) terms and immune pathways activated were qualitatively similar but quantitatively different to both virus infections. Based on these findings, we conclude that subtle differences in host immune responses may determine the different outcome of H5N1 infection in ducks.

2016-01-02 | GSE65230 | GEO

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