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Temperature-dependent regulation of gene expression in Japanese flounder Paralichthys olivaceus treated with poly (I:C)

ABSTRACT: 253 genes showed more than 4-fold up- or down-regulated expressions by poly (I:C) at 15 ºC and 25 ºC.

ORGANISM(S): Paralichthys olivaceus

PROVIDER: GSE66692 | GEO | 2015/09/08

SECONDARY ACCESSION(S): PRJNA277732

REPOSITORIES: GEO

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Temperature-dependent regulation of gene expression in Japanese flounder Paralichthys olivaceus treated with poly (I:C)

Project description:253 genes showed more than 4-fold up- or down-regulated expressions by poly (I:C) at 15 ºC and 25 ºC. Fish were treated with poly(I:C) and reared at 15 ºC and 25 ºC and isolation of total RNA from spleen of the fish at 3 and 24 hours after the treatment.

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Project description:In previous study, we revealed that IRF10 is involved in the immune-reponse against bacteria and virus in Paralichthys olivaceus. However, the target genes of IRF10 is still unknown in teleosts. We searched the target genes of IRF10 by microarray in IRF10 over expressed HINAE cell HINAE cells were culured in 24-well plate. HINAE cells were transfected using lipofectamine2000 (Invitrogen, USA) with 7.2nM IRF10 expression plasmid vector (pcDNA3.1-IRF10) or empty-vector (pcDNA3.1). The equal volume of sterile water was used as a control. On other hands, the 1μg/ml of poly I:C also transfected to HINAE cells using lipofectamine2000 with each plasmid and vehicles (sterile water). After 48 hours from the transfection, we harvested the two samples of the control and poly I:C only and the three samples of plasmids or plasmids with poly I:C transfected cells (16 hybridization).

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