Transcriptomics,Genomics

Dataset Information

42

Development of coregulator-dependent androgen receptor target gene signatures


ABSTRACT: To decipher the contribution of coregulators to androgen receptor target gene expression, effect of siRNA-mediated silencing of 18 clinically relevant coregulators on bona fide androgen receptor target gene expression was studied. Overall design: Human LNCaP prostate cancer cells were transfected with individual siRNA SmartPools targeting 18 clinically relevant coregulators or a non-targeting siRNA SmartPool. Forty-two hours after transfection, cells were treated with synthetic androgen R1881 (5nM) or vehicle. Three biological replicates were generated per treatment group. Forty-eight hours later, total RNA was isolated and processed for Agilent microarray analysis. The two normalizer are aliquots from same RNA sample that serves as internal control.

INSTRUMENT(S): Agilent-029218 Heemers AR axis PCF 2010

SUBMITTER: Dan Wang  

PROVIDER: GSE66722 | GEO | 2016-02-19

SECONDARY ACCESSION(S): PRJNA277808

REPOSITORIES: GEO

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