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Genome-wide analysis of LPS or PBS challenged DUSP3-KO and WT female mice peritoneal macrophages gene expression

ABSTRACT: Analysis of gene expression profile in peritoneal macrophage extracted from LPS or PBS challenged DUSP3-/- and WT mice. DUSP3 deletion protects mice from sepsis and endotoxemia. We performed a microarray analysis to get insights into the differentially regulated pathways between WT and KO under inflammatory conditions.

ORGANISM(S): Mus musculus

PROVIDER: GSE66782 | GEO | 2015/03/11

SECONDARY ACCESSION(S): PRJNA277953

REPOSITORIES: GEO

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Genome-wide analysis of LPS or PBS challenged DUSP3-KO and WT female mice peritoneal macrophages gene expression

Project description:Analysis of gene expression profile in peritoneal macrophage extracted from LPS or PBS challenged DUSP3-/- and WT mice. DUSP3 deletion protects mice from sepsis and endotoxemia. We performed a microarray analysis to get insights into the differentially regulated pathways between WT and KO under inflammatory conditions. Total RNA obtained from isolated peritoneal macrophages isolated from DUSP3-/- and WT mice 36 hours after challenging them in vivo with PBS or with 6mg/kg of LPS.

2015-03-11 | E-GEOD-66782 | biostudies-arrayexpress

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Project description:To investigate the effect of Bmal1 on poly(I:C) response, we isolate mRNA from PBS or poly(I:C)-injected WT and myeloid Bmal1 KO mice. We then compare the poly(I:C)-induced transcriptomic change between WT and Bmal1 KO peritoneal myeloid cells.

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LPS-stimulated endothelial cells using SILAC

Project description:Severe sepsis and septic shock, which occur with high incidence and are prevalent in the emergency department setting, are among the most common causes of death in hospitalized patients worldwide, and there has been a continuous increase in the incidence of sepsis of approximately 5–10% per year. Endothelial cells constitute an important metabolic organ in the physiological homeostasis of blood vessels and play a pivotal role in orchestrating the inflammatory response triggered by sepsis or endotoxemia. The secretome is defined as the subset of the proteome that contains all the proteins actively exported by a cell following a particular event.

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2024-02-18 | GSE230030 | GEO

Casp8- and Casp11-dependent gene expression in ileal mucosa of LPS-challenged male mice

Project description:The execution of shock following high dose E. coli lipopolysaccharide (LPS) or bacterial sepsis in mice required pro-apoptotic caspase-8 in addition to pro-pyroptotic caspase-11 and gasdermin d. Hematopoietic cells produced MyD88- and TRIF-dependent inflammatory cytokines sufficient to initiate LPS shock independent of caspase-8 or caspase-11. Both proteases had to be present to support tissue injury, dependent upon TNF and interferon , first observed in the small intestine and later in spleen and thymus. Caspase-11 enhanced the activation of caspase-8 and extrinsic cell death machinery within the lower small intestine. Neither caspase-8 nor caspase-11 was individually sufficient for shock, but collaborated to amplify the inflammatory signals associated with tissue injury. Therefore, combined pyroptotic and apoptotic signaling mediated endotoxemia independent of RIPK1 kinase activity and RIPK3 function. These observations bring to light the relevance of tissue compartmentalization during inflammatory signaling in vivo where cytokines execute diverse cell death pathways and tissue damage.

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LPS-stimulated endothelial cells using SILAC

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