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Gene expression in Rhagoletis pomonella and R. zephyria transplanted into snowberry and apple fruits

ABSTRACT: Gene expression in feeding larvae reciprocally transplanted into snowberry and apple fruits

ORGANISM(S): Rhagoletis zephyria Rhagoletis pomonella

PROVIDER: GSE66975 | GEO | 2015/10/01

SECONDARY ACCESSION(S): PRJNA278596

REPOSITORIES: GEO

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Gene expression in Rhagoletis pomonella and R. zephyria transplanted into snowberry and apple fruits

Project description:Gene expression in Rhagoletis pomonella and R. zephyria transplanted into snowberry and apple fruits

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Identification of differentially methylated regions between leaves and young fruits of the apple GDDH13

Project description:The main objective of this analysis was to sequence the epigenome of the Apple (Malus domestica) doubled haploid 'Golden Delicious' tree. Our secondary objective was to identify differentially methylated regions between DNA purified from leaves and young fruits.

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Project description:Based on sensorial analysis over 4 years, 6 apple genotypes with contrasted fruit texture (mealy or not) were selected among a progeny. Apple samples were collected at 100 days after flowering (100 DAF), harvest (H), after 2 and 4 months of cold storage (60DAH and 120DAH respectively). 6 apple hybrids were analysed in dye-switch. Biological replicates are fruits from 2 to 4 different harvest years. Each mealy hybrid was compared to a non-mealy hybrid from the same harvest year in 12 dye-swap 3 pairs at 4 four time points).

2015-06-25 | E-GEOD-59947 | biostudies-arrayexpress

Transcriptomic profiling of apple seeds

Project description:Comparison of seed of two different types of developing fruits in apple: central and lateral. Our objective is to find transcriptomic signatures that allow to explain the physiological drop of young lateral fruitlets Apple seeds transcriptomes were generated by deep sequencing by triplicate from seeds collected from central and lateral fruitlets at 20 days after petal fall (DAPF)

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Transcriptomic profiling of apple seeds

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Global gene expression analysis of resistant and susceptible apple cultivars challenged with Penicillium expansum

Project description:Blue mold, caused by Penicillium expansum, is responsible for postharvest losses of apple fruit, and threatens human health through production of the potent mycotoxin patulin. No major gene(s) providing resistance have as yet been identified, but recent studies indicate a quantitative control of the disease. An AryANE chip covering 60K apple transcripts was used to identify possible candidate gene(s) that are differentially regulated between resistant and susceptible cultivars upon P. expansum infection. Induction of cell wall related gene (PGIP1), and three genes involved in the â€˜down-streamâ€™ flavonoid biosynthesis pathway (CHS, FLS and LDOX), shows the fundamental role of cell wall as an important barrier, and contents of polyphenolic compounds of fruits as a quantitative components in enhancing disease resistance to blue mold. Moreover, exogenous application of Jasmonic acid hormone enhanced the defense mechanism in fruits. This is the first report linking Jasmonic acid and activation of cell wall and flavonoid pathway genes in apple fruit resistance to blue mold. Results provide an initial categorization of genes that are potentially involved in the resistance mechanism, and should be useful for developing tools for gene marker-assisted breeding of apple cultivars with an improved resistance to blue mold. SUBMITTER_CITATION: Ahmadi-Afzadi, M., Orsel Baldwin, M., Pelletier, S., Cournol, M., Proux-Wéra, E., Nybom, H., Renou, J.-P. (2018). Genome-wide expression analysis suggests a role for jasmonates in the resistance to blue mold in apple. Plant Growth Regulation, 85 (3), 375-387. , DOI : 10.1007/s10725-018-0388-2

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Transcriptional Profiling of Apple (Malus x domestica Borkh) Floral Induction in Shoot Apical Meristems

Project description:Effect of the presence of fruits on the expression of genes possibly involved in floral induction in the terminal meristem of spur bourse shoot. Investigation on mecanisms involved in Biennial Bearing in mature apple trees cultivar Royal Gala.

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Ontogenic Resistance in Apple

Project description:Ontogenic scab resistance in apple leaves and fruits is a horizontal resistance against the plant pathogen VenturiaM- inaequalis and is expressed as decrease of disease symptoms and incidence with the ageing of the leaves. Several studies at biochemical level tried to unveil the nature of this resistance, however without any conclusive results. We decided therefore to investigate the genetic origin of this phenomenon by performing a full quantitative trascriptome sequencing and comparison of young (susceptible) and old (ontogenic resistant) leaves, infected or not with the pathogen. Two time points at 72 and 96 hours post inoculation were chosen for RNA sampling and sequencing. Comparison between the different conditions (young and old leaves, inoculated or not) should allow finding genes differentially expressed which may represent different induced plant defense reaction leading to ontogenic resistance or be the cause for a constitutive (not inoculated with the pathogen) shift toward resistance in old leaves. Differentially expressed genes were then characterized for their function by homology to A.M- thaliana and other plantsM-^R genes, particularly looking for genes involved in pathways already suspected of appertaining to ontogenic resistance in apple or other hosts, or to plant defense mechanisms in general.

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