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Age-dependent Response of Isolated Male Germ Cells to Oxidative Stress

ABSTRACT: Male germ cells from young and aged Rats were Isolated and cultured and then treated with pro-oxidant SIN-1 and antioxidant EUK134 Study of the response of isolated male germ cells from young and aged Brown Norway Rats to oxidative stress.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE66976 | GEO | 2015/04/30

SECONDARY ACCESSION(S): PRJNA278622

REPOSITORIES: GEO

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Age-dependent Response of Isolated Male Germ Cells to Oxidative Stress

Project description:Male germ cells from young and aged Rats were Isolated and cultured and then treated with pro-oxidant SIN-1 and antioxidant EUK134 Study of the response of isolated male germ cells from young and aged Brown Norway Rats to oxidative stress. Treatment of Isolated and cultured germ cells with pro-oxidant and antioxidant.

2015-04-30 | E-GEOD-66976 | biostudies-arrayexpress

Expression data of pachytene spermatocytes and round spermatids from young and aged Brown Norway rats

Project description:Male fertility and testis function changes with age and so it was sought to determine if these changes are accompanied by changes in gene expression. A full genome microarray was used to determine if distinct pathways of genes were altered in expression in germ cells (pachytene spermatocytes or round spermatids) with age. PACH: Testes from young (4 months) and aged (18 months) Brown Norway rats were subjected to a density gradient cell separation to isolate pachytene spermatocytes. RNA was isolated from these cells for hybridization on affymetrix miroarrays. ROU: Testes from young (4 months) and aged (18 months) Brown Norway rats were subjected to a density gradient cell separation to isolate round spermatids. RNA was isolated from these cells for hybridization on affymetrix miroarrays.

2012-01-06 | E-GEOD-29963 | biostudies-arrayexpress

Single-nucleus transcriptomic sequencing of aging hypothalamus treated with 17a-estradiol

Project description:To dissect the role of 17a-estradiol in lifespan extending and its potential side effects for long-term administration, the pooled hypothalami from aging male Norway brown rats treated with 17a-estradiol for 6 months (O.T), aged male control (O), and young male control (Y) were subjected to single-nucleus transcriptomic sequencing (snRNA-seq).

2024-05-01 | GSE248413 | GEO

Expression data of CD9 positive germ cells from young and aged Sprague Dawley germ cell specific GFP rats

Project description:Male fertility and testis function changes with age and so it was sought to determine if these changes are accompanied by changes in gene expression in the spermatogonial cells at the foundation of spermatogenesis A full genome microarray was used to determine if distinct pathways of genes were altered in expression in CD9-positive germ cells with age Testes from young (4 months) and aged (21 months) Sprague Dawley rats were subjected to dissociation to isolate a singel cell suspension of germ cells. These cells were used to isolate CD9 positive spermatogonial cells. RNA was isolated from these cells for hybridization on agilent miroarrays.

2013-12-05 | E-GEOD-50143 | biostudies-arrayexpress

Differential proteomics of the synaptosomal fraction from rat dentate gyrus identified the changes associated with aging-dependent cognitive decline

Project description:The study contained three groups of Sprague-Dawley male rats – two groups of aging animals (22-24 months) separated based on performance in spatial reference memory task; and the third, control group of young animals () not separated by behavioural testing. The synaptosome – enriched membrane fraction of the dentate gyrus was isolated from nine randomly chosen individuals per group and analysed by tandem mass tag (TMT) labelling and mass spectrometry (MS)-based quantitative proteomics. The proteomic experiment was performed in three TMT10plex sets, with each set containing samples from three biological replicates per group and one TMT 126 reference channel reserved to directly compare the protein intensities across the entire study. The peptides were analysed in two parallel MS runs representing two technical replicates per biological sample. The total number of 6513 protein entries was identified of which 471 showed a significantly changed level (p<0.05) in any of the three animal groups. The comparison between aged and young animals revealed 293 significantly changed proteins (p<0.05) in aged good performers vs young rats, and 310 significant proteins (p < 0.05) in aged bad performers vs young ones. The proteomic differences between aged good vs bad performers were represented by 78 significantly changed proteins (p < 0.05).

2019-08-19 | PXD013172 | Pride

Transcription profiling of rat dentate gyrus of the hippocampus from young and aged rats to identification of putative learning and memory genes following the Morris Water Maze

Project description:The aim of this study was to investigate whether the differences in memory decline associated with aging are a result of differences in gene expression. We first categorized age-unimpaired and age-impaired rats based on their performance in the Morris water maze, when compared to young rats. Then we isolated messenger RNA from the dentate gyrus of the hippocampus of each animal to interrogate Affymetrix RAE 230A rat genome microarrays. Supervised statistical analysis of the different groups of aged animals recognized 85 genes (p<0.005) that were significantly different in the dentate gyrus of aged rats that had learned the Morris water maze (MWM) paradigm when compared to learning impaired and a number of controls for stress, exercise and non-spatial learning. In addition, statistical analysis of the microarray data that included young and aged rats identified 1129 genes (p<0.005) that were differentially expressed between aged and young rats independent of behavior, but due to aging alone. Experiment Overall Design: a total of 80 samples were analyzed including aged and young rats for aged versus young comparisons. One chip was interrogated per animal. Supervised analysis of aged rat data (aged unimpaired (HID U) versus controls : HID I, VIS, YOKE, SIT includes 39 samples (intermediate learners were not included in the analysis). Controls include cage controls, yoke controls (no platform), visible platform controls.

2008-06-13 | E-GEOD-4821 | biostudies-arrayexpress

Identification of putative learning and memory genes in the dentate gyrus of aged rats following the Morris Water Maze

2007-07-31 | GSE4821 | GEO

Transcription profiling of male rats Brown Norway and F344 reveals coordinated changes in xenobiotic metabolizing enzyme gene expression in aging

Project description:In order to gain insight into the effects of aging on susceptibility to environmental toxins, we characterized the expression of xenobiotic metabolizing enzymes (XMEs) from the livers of male Brown Norway and F344 rats across the adult lifespan. To examine metabolic processes across lifespan after challenge with a xenobiotic compound, Brown Norway rats were exposed to 1.0 g/kg body weight toluene by oral gavage in corn oil (4ml/kg body weight) or corn oil alone. Experiment Overall Design: Brown Norway rats: Analysis of gene expression profiles for XMEs in male Brown Norway rats 4, 12, and 24 months old. Rats were exposed to 1g/kg toluene by oral gavage, and sacrificed after 4 hours. Total RNA was isolated from liver samples and gene expression analyzed using Affymetrix Rat 230 2.0 full-genome GeneChips. Data from 18 samples, with three rats in each of the control age groups and dosed age groups, were analyzed. Experiment Overall Design: F344 rats: Analysis of gene expression profiles for XMEs in male F344 6, 11, 18, and 24 months old. Total RNA was isolated from liver samples and gene expression analyzed using Affymetrix Rat 230 2.0 full-genome GeneChips. Data from 16 samples, with four rats in each of the 4 age groups, were analyzed.

2008-10-20 | E-GEOD-11097 | biostudies-arrayexpress

Effect of short-term high fat diet consumption on hippocampal and amygdalar gene expression in adult and aged male rats

Project description:It is known that short-term high fat diet consumption leads to significant memory impairments and an exaggerated proinflammatory phenotype in the aged rat brain. To gain a better understanding of the underlying mechanisms driving this cognitive and proinflammatory response, we conducted bulk RNAseq analysis of the hippocampus and amygdala from young adult and aged male rats.

2023-02-15 | GSE223802 | GEO

Transcription profiling of rat hippocampus samples from animals aged 4, 14 and 24 months

Project description:Male Fischer 344 rats aged 4 months (young, n=10), 14 months (mid-aged, n=10), and 24 months (aged, n=10) were trained sequentially on two tasks: Morris Spatial Water Maze (SWM) and Object Memory Task (OMT). The training/testing sequence lasted 7 d, and hippocampal tissue was collected 24 hr later. Training and testing occured on each day except for days 2 and 3 of the 7 d sequence. (01/10/05: Series was updated to correct mislabeling of all sample signal values within the Young Treatment Group)

2007-10-12 | E-GEOD-854 | biostudies-arrayexpress

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