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4 mutants-Transcriptome sup62 and cpsf30 mpk4

ABSTRACT: AU13_15_sup62 - 4 mutants - Transcriptome analysis of the mutant sup62, cpsf30, two suppressors of programmed cell death in A. thaliana - Plants Col-0, mips1, sup62 and sup62 mips1 were grown for 3 weeks in Short-Day condition and subsequently transfered in Long-Day condition. Rosette leaves were sampled after 4 days in Long-Day condition.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE67010 | GEO | 2015/04/01

SECONDARY ACCESSION(S): PRJNA278715

REPOSITORIES: GEO

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4 mutants-Transcriptome sup62 and cpsf30 mpk4

Project description:au13-05_sup62 - 4 mutants - Transcriptome analysis of the mutant sup62, cpsf30, two suppressors of programmed cell death in A. thaliana - Plants Col-0, mips1, sup62 and sup62 mips1 were grown for 3 weeks in Short-Day condition and subsequently transfered in Long-Day condition. Rosette leaves were sampled after 4 days in Long-Day condition. 8 dye-swap - gene knock out,genotype comparaison

2015-04-01 | E-GEOD-67010 | biostudies-arrayexpress

cpsf30 mips1 transcriptome-Cell death control by CPSF30

Project description:au12-06_cpsf30 - cpsf30 mips1 transcriptome - How a mutation in the CPSF30 is able to suppress the cell death phenotype observed in the mips1-1 Arabidopsis mutant? - All the plants were cultivated in the same hanging conditions 10days in "short Days" then in long Days. The takings were later made 4days in "long Days" what corresponds to the appearance of the symptoms of cellular death at the mutant mips1-1. On the other hand, the gene CPSF30 codes for two transcribed. We have a double mutant mips1 cpsf30 complemented with the transcribed young.

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cpsf30 mips1 transcriptome-Cell death control by CPSF30

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Illumina Sequencing of Wild Type, fip37L, cpsf30-3 and cpsf30-1 mutant plants

Project description:The goals of this study are to compare gene readthrough transcription in various plant lines, including a Wild Type control, the fip37L, cpsf30-3 and cpsf30-1 mutant plants.

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The m6A reader CPSF30-L regulates alternative polyadenylation affecting nitrate signaling in Arabidopsis

Project description:As the most ubiquitous internal modification of eukaryotic mRNA, m6A (N6-methyladenosine) modification plays a vital role in almost every aspect of mRNA metabolism. However, the evidence of m6A in regulating the alternative polyadenylation (APA) is limited. APA is controlled by a large protein-RNA complex with many components including CPSF30. Arabidopsis CPSF30 has two isoforms, the longer one named CPSF30-L containing an additional YTH (YT512-B Homology)-domain in the C terminus, which is unique in plant. In this study, we revealed that m6A modification directly regulated APA, by proving the capability of Arabidopsis CPSF30-L YTH domain binding to m6A substrate by using in vitro assay and structural studies. We observed that extensive genes shifted their poly(A) sites in cpsf30-2 and found that numerous genes altered polyadenylation site (PAS) that correlated well with previously identified m6A peaks, indicating that genes carry m6A modification are prone to be regulated by APA. Moreover, we found that several important genes involved in nitrate metabolism are amongst those genes with APA site alteration in cpsf30-2.And we could rescue these APA and nitrate metabolism defects by introducing the wild-type CPSF30-L, but not by m6A-binding defective mutants (W259A, W310A or Y319A), which explained the nitrogen signaling defects of cpsf30-2 discovered previously. Taken together, our results demonstrated that m6A modification could directly regulate APA in Arabidopsis and revealed the function of m6A reader CPSF30-L in nitrate signaling by controlling APA regulation. This study will shed new lights on the roles of m6A modification during RNA 3’-end processing in plant development.

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Project description:Illumina Sequencing of Wild Type, fip37L, cpsf30-3 and cpsf30-1 mutant plants

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Illumina Sequencing of Wild Type, nerd-1 and cpsf30-1 mutant plants

Project description:The goals of this study are to compare plant transcriptome profiling (RNA-seq) obtained from various plant lines, including a Wild Type control, the nerd-1 mutant , and the cpsf30-1 mutant plants.

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RNAseq for atino80 mutant in long-day condition

Project description:RNAseq for atino80 mutant in long-day condition

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atnar2.1-1-Atnar2.1-1 response to transfer on low nitrate condition

Project description:ra05-12_nar2 - atnar2.1-1 - Does the ko of AtNAR2.1 leads to a differential expression in comparison to the WT when grown under high nitrate condition and transfered onto low nitrate concentration? - Plants were grown for 41 days in hydroponics in media containing 6 mM nitrate and then transferred to 6 or 0.2 mM nitrate media for 24h ( short days, irradiation 150 uE). Plants were harvested on day 42 during the first 2 hours of light. Keywords: dose response,gene knock out

2008-03-12 | GSE10705 | GEO

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Project description:au10-07_mpk4gof - mpk4/mpk4 gof vs mpk4/mpk4 vs col0 - Genes regulated by MPK4 pathway. - In order to study of the genes controlled by MPK4 pathway we generate mpk4 complemented lines expressing active MPK4 (MPK4 GOF). For the transcriptome analysis we compare the MPK4 GOF to mpk4/MPK4 (complemented mpk4 mutant with a wt MPK4) and to Col0. Keywords: genotype comparaison

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