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Stem cell and neurogenic gene-expression profiles link prostate basal cells to aggressive prostate cancer

ABSTRACT: The prostate gland mainly contains basal and luminal cells constructed as a pseudostratified epithelium. Annotation of prostate epithelial transcriptomes provides a foundation for discoveries that can impact disease understanding and treatment. Here, we describe a whole-genome transcriptome analysis of human benign prostatic basal and luminal populations by using deep RNA sequencing. Combined with comprehensive molecular and biological characterizations, we show that the differential gene expression profiles account for their distinct functional phenotypes. Strikingly, in contrast to luminal cells, basal cells preferentially express gene categories associated with stem cells, neural and neuronal development and RNA processing. Consistent with their expression profiles, basal cells functionally exhibit intrinsic stem-like and proneural properties with enhanced ribosome RNA (rRNA) transcription activity. Of clinical relevance, the treatment failed castration-resistant and anaplastic prostate cancers molecularly resemble a basal-like phenotype. Therefore, we link the cell-type specific gene signatures to subtypes of prostate cancer development, and identify genes associated with patient clinical outcome.

ORGANISM(S): Homo sapiens

PROVIDER: GSE67070 | GEO | 2015/04/30

SECONDARY ACCESSION(S): PRJNA278856

REPOSITORIES: GEO

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Transcriptome profiling links intrinsic stem cell and proneural properties of basal epithelial cells to human prostate tumorigenesis

Project description:The prostate gland mainly contains basal and luminal cells constructed as a pseudostratified epithelium. Annotation of prostate epithelial transcriptomes provides a foundation for discoveries that can impact disease understanding and treatment. Here, we describe a whole-genome transcriptome analysis of human benign prostatic basal and luminal populations by using deep RNA sequencing. Combined with comprehensive molecular and biological characterizations, we show that the differential gene expression profiles account for their distinct functional phenotypes. Strikingly, in contrast to luminal cells, basal cells preferentially express gene categories associated with stem cells, neural and neuronal development and RNA processing. Consistent with their expression profiles, basal cells functionally exhibit intrinsic stem-like and proneural properties with enhanced ribosome RNA (rRNA) transcription activity. Of clinical relevance, the treatment failed castration-resistant and anaplastic prostate cancers molecularly resemble a basal-like phenotype. Therefore, we link the cell-type specific gene signatures to subtypes of prostate cancer development, and identify genes associated with patient clinical outcome. Human total RNA profiles of 3 pairs of benign prostatic basal and luminal populations freshly purified from prostate tissues of three prostate cancer patients by deep RNA-seq.

2015-04-30 | E-GEOD-67070 | biostudies-arrayexpress

Lineage analysis of basal epithelial cells reveals their unexpected plasticity and supports a cell of origin model for prostate cancer heterogeneity

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Gene expression profiles of Notch signaling suppression in prostate basal cells.

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2012-11-08 | E-GEOD-34067 | biostudies-arrayexpress

Gene expression profiles of Notch signaling suppression in prostate basal cells.

2012-11-08 | GSE34067 | GEO

Microarray expression profiling data for sorted prostate epithelial subpopulations based on LY6D

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Lineage analysis of basal epithelial cells reveals their unexpected plasticity and supports a cell of origin model for prostate cancer heterogeneity

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Reduced Representation Bisulphite Sequencing on FACS purified cell subpopulations of human prostate cancers and normal tissues.

Project description:Prostate adenocarcinomas contain a predominance of cells with luminal features that display aberrant DNA methylation patterns with useful diagnostic and prognostic potential. However, the DNA methylation features of basal and luminal cells in both normal and cancer tissues remain largely unknown. We have generated genome-wide DNA methylation profiles for FACS purified basal and luminal prostate tumour cells, obtained from radical prostatectomy samples and their paired normal counterparts isolated from unaffected regions in the same patient’s gland.

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Expression data from fractionated prostate epithelial cells (fetal and adult)

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