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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and rea1 mutant total and polysome-bound Transcriptomes

ABSTRACT: Analysis of gene expression level. The hypothesis tested in the present study was that rea1 mutant influence the translation level of translation and nucleosome assembly associated genes. Results provide important information of the gene translation level regulation of ribosome proteins, elongation factors, histones and genes associated with other biological processes.

ORGANISM(S): Zea mays

PROVIDER: GSE67083 | GEO | 2015/04/25

SECONDARY ACCESSION(S): PRJNA278871

REPOSITORIES: GEO

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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and rea1 mutant total and polysome-bound Transcriptomes

Project description:Analysis of gene expression level. The hypothesis tested in the present study was that rea1 mutant influence the translation level of translation and nucleosome assembly associated genes. Results provide important information of the gene translation level regulation of ribosome proteins, elongation factors, histones and genes associated with other biological processes. Endosperm total and polysome-bound mRNA profiles of 15DAP wild type (WT) and rea1 mutant were generated by deep sequencing, in triplicate, using Illumina Hiseq 2500 (Zea mays).

2015-04-25 | E-GEOD-67083 | biostudies-arrayexpress

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and rea1 mutant Transcriptomes

Project description:Analysis of gene expression level. The hypothesis tested in the present study was that rea1 mutant influence the expression of translation and nucleosome assembly associated genes. Results provide important information of the gene expression level regulation of ribosome proteins, elongation factors, histones and genes associated with other biological processes. Endosperm mRNA profiles of 15DAP wild type (WT) and rea1 mutant were generated by deep sequencing, in triplicate, using Illumina Hiseq 2500 (Zea mays).

2015-04-25 | E-GEOD-67080 | biostudies-arrayexpress

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and rea1 mutant Transcriptomes

2015-04-25 | GSE67080 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and dek35 mutant Transcriptomes

Project description:Analysis of gene expression level. The hypothesis tested in the present study was that dek35 mutant influence the expression of mitochondrial oxidative phosphorylation associated genes. Results provide important information of the gene expression level regulation of mitochondrial complexes assembly proteins, translational elongation factors and genes associated with other biological processes. Endosperm mRNA profiles of 18DAP wild type (WT) and dek35 mutant were generated by deep sequencing, in triplicate, using Illumina Hiseq 2500 (Zea mays).

2016-03-25 | E-GEOD-79583 | biostudies-arrayexpress

Polysome-bound mRNA during oocyte maturation

2012-01-14 | GSE35106 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and dek35 mutant Transcriptomes

2016-03-25 | GSE79583 | GEO

Radiation-induced alternative transcripts as detected in total and polysome-bound mRNA.

Project description: Not available

| S-EPMC5787501 | biostudies-literature

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and dek10 mutant Transcriptomes

Project description:Analysis of gene expression level. The hypothesis tested in the present study was that dek10 mutant influences the expression of mitochondrial ETC associated genes. Results provide important information of the gene expression level regulation of mitochondrial envelop, proton transport,translation and genes associated with other biological processes.

2016-05-01 | GSE80091 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and dek44 mutant Transcriptomes

Project description:Analysis of gene expression level. The hypothesis tested in the present study was that dek44 mutant influence the expression of mitochondrial ETC associated genes. Results provide important information of the gene expression level regulation of mitochondrial envelop, proton transport,translation and genes associated with other biological processes.

2019-02-23 | GSE126949 | GEO

Polysome-bound mRNA during oocyte maturation

Project description:Oocyte maturation, fertilization, and early embryonic development occur in the absence of gene transcription. Therefore, it is critical to understand at a global level the post-transcriptional events that are driving these transitions. Here, we have used a systems approach by combining polysome mRNA profiling and bioinformatics to identify RNA binding motifs in mRNAs that either enter or exit the polysome pool during mouse oocyte maturation. Association of mRNA with the polysomes correlates with active translation. Forty-eight hours (h) after PMSG injection, mice were stimulated with hCG for 0, 4, or 14 h, and GV-, MI- and MII-stage oocytes were collected. Polysome-bound mRNAs were purified, reverse-transcribed and linearly amplified with the WT-Ovation FFPE RNA Amplification System V2 (NuGEN). 5µg cDNA were fragmented and hybridized with Affymetrix Mouse Genome 430.2 array chips. Experiments were done using 3 independent sample sets.

2012-01-14 | E-GEOD-35106 | biostudies-arrayexpress

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