Cardiac differentiation time-courses of human ES cells using distinct initial induction conditions
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE67154 | GEO | 2015/11/13
SECONDARY ACCESSION(S): PRJNA279187
REPOSITORIES: GEO
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Project description:Efficient directed cardiac induction of human embryonic stem cells requires an intermediate inhibition of WNT signaling, shortly after the initial induction of mesoderm. To reveal the significance of the WNT inhibition step during differentiation, two differential time-course analyses were performed - both comparing WNT-inhibited and non-WNT-inhibited samples: In the first time-course, the WNT inhibitor IWP-2 was either added or omitted on days 2 and 3, and in the second time-course, IWP-2 was either added or omitted on days 1 and 2.
2015-11-13 | GSE67152 | GEO
Project description:Bone morphogenetic protein (BMP) signaling is known to support differentiation of human embryonic stem cells (hESCs) into mesoderm and extraembryonic lineages, whereas other signaling pathways can largely influence this lineage specification. Here, we set out to reinvestigate the influence of ACTIVIN/NODAL and fibroblast growth factor (FGF) pathways on the lineage choices made by hESCs during BMP4-driven differentiation. We show that BMP activation, coupled with inhibition of both ACTIVIN/NODAL and FGF signaling, induces differentiation of hESCs, specifically to βhCG hormone-secreting multinucleated syncytiotrophoblast and does not support induction of embryonic and extraembryonic lineages, extravillous trophoblast, and primitive endoderm. It has been previously reported that FGF2 can switch BMP4-induced hESC differentiation outcome to mesendoderm. Here, we show that FGF inhibition alone, or in combination with either ACTIVIN/NODAL inhibition or BMP activation, supports hESC differentiation to hCG-secreting syncytiotrophoblast. We show that the inhibition of the FGF pathway acts as a key in directing BMP4-mediated hESC differentiation to syncytiotrophoblast.
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