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Expression analysis of big neurospheres generated in vitro

ABSTRACT: Expression analysis of neurospheres generated in vitro treated by potassium chloride (KCl) and norepinephrine

ORGANISM(S): Mus musculus

PROVIDER: GSE67304 | GEO | 2015/06/12

SECONDARY ACCESSION(S): PRJNA279463

REPOSITORIES: GEO

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Expression analysis of big neurospheres generated in vitro

Project description:Expression analysis of neurospheres generated in vitro treated by potassium chloride (KCl) and norepinephrine Neurospheres generated in vitro were treated with norepinephrine or potassium chloride. Gene expression analysis was then carried out to identify genes that are up or down regulated due to chemical treatment.

2015-06-12 | E-GEOD-67304 | biostudies-arrayexpress

Expression analysis of neurospheres generated in vitro

Project description:Neurospheres generated in vitro were treated with non-epinephrine or potassium chloride. Gene expression analysis was then carried out to identify genes that are up or down regulated due to chemical treatement. The microarray data for the neurosphere samples was generated at Queensland Brain Institute. Briefly, RNA was isolated from neurospheres generated in presence of either N (norepinephrine) or K (potassium chloride)and converted to cDNA using Applause WT-Amp ST kit (NuGEN). Fragmentation and biotin labelling of the cDNA was carried out using Encore Biotin module (NuGEN) for Affymetrix GeneChip Mouse Gene 1.0 ST arrays. Labelled cDNA was hybridised to arrays in an oven at 45M-BM-0C. Hybridised arrays were scanned using the Affymetrix GeneChip Scanner and the scanned data (.CEL files) was transferred to the Partek Genomics Suite and data analysis was carried out as described in Narayanan RK, Mangelsdorf M, Panwar A, Butler TJ, Noakes PG, et al. (2013).

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Expression analysis of neurospheres generated in vitro

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Drosophila melanogaster

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