Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

DNA methylation profiling of human THP-1 monocytes stimulated with arachidonic acid (AA) or oleic acid (OA) and controls

ABSTRACT: High-density array-based DNA methylation profiling of human THP-1 monocytes stimulated for 24 h with 1, 10 or 100uM AA, or the same concentrations of OA, and unstimulated controls. The Infinium HumanMethylation450 BeadChip was used to obtain DNA methylation profiles across approximately 485,000 CpGs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE67331 | GEO | 2015/03/27

SECONDARY ACCESSION(S): PRJNA279525

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

DNA methylation profiling of human THP-1 monocytes stimulated with arachidonic acid (AA) or oleic acid (OA) and controls

Project description:High-density array-based DNA methylation profiling of human THP-1 monocytes stimulated for 24 h with 1, 10 or 100uM AA, or the same concentrations of OA, and unstimulated controls. The Infinium HumanMethylation450 BeadChip was used to obtain DNA methylation profiles across approximately 485,000 CpGs. Bisulphite converted DNA was hybridised to the Illumina Infinium 450k Human Methylation Beadchip

2015-03-27 | E-GEOD-67331 | biostudies-arrayexpress

Expression data from human THP-1 monocytes stimulated with increasing concentrations of arachidonic (AA) or oleic acid (OA).

2014-04-27 | GSE57076 | GEO

Expression data from human THP-1 monocytes stimulated with increasing concentrations of arachidonic (AA) or oleic acid (OA).

Project description:Previous data indicated tha AA and OA modify global DNA methylation. This study analyzes the impact of these fatty acids on expression as a complement to array-based DNA methylation data. In this dataset, we include the expression data obtained from THP-1 monocyte stimulated with 1, 10 or 100uM AA or OA for 24h. RNA extracted from the same cells used for array-based DNA methylation analysis (see corresponding data in published article) was used to produce expression data by Affymetrix Human Exon 1.0 ST v2 Array

2014-04-27 | E-GEOD-57076 | biostudies-arrayexpress

Arachidonic and oleic acid exert distinct effects on the DNA methylome.

Project description: Not available

| S-EPMC4889238 | biostudies-literature

Nitro-Oleic Acid (NO2-OA) Improves Systolic Function in Dilated Cardiomyopathy by Attenuating Myocardial Fibrosis.

Project description: Not available

| S-EPMC8396484 | biostudies-literature

Nitro-Oleic Acid (NO2-OA) Release Enhances Regional Angiogenesis in a Rat Abdominal Wall Defect Model.

Project description: Not available

| S-EPMC5984564 | biostudies-literature

Study of HSA interactions with arachidonic acid using spectroscopic methods revealing molecular dynamics of HSA-AA interactions.

Project description: Not available

| S-EPMC7006104 | biostudies-literature

Brassica napus high oleic acid vs. low oleic acid

Project description:Compared to ordinary rapeseed, high-oleic acid rapeseed has higher levels of monounsaturated fatty acids and lower levels of saturated fatty acid and polyunsaturated fatty acids, and thus is of high nutritional and health value. In addition, high-oleic acid rapeseed oil imparts cardiovascular protective effects. Based on these properties, high-oleic acid oil crops have been extensively investigated and cultivated. In this study, we employed a microarray analysis with high oleic acid line and low oleic acid line from the developing seeds (27 days after flowering) of Brassica napus.

2018-09-20 | GSE120223 | GEO

Omega-6 fatty acids, arachidonic acid (AA) activates PI3K signaling and induces gene expression in prostate cancer

Project description:Essential fatty acids (FA) are not only energy-rich molecules; they are also an important component of the membrane bilayer and recently have been implicated in induction of fatty acid synthase (FAS) and other genes. Using gene chip analysis, we have found that arachidonic acid (AA), an omega-6 fatty acid, induced 11 genes that are regulated by NFkappaB. We verified gene induction by omega-6 fatty acids including COX2, IKBA, NFKB, GMCSF, IL1B, CXCL1, TNFA, IL6, LTA, IL8, PPARG, and ICAM1 using qRTPCR. PGE2 synthesis was increased within 5min of addition of AA. Analysis of upstream signal transduction showed that within 5min of FA addition, phophatidylinositol 3-kinase (PI3K) was significantly activated followed by activation of Akt at 30min. ERK1 and 2, p38, and SAPK/JNK were not phosphorylated after omega-6 FA addition. Thirty minutes after FA addition, we found a significant 3-fold increase in translocation of NFkappaB transcription factor to the nucleus. Addition of non-steroidal anti-inflammatory drug (NSAID) caused a decrease in cox-2 protein synthesis, PGE2 synthesis as well as inhibition of PI3K activation. We have previously shown that AA induced proliferation is also blocked (P<0.001) by PI3K inhibitor LY294002. LY294002 also significantly inhibited the AA induced gene expression of COX2, IL1B, GMCSF, and ICAM1. Taken together, the data suggests that AA via conversion to PGE2 plays an important role in stimulation of growth related genes and proliferation via PI3K signaling and NFkappaB translocation to the nucleus. Keywords: Time course of Prostate Cancer Activation by Arachidonic Acid

2006-01-01 | GSE3737 | GEO

HEWL interacts with dissipated oleic acid micelles, and decreases oleic acid cytotoxicity.

Project description: Not available

| S-EPMC6386356 | biostudies-literature