Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Impact of Lef1 overexpression on follicular helper T (Tfh) differentiation

ABSTRACT: Comparison of transcriptome between GFP-RV+ Th1 cells and Lef1-RV+ Th1 cells

ORGANISM(S): Mus musculus

PROVIDER: GSE67335 | GEO | 2015/07/27

SECONDARY ACCESSION(S): PRJNA279530

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Impact of Lef1 overexpression on follicular helper T (Tfh) differentiation

Project description:Comparison of transcriptome between GFP-RV+ Th1 cells and Lef1-RV+ Th1 cells B6 mice received GFP-RV+ or Lef1-RV+ LCMV gp specific TCRtg Smarta cells and were infected with LCMV. On four days after infection, splenocytes were collected to sort CXCR5- Th1 cells per genotype. Contributor: LIAI RNAi Center (LIAI)

2015-07-27 | E-GEOD-67335 | biostudies-arrayexpress

Impact of Tcf1 and Lef1 deficiency on follicular helper T (Tfh) cells

Project description:Comparison of transcriptome between control and Tcf1/Lef1-deficient germinal center Tfh (GC-Tfh) cells

2015-07-14 | GSE66781 | GEO

Tcf1 and Lef1 regulate follicular helper T cell function eliciated by vaccination [CUTnRUN]

Project description:The goal of this study is to determine the impact of Tcf1 and Lef1 deficiency on T follicular helper (Tfh) cells elicited by protein immunization. We demonstrate that although phenotypic Tcf1/Lef1-deficient Tfh cells were able to develop in response to vaccination, they are functionally impaired. Mechanisc studies reveal the direct regulatory effect by Tcf1 and Lef1 on transcriptome and chromatin accessibility in vaccine-elicited Tfh cells.

2020-10-19 | GSE146422 | GEO

Tcf1 and Lef1 regulate follicular helper T cell function eliciated by vaccination [ATAC-Seq]

2020-10-19 | GSE146421 | GEO

Tcf1 and Lef1 regulate follicular helper T cell function eliciated by vaccination [RNA-Seq]

2020-10-19 | GSE146425 | GEO

RNA-Seq of LEF1 overexpression ECA109 cells

Project description:RNA-seq was performed on three biological replicates. Total RNA from ECA109 LV-GFP/LV-LEF1.Differentially expression genes were considered to be significant between groups when the P-value was <0.05 and the fold change of expression was ≥2-fold or ≤0.5-fold. By this way, the RNA-Seq data showed the differential expression genes in LEF1 overexpression ECA109 cells compared with NC cells. Furthermore, Gene ontology (GO) analysis was performed to facilitate elucidating the biological implications of the differentially expressed genes in the experiment. Pathway analysis was used to find out the significant pathway of the differentially expressed genes according to KEGG database. In conclusion, our study represents the first detailed analysis of LEF1 transcriptomes in esophageal squamous cell carcinoma cells.

2020-04-10 | GSE128914 | GEO

TCF1 is required for the differentiation of T follicular helper (TFH) cells during viral infections

Project description:TFH and Th1 cells generated after viral or intracellular bacterial infections are critical for the control of infections and the development of immunological memories. However, the mechanisms that govern the choice of activated CD4 T cells to the two alternative fates remain unclear. Here, we found that reciprocal expression of TCF1 and Blimp1 between viral-specific TFH and Th1 cells started early after infection. TCF1 was intrinsically required for the differentiation of TFH cells. In the absence of TCF1, TFH cells failed to maintain their transcriptional and metabolic signatures, distinct from those in Th1 cells. Mechanistically, TCF1 functioned through forming negative feedback loops with IL-2 and Blimp1 signaling. Thus, we have demonstrated an essential role of TCF1 in TFH-cell differentiation. Tcf7 deficient and WT SMARTA CD4 T cells were isolated from mice 8 days after Lymphocytic Choriomeningitis Virus (LCMV) infection. TFH and Th1 cells were separated by FACS.

2015-09-18 | E-GEOD-65660 | biostudies-arrayexpress

Impact of Tcf1 and Lef1 deficiency on mature CD8 thymocytes

Project description:Comparison of transcriptome between control and Tcf1/Lef1-deficient mature CD8 thymocytes Control mice or those are deficient for Tcf1 and Lef1 transcription factors (deleted by CD4-Cre) were used to isolate thymocytes. The thymocytes were surface-stained to identify TCRbeta high, CD69â, CD24â CD8+ subsets. These cells were sorted for RNAseq analysis.

2016-02-12 | E-GEOD-73238 | biostudies-arrayexpress

Impact of Tcf1/Lef1 deficiency on Treg cells

Project description:Comparison of transcriptome between control and Tcf1/Lef1-deficient Treg cells

2019-02-14 | GSE119769 | GEO

Impact of Tcf1 and Lef1 deficiency on HSCs

Project description:Comparison of transcriptome between control and Tcf1/Lef1-deficient hematopoietic stem cells (HSCs).

2017-08-08 | GSE60587 | GEO