Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide analysis of chronic inflammation-induced gene expression in primary hepatocytes

ABSTRACT: Analysis of whole genome expression changes in primary hepatocytes in response to chronic stimulation with inflammatory cytokines. We hypothesized that chronic treatment of primary hepatocytes with TNFα would result in a reprogramming of the cell's transcriptome to improve adaptation to the presence of a chronic inflammatory stress. Here we provide expression analysis detailing genes upregulated, downregulated, and unchanged after 2 days of TNFα treatment. We have included gene expression profiling of cells treated with TNFα for 2 hours to help isolate the changes unique to chronic TNFα treatment of primary hepatocytes.

ORGANISM(S): Mus musculus

PROVIDER: GSE67422 | GEO | 2016/03/17

SECONDARY ACCESSION(S): PRJNA279860

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Genome-wide analysis of chronic inflammation-induced gene expression in primary hepatocytes

Project description:Analysis of whole genome expression changes in primary hepatocytes in response to chronic stimulation with inflammatory cytokines. We hypothesized that chronic treatment of primary hepatocytes with TNFÎ± would result in a reprogramming of the cell's transcriptome to improve adaptation to the presence of a chronic inflammatory stress. Here we provide expression analysis detailing genes upregulated, downregulated, and unchanged after 2 days of TNFÎ± treatment. We have included gene expression profiling of cells treated with TNFÎ± for 2 hours to help isolate the changes unique to chronic TNFÎ± treatment of primary hepatocytes. Total RNA isolated from primary hepatocytes cultured with 2ng/mL TNFÎ± for 2 hours or 2 days, compared to untreated cells

2016-03-17 | E-GEOD-67422 | biostudies-arrayexpress

IFN-b treatment of primary murine hepatocytes

Project description:IFN-b treatment of primary murine hepatocytes

| PRJNA628883 | ENA

Pro-inflammatory cytokine treatments in primary mouse hepatocytes

Project description:Pro-inflammatory cytokine treatments in primary mouse hepatocytes

| PRJNA379601 | ENA

miR1908-5p primary hepatocytes

Project description:miR1908-5p primary hepatocytes

| PRJNA745934 | ENA

Beauvericin (BEA) and enniatin B (ENNB)-induced impairment of mitochondria and lysosomes - potential sources of intracellular reactive iron triggering ferroptosis in Atlantic salmon primary hepatocytes

Project description:Beauvericin (BEA) and enniatin B (ENNB) are emerging mycotoxins frequently detected in plant-based fish feed. With ionophoric properties, they have shown cytotoxic potential in mammalian models. Sensitivity in fish is still largely unknown. Primary hepatocytes isolated from Atlantic salmon (Salmo salar) were used as a model and exposed to BEA and ENNB (0.05 – 10 µM) for 48 hours. Microscopy, evaluation of cell viability, total ATP, total H2O2, total iron content, total Gpx enzyme activity, and RNA sequencing were used to characterize the toxicodynamics of BEA and ENNB. Both mycotoxins became cytotoxic at ≥ 5 µM, causing condensation of the hepatocytes followed by formation of blister-like protrusions on the cell's membrane. RNA sequencing analysis at sub-cytotoxic levels indicated BEA and ENNB exposed hepatocytes to experience increased energy expenditure, elevated oxidative stress, and iron homeostasis disturbances sensitizing the hepatocytes to ferroptosis. The present study provides valuable knowledge disclosing the toxic action of these mycotoxins in Atlantic salmon primary hepatocytes.

2022-01-17 | GSE193374 | GEO

RNA sequencing in hepatocytes and DR-derived hepatocytes

Project description:During chronic liver injury, the ductular reaction generates hepatocytes. The aim of the analysis is to compare the phenotype of the DR-derived hepatocytes with that of native hepaotcytes in the same injured liver and control healthy hepatocytes.

2019-05-14 | GSE114436 | GEO

GH effects on primary hepatocytes from male rats

Project description:Effects of bGH treatment of primary hepatocytes cultured on matrigel Keywords: repeat sample

2005-05-04 | GSE2494 | GEO

Effect of Phenobarbital on Rat Primary Hepatocytes.

Project description:This study provides an evaluation of changes in gene expression associated with phenobarbital treatment of rat hepatocytes in vitro. Primary rat hepatocytes were treated for 24 and 48 hours with two doses (300 uM and 3 mM) of phenobarbital and water vehicle control. Five replicates of each treatment were performed. Cells were then extracted and RNA processed for microarray analysis. This series is part of a SuperSeries in which primary rat hepatocytes were treated with two doses of ten chemical compounds (and corresponding vehicle controls) for 24 and 48 hours. Each compound/vehicle treatment group was an individual study performed at different times. Each study was analyzed separately and themes common between studies were reported. Time Course/Dose Response

2012-08-28 | E-GEOD-40339 | biostudies-arrayexpress

Comparative gene expression profiles induced by PPARγ and PPARα/γ agonists in human hepatocytes

Project description:Transcriptomic profiling of primary human hepatocytes after treatment with 2 glitazones (TRO and ROSI) and 2 glitazars (MURA and TESA) Singe-Channel Samples: We have analysed changes in gene expression profiles in primary human hepatocytes after a 24 h treatment with various concentrations of glitazones and glitazars Dual-Channel Samples: We have analysed changes in gene expression profiles in primary human hepatocytes and differentiated human hepatoma HepaRG cells after a 24 h treatment with various concentrations of glitazones and glitazars

2014-02-09 | GSE27183 | GEO

GH effects on primary hepatocytes from male rats

Project description:Effects of bGH treatment of primary hepatocytes cultured on matrigel

2010-06-10 | E-GEOD-2494 | biostudies-arrayexpress