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Transcription Profiling of Malaria-Naïve and Semi-Immune Colombian Volunteers in a Plasmodium vivax Sporozoite Challenge [RT-PCR]

ABSTRACT: Whole blood gene expression was monitored in 85 samples using a Fluidigm nanoscale RT-qPCR array targeting 96 genes that we refer to as “blood informative transcripts” (BIT). There was no evidence for transcriptional changes prior to the appearance of blood stage parasite at day 12 or 13, at which time there was a strong interferon response and, unexpectedly, down-regulation of transcripts related to inflammation and innate immunity. This samples are related with the samples with accession number GSE67184

ORGANISM(S): Homo sapiens

PROVIDER: GSE67469 | GEO | 2015/07/14

SECONDARY ACCESSION(S): PRJNA280001

REPOSITORIES: GEO

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Transcription Profiling of Malaria-Naïve and Semi-Immune Colombian Volunteers in a Plasmodium vivax Sporozoite Challenge [RT-PCR]

Project description:Whole blood gene expression was monitored in 85 samples using a Fluidigm nanoscale RT-qPCR array targeting 96 genes that we refer to as “blood informative transcripts” (BIT). There was no evidence for transcriptional changes prior to the appearance of blood stage parasite at day 12 or 13, at which time there was a strong interferon response and, unexpectedly, down-regulation of transcripts related to inflammation and innate immunity. This samples are related with the samples with accession number GSE67184 Reverse Transcription followed by quantitative PCR (RT-qPCR) was performed using Fluidigm 96×96 nanofluidic arrays targeting a set of 96 transcripts that are broadly informative of the major axes of variation for peripheral blood gene expression from Preininger et al. (2013) at six time-points (Pre-challenge, day 5, day 7, day 9, Diagnosis (Dx) and month 4).

2015-07-14 | E-GEOD-67469 | biostudies-arrayexpress

Plasmodium vivax sporozoite challenge in malaria-naive and semi-immune Colombian volunteers.

Project description: Not available

| S-EPMC4070897 | biostudies-literature

Transcription Profiling of Malaria-Naïve and Semi-Immune Colombian Volunteers in a Plasmodium vivax Sporozoite Challenge [RNA-Seq]

Project description:We report two clusters in the overall profiles of expression among the samples. At the parasitemia onset, there is a strong interferon response reflected in up-regulation of co-regulated transcripts, while unexpectedly we also see down-regulation of transcripts related to TLR signaling and innate immunity. RNASeq also suggested differential expression of reticulocytes and a subset of T cell function. No obvious difference in the transcriptomes of naïve and semi-immune volunteers was seen, however several hundred genes were up-regulated in naïve individuals.

2015-07-14 | GSE67184 | GEO

Transcription Profiling of Malaria-Naïve and Semi-Immune Colombian Volunteers in a Plasmodium vivax Sporozoite Challenge

Project description:This SuperSeries is composed of the SubSeries listed below.

2015-07-14 | GSE67470 | GEO

Transcription Profiling of Malaria-Naïve and Semi-Immune Colombian Volunteers in a Plasmodium vivax Sporozoite Challenge [RNA-Seq]

2015-07-14 | E-GEOD-67184 | biostudies-arrayexpress

Transcription Profiling of Malaria-Naive and Semi-immune Colombian Volunteers in a Plasmodium vivax Sporozoite Challenge.

Project description: Not available

| S-EPMC4526565 | biostudies-literature

Transcription Profiling of Malaria-Naïve and Semi-Immune Colombian Volunteers in a Plasmodium vivax Sporozoite Challenge

Project description:Transcription Profiling of Malaria-Naïve and Semi-Immune Colombian Volunteers in a Plasmodium vivax Sporozoite Challenge

| PRJNA280000 | ENA

Profiling gene expression of the host response to an irradiated sporozoite immunization and Plasmodium vivax malaria challenge

Project description:We report an initial understanding of the modular immune response to a P. vivax malaria challenge after protection with PvRAS. By contrasting blood samples drawn at baseline, following immunization but pre-challenge, and at the first day of diagnosis of malaria in affected subjects.

2017-12-31 | GSE85263 | GEO

Protective Efficacy of Plasmodium vivax Radiation-Attenuated Sporozoites in Colombian Volunteers: A Randomized Controlled Trial.

Project description: Not available

| S-EPMC5070852 | biostudies-literature

Unraveling the Plasmodium vivax sporozoite transcriptional journey from mosquito vector to human host

Project description:Malaria parasites transmitted by mosquito bite are remarkably efficient in establishing human infections. The infection process requires ~30 minutes and is highly complex as quiescent sporozoites injected with mosquito saliva must be rapidly activated in the skin, migrate through the body, and infect the liver. This process is poorly understood for Plasmodium vivax due to low infectivity in the in vitro models. To study this skin-to-liver stage of malaria, we developed quantitative bioassays coupled with transcriptomics to evaluate parasite changes linked with mammalian microenvironmental factors. Our in vitro phenotype and RNA-seq analyses revealedkey microenvironmental relationships with distinct biological functions. M ost notable, preservation of sporozoite quiescence by exposure to insect-like factors coupled with strategic activation limits untimely activation of invasion-associated genes to dramatically increase hepatocyte invasion rates. We also report the first transcriptomic analysis of the P. vivax sporozoite interaction in salivary glands identifying 118 infection-related differentially-regulated Anopheles dirus genes. These results provide important new insights in malaria parasite biology and identify priority targets for antimalarial therapeutic interventions to block P. vivax infection.

2018-08-16 | GSE117538 | GEO

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