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3' RNA-seq of flies over-expressing cabut protein or RNAi against cabut mRNA

ABSTRACT: CbtOE (Tim-gal4; UAS-cbtFLAG), Tim-gal4 (control for CbtOE), cbtRNAi (Tim-gal4-UAS-Dcr2-UAS-cbtIR-cbtE1) and Tim-gal4;UAS-Dcr2 (control for CbtRNAi) flies. Flies were entrained in LD (light: dark) condition for 3-4 days and harvested at six time points: ZT3, ZT7, ZT11, ZT15, ZT19, ZT23 Fly heads were collected, RNA was extracted and RNA-seq libraries were prepared as previously described (Engreitz et al., 2013)

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE67590 | GEO | 2015/05/08

SECONDARY ACCESSION(S): PRJNA280389

REPOSITORIES: GEO

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3' RNA-seq of flies over-expressing cabut protein or RNAi against cabut mRNA

Project description:CbtOE (Tim-gal4; UAS-cbtFLAG), Tim-gal4 (control for CbtOE), cbtRNAi (Tim-gal4-UAS-Dcr2-UAS-cbtIR-cbtE1) and Tim-gal4;UAS-Dcr2 (control for CbtRNAi) flies. Flies were entrained in LD (light: dark) condition for 3-4 days and harvested at six time points: ZT3, ZT7, ZT11, ZT15, ZT19, ZT23 Fly heads were collected, RNA was extracted and RNA-seq libraries were prepared as previously described (Engreitz et al., 2013) Three samples of cbtRNAi and three samples of their controls. Two samples of cbtOE with two samples of their controls.

2015-05-08 | E-GEOD-67590 | biostudies-arrayexpress

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Project description:WT flies or flies of the strain Tim-gal4; UAS-MJD78Q. All samples were collected at ZT16 after 3 days of training in LD conditions.

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WT Vs TIM-MJD

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mRNA Expression Data from Murine Hearts of Clock delta 19/delta 19 Mice over 24 hours in normal light-dark environment (12:12)

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mRNA Expression Data from Murine Hearts of Wildtype Mice over 24 hours in normal light-dark environment (12:12)

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3' RNA-seq of fly expressing cabut RNAi after exposure to different levels of sucrose

Project description:Control (+/cbtE1-UAS-cbt RNAi) or cabut RNAi flies (Tim-gal4, UAS-cbt RNAi) were starved for 16 hours and then exposed to food containing different concentrations of sucrose: 0, 25, 50 and 100 % for 18 hours. Fly heads were collected, RNA was extracted and RNA-seq libraries were prepared as previously described (Engreitz et al., 2013) For each sucrose concentration, two samples of cabut RNAi flies and one sample of control flies were sequenced.

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Global quantitative proteomic analysis of aging Drosophila eyes

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3' RNA-seq of fly expressing cabut RNAi after exposure to different levels of sucrose

2015-05-08 | GSE58854 | GEO

CLK targets from fly heads

Project description:CLK targets from fly heads using the TIM-GAL4; UAS-CLKGR line Keywords: CLK TARGETS

2007-07-09 | GSE7646 | GEO

Transcription profiling of Drosophila fly heads treated with CHX, dexamethasone vs. controls

Project description:CLK targets from fly heads using the TIM-GAL4; UAS-CLKGR line Experiment Overall Design: Fly heads were stimulated with vehicle + CHX or dexamethasone + CHX

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