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Untreated and iron-treated ARPE-19 cell gene expression

ABSTRACT: To characterize the potential molecular pathway(s) affected by iron treatment and identify the one(s) responsible for C3 induction, we performed a whole genome microarray on untreated ARPE-19 cells and cells treated with 250 μM FAC for 48h/2d.

ORGANISM(S): Homo sapiens

PROVIDER: GSE67603 | GEO | 2015/04/07

SECONDARY ACCESSION(S): PRJNA280416

REPOSITORIES: GEO

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Untreated and iron-treated ARPE-19 cell gene expression

Project description:To characterize the potential molecular pathway(s) affected by iron treatment and identify the one(s) responsible for C3 induction, we performed a whole genome microarray on untreated ARPE-19 cells and cells treated with 250 ?M FAC for 48h/2d. Gene expression was compared between untreated and FAC-treated ARPE-19 cells, with three biological replicates in each.

2015-04-07 | E-GEOD-67603 | biostudies-arrayexpress

ARPE-19_adriamycin_gene_expression

Project description:This study compared the gene expression change of ARPE-19 cells before and after adriamycin treatment ARPE-19 cells were treated with 1Î¼M adriamycin for 24 h compared with control

2016-03-01 | E-GEOD-78765 | biostudies-arrayexpress

ARPE-19_adriamycin_gene_expression

Project description:This study compared the gene expression change of ARPE-19 cells before and after adriamycin treatment

2016-03-01 | GSE78765 | GEO

RNA-seq to investigate the effect of curcumin on hypoxia-induced proliferation of human retinal pigment epithelium ARPE-19 cells

Project description:ARPE-19 RPE cells obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China) were cultured in DMEM medium with 10% FBS (Thermo Fisher Scientific, Waltham, USA) at the incubator with 37 °C, 5% CO2 and 100% humidity. Final concentration of 15 μM curcumin (C7727, Sigma-Aldrich, St. Louis, USA) and 100 μM CoCl4 (10007216, Sinopharm Chemical Reagent, Shanghai, China) were added within serum-free medium for 24 h and 4 h respectively. RNA-seq was performed to investigate the transcriptome alteration.

2020-10-24 | E-MTAB-8481 | biostudies-arrayexpress

ARPE-19 adriamycin gene expression II

Project description:This study compared the gene expression change of ARPE-19 cells before and after adriamycin treatment

2017-10-06 | GSE104662 | GEO

Iron regulation in Vibrio vulnificus CMCP6

2008-08-07 | GSE10633 | GEO

Iron regulation in Vibrio vulnificus CMCP6

Project description:Vibrio vulnificus multiply rapidly in host tissues under iron overloaded conditions. To understand the effects of iron in the physiology of this pathogen we performed a genome-wide transcriptional analysis of this bacterium growing under three different iron concentrations. V.vulnificus CMCP6 cells were grown under three different iron concentrations (TSBS + EDDA 50uM, TSBS and TSBS + FAC 250 ug/ml) and samples taken at log phase. Keywords: Response to the iron concentration of the media Strains were grown to an OD600nm of 0.6 to 0.8 in TSBS, TSBS with the addition of 250 Î¼g/ml FAC or TSBS with the addition of 50 Î¼M EDDA. Three independent cultures of the V. vulnificus cells grown in each media, were combined and treated as a single sample for the RNA extraction to minimize culture variation. Two samples per condition were used for the microarray analysis. Cells were centrifuged and the pellets resuspended in RNAWiz reagent (AmbionÂ®, Austin, TX). Total RNA was extracted from each strain according to the manufacturerâ??s instructions

2010-05-25 | E-GEOD-10633 | biostudies-arrayexpress

Gene expression signitures in ARPE-19 cells treated with or without TGF-β1

Project description:To identify the dysregulated lncRNA and mRNA expression in ARPE-19 cells underwent EMT, we established a TGF-β1 induced EMT model of ARPE-19 cells. ARPE-19 cells were treated with or without 10 ng/ml TGF-β1 for 48 h. Total RNA are extracted and subjected to microarray assay (Arraystar Human LncRNA Microarray V3.0)

2021-08-11 | GSE105053 | GEO

Expression Data from DMSO or SRPIN803 treated ARPE-19 cells

Project description:We used microarrays to evaluate the effect of SRPIN803 on gene expression in ARPE-19 cells. ARPE-19 cells were treated with SRPIN803 (10 uM) or the negative control (0.1% DMSO) for 4 hours for Total RNA isolation and hybridization on Microarray.

2015-06-17 | E-GEOD-62947 | biostudies-arrayexpress

ChIA-PET from ARPE-19 (ENCSR110JOO)

Project description:RAD21 ChIA-PET in human ARPE-19 cells For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2019-03-03 | GSE127017 | GEO

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